An Improved Diluent for Rubella Hemagglutination and Hemagglutination-Inhibition Tests [PDF]
Rubella hemagglutinating (HA) antigen was prepared in BHK-21 tissue as 5% cell suspensions and from unconcentrated and 20× concentrated infected supernatant fluids. In some instances, unconcentrated fluids were treated with Tween 80 and ether; cell suspensions were treated with ether alone.
Angela E. Auletta +3 more
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Standardized Viral Hemagglutination and Hemagglutination-Inhibition Tests. I. Standardization of Erythrocyte Suspensions [PDF]
A spectrophotometric procedure for standardizing red blood cell suspensions for use in viral hemagglutination tests is described. The procedure is based on highly reproducible cyanmethemoglobin absorbance readings at 540 nm on any suitable spectrophotometer.
John C. Hierholzer, Morris T. Suggs
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Analysis of the Diluents Used for Rubella Virus Hemagglutination and Hemagglutination-Inhibition Tests [PDF]
[This corrects the article on p. 222 in vol. 18.].
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DIAGNOSIS OF SMALLPOX BY THE HEMAGGLUTINATION INHIBITION TEST
Vaccinia virus preparations agglutinate chicken erythrocytes and this hemagglutination is specifically inhibited by antivaccinial sera. Since the inhibition of helnagglutinanoit by antisera may be one of the most sensitive reactions as a method for detecting the specific antibody, it seems probable that this reaction may be applied to the diagnosis for
Morihide Yamada +4 more
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Comparative Analysis of Hemagglutination Inhibition and Plaque Reduction Neutralization Tests for Japanese Encephalitis Virus Antibody Detection [PDF]
Japanese encephalitis (JE) is a zoonotic disease caused by the Japanese encephalitis virus (JEV), belonging to the Flaviviridae family. Diagnosis of Japanese encephalitis (JE) based on clinical signs alone is challenging due to the high proportion of ...
Cui Li +6 more
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Cryopreserved erythrocytes in clinical laboratory hemagglutination and hemagglutination inhibition tests [PDF]
Various concentrations of pig, guinea pig, chicken, and turkey erythrocytes (RBCs) were cryopreserved and compared with fresh RBCs in hemagglutination and hemagglutination inhibition tests. Hemagglutination and hemagglutination inhibition titers obtained with cryopreserved or fresh RBCs from the same donor differed by not more than one twofold dilution.
Roger K. Maes +2 more
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Co-trimoxazole-dependent autoagglutinin with anti-Sda specificity detected only by column agglutination technique [PDF]
BACKGROUND AND OBJECTIVES: In vitro serological reactions due to antibodies in the presence of certain chemicals are rare occurrences. Although such antibodies are often considered harmless, their innocuous nature requires verification. We investigated a
Revathy K. Nair +4 more
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Simple hemagglutination inhibition test for the diagnosis of toxoplasmosis [PDF]
A simple hemagglutination inhibition (HI) test for the serological diagnosis of toxoplasmosis has been developed and evaluated. A total of 84 human and 120 mouse serum samples were tested by the newly developed HI test and compared with an immunoglobulin G-indirect fluorescent antibody test. Statistical analysis of serum titers obtained by using the HI
Gan-Nan Chang +3 more
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Standardized Viral Hemagglutination and Hemagglutination-Inhibition Tests. II. Description and Statistical Evaluation [PDF]
Standardized hemagglutination and hemagglutination-inhibition procedures are described and statistically evaluated for all animal viruses where applicable, except for rubella and the arbovirus group. The standardized tests employ a constant phosphate-buffered saline diluent and constant volumes of serum, antigen, and standardized erythrocyte suspension.
John C. Hierholzer +2 more
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Seroprevalence of mycoplasmosis in broiler, layer, and native chickens in Giza, Egypt.
We aimed to investigate Mycoplasma infections among chicken flocks (Ross, Lohmann and native) in Giza, Egypt, using serological tests, including the slide plate agglutination (SPA) test, hemagglutination inhibition (HI) test, and enzyme-linked ...
Saeed El-Ashram +4 more
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