Results 81 to 90 of about 2,618 (230)

Observation of the FeO2 and FeIVO stretching Raman bands for dioxygen reduction intermediates of cytochrome bo isolated from Escherichia coli [PDF]

, 1994
Reaction intermediates in dioxygen reduction by the E. coli cytochrome bo-type ubiquinol oxidase were studied by time-resolved resonance Raman spectroscopy using the artificial cardiovascular system.
Anraku, Yasuhiro, Hirano, Tomoyasu, Hirota, Shun, Kitagawa, Teizo, Mogi, Tatsushi, Ogura, Takashi   +5 more
core   +1 more source

Fungal systems for lignocellulose deconstruction: From enzymatic mechanisms to hydrolysis optimization

GCB Bioenergy, Volume 16, Issue 5, May 2024.
Biomass contains complex sugars and aromatic polymers that have the potential to serve as sustainable alternatives to fossil fuels and chemicals. Fungi possess specialized enzyme systems capable of breaking down lignocellulose into fermentable building blocks. This review analyses recent advancements in intricate fungal decomposition toolkits, shedding
Fengyun Ren, Fan Wu, Xin Wu, Tongtong Bao, Yucheng Jie, Le Gao   +5 more
wiley   +1 more source

Exploring protein phosphorylation by combining computational approaches and biochemical methods

Computational and Structural Biotechnology Journal, 2020
Post-translational modifications of proteins expand their functional diversity, regulating the response of cells to a variety of stimuli. Among these modifications, phosphorylation is the most ubiquitous and plays a prominent role in cell signaling.
Gonzalo Pérez-Mejías, Alejandro Velázquez-Cruz, Alejandra Guerra-Castellano, Blanca Baños-Jaime, Antonio Díaz-Quintana, Katiuska González-Arzola, Miguel Ángel De la Rosa, Irene Díaz-Moreno   +7 more
doaj  

Conformational Dependence of a Protein Kinase Phosphate Transfer Reaction

, 2004
Atomic motions and energetics for a phosphate transfer reaction catalyzed by the cAMP-dependent protein kinase (PKA) are calculated by plane-wave density functional theory, starting from structures of proteins crystallized in both the reactant ...
Austin, B. H. McMahon, C.-S. Tung, Cheng, Clementi, Frauenfelder, Frauenfelder, G. Henkelman, Hanggi, Johnson, Johnson, M. X. LaBute, Madhusudan, McMahon, N  meth, P. W. Fenimore, Pearson, Pinna, Smith, Songyang, Valiev, Yang, Zheng   +22 more
core   +2 more sources

Influence of the freezing process upon fluoride binding to hemeproteins [PDF]

Biophysical Journal, 1991
Fluoride association with ferric myoglobins and hemoglobins in aqueous buffers above freezing has been well studied. We chose this reaction to investigate the feasibility of observing titration intermediates and estimating dissociation constants at the freezing temperature by electron paramagnetic resonance spectroscopy at cryogenic temperatures ...
An-Suei Yang, Arthur S. Brill
openaire   +2 more sources

Cultivating the uncultured: Harnessing the “sandwich agar plate” approach to isolate heme‐dependent bacteria from marine sediment

mLife, Volume 3, Issue 1, Page 143-155, March 2024.
Abstract In the classical microbial isolation technique, the isolation process inevitably destroys all microbial interactions and thus makes it difficult to culture the many microorganisms that rely on these interactions for survival. In this study, we designed a simple coculture technique named the “sandwich agar plate method,” which maintains ...
Jing Zhang, Qi‐Yun Liang, Da‐Shuai Mu, Fengbai Lian, Ya Gong, Mengqi Ye, Guan‐Jun Chen, Yuqi Ye, Zong‐Jun Du   +8 more
wiley   +1 more source

Reactive oxygen-related diseases: therapeutic targets and emerging clinical indications [PDF]

, 2015
SIGNIFICANCE Enhanced levels of reactive oxygen species (ROS) have been associated with different disease states. Most attempts to validate and exploit these associations by chronic antioxidant therapies have provided disappointing results. Hence, the
Ana I. Casas, Andreas Daiber, Antonio Cuadrado, Brunner HG, Fabio Di Lisa, Ghassan J. Maghzal, Hampton MB, Harald H.H.W. Schmidt, Hou YC, Karl H. Krause, Klebanoff SJ., Liu VW, Manuela G. López, Nina Kaludercic, Pietro Ghezzi, Roland Stocker, Sandler M, Savenkova ML, Sonia Leach, Tamara Seredenina, V. Thao-Vi Dao, Vincent Jaquet   +21 more
core   +2 more sources

Mechanistics of pH‐Dependent Sulfmyoglobin Formation: Spin Control and His64 Proton Relay

Journal of Chemistry, Volume 2024, Issue 1, 2024.
The chemistry of hydrogen sulfide (H2S) has been directed towards physiologically relevant hemeproteins, including myoglobin, hemoglobin, and other similar proteins. Despite substantial efforts, there remains a need to elucidate the mechanism and identify the species involved in the reaction between oxy‐hemeproteins and H2S. Here, we summarize both our
Angel D. Rodriguez-Mackenzie, Lysmarie Santos-Velazquez, Héctor D. Arbelo-Lopez, Troy Wymore, Juan Lopez-Garriga, Jose Corchado   +5 more
wiley   +1 more source

Heme Spin Distribution in the Substrate-Free and Inhibited Novel CYP116B5hd: A Multifrequency Hyperfine Sublevel Correlation (HYSCORE) Study

Molecules
The cytochrome P450 family consists of ubiquitous monooxygenases with the potential to perform a wide variety of catalytic applications. Among the members of this family, CYP116B5hd shows a very prominent resistance to peracid damage, a property that ...
Antonino Famulari, Danilo Correddu, Giovanna Di Nardo, Gianfranco Gilardi, George Mitrikas, Mario Chiesa, Inés García-Rubio   +6 more
doaj   +1 more source

Genetically Tunable Enzymatic C‒H Amidation for Lactam Synthesis [PDF]

, 2019
A major challenge in carbon‒hydrogen (C‒H) bond functionalization is to have the catalyst control precisely where a reaction takes place. Here we report engineered cytochrome P450 enzymes that perform unprecedented enantioselective C‒H amidation ...
Arnold, Frances H., Cho, Inha, Jia, Zhi-Jun   +2 more
core