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On Using Active Contour to Segment HEp-2 Cells
2016 IEEE 29th International Symposium on Computer-Based Medical Systems (CBMS), 2016The development of computer-aided diagnosis (CAD) systems for antinuclear autoantibodies tests in indirect immunofluorescence using HEp-2 cells has attracted growing research efforts in the last years. Although in this field many CAD solutions extract information from objects detected within the images, cell segmentation is an issue far from being ...
Mario Merone, Paolo Soda
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1985
Abstract Hep-2 cells are commonly used as substrate in immunofluorescence tests. At the ultrastructural level, the nucleus of HEp-2 cell is characterized by a small amount of dense chromatin and, in the interchromatinic areas, by large nucleoli and by several types of nuclear bodies.
M. THIRY, R. HUMBEL, G. GOESSENS
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Abstract Hep-2 cells are commonly used as substrate in immunofluorescence tests. At the ultrastructural level, the nucleus of HEp-2 cell is characterized by a small amount of dense chromatin and, in the interchromatinic areas, by large nucleoli and by several types of nuclear bodies.
M. THIRY, R. HUMBEL, G. GOESSENS
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Effects of Helicobacter hepaticus on the proteome of HEp-2 cells
Antonie van Leeuwenhoek, 2007Helicobacter hepaticus infects the bowel and biliary tree of several animals, producing inflammation. Colonisation of mouse livers can induce hepatocellular carcinomas. The effects of H. hepaticus on the proliferation and global protein expression of human HEp-2 cells were studied by examining the changes in the protein profiles of cells exposed to the
Arinze S, Okoli +3 more
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Adherence and internalization of Helicobacter pylori by HEp-2 cells
Gastroenterology, 1992Helicobacter pylori colonizes the mucous layer of the stomach and the surface of gastric mucous cells. Although H. pylori is not generally thought of as invasive, it has been observed in the lamina propria and within vacuoles in the cytoplasm of epithelial cells. The authors report that isolates of H.
D G, Evans, D J, Evans, D Y, Graham
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Haemophilus influenzae triggers autophagy in HEp-2 cells
Archives of Microbiology, 2015The MAP-LC3 system regulates the intracellular formation of autophagy-associated vacuoles. These vacuoles contain the LC3 protein; thus it has been utilized as a marker to identify autophagosomes. The aim of our study was to investigate whether Haemophilus influenzae strains and their supernatants could activate autophagy in human larynx carcinoma cell
María del Rosario, Espinoza-Mellado +5 more
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Early experiences in mitotic cells recognition on HEp-2 slides
2010 IEEE 23rd International Symposium on Computer-Based Medical Systems (CBMS), 2010Indirect immunofluorescence (IIF) imaging is the recommended laboratory technique to detect autoantibodies in patient serum, but it suffers from several issues limiting its reliability and reproducibility. IIF slides are observed by specialists at the fluorescence microscope, reporting fluorescence intensity and staining pattern and looking for mi ...
FOGGIA, PASQUALE +3 more
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Segmentation of HEp-2 cells centromere pattern
2017 2nd IEEE International Conference on Recent Trends in Electronics, Information & Communication Technology (RTEICT), 2017According to NIH, an autoimmune diseases have infected almost 23.5 million peoples of the USA. The detection of such autoimmune disease is done by using Antinuclear Antibodies test of Human Epithelial pattern 2 (HEp-2) cells. It is needed to design an automatic system for ANA testing, which involves the stage of segmentation of HEp-2 cells.
Muley Jayant Arun, Varsha R Ratnaparkhe
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HEp-2 Cell Image Classification: A Comparative Analysis
2013HEp-2 cell image classification is an important and relatively unexplored area of research. This paper presents an experimental analysis of five different categories of feature sets with four different classifiers to determine the best performing combination of features and classifiers.
Praful Agrawal +2 more
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Classification of HEp-2 cells using distributed dictionary learning
2016 24th European Signal Processing Conference (EUSIPCO), 2016Automatic classification of human epithelial type-2 (HEp-2) cells can improve the diagnostic process of autoimmune diseases (ADs) in terms of lower cost, faster response, and better repeatability. However, most of the proposed methods for classification of HEp-2 cells suffer from several constraints including tedious parameter tuning, massive memory ...
Sadaf Monajemi +6 more
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Automatic CAD System for HEp-2 Cell Image Classification
2014 22nd International Conference on Pattern Recognition, 2014It has been estimated that autoimmune diseases are among the top ten leading causes of death among women in all age groups up to 65 years. However, the detection of it by indirect immunofluorescence (IIF) image analysis depends heavily on the experience of the physicians. An accurate and automatic Computer Aided Diagnosis (CAD) system will help greatly
Shahab Ensafi +3 more
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