Results 351 to 360 of about 541,993 (403)
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Microencapsulating Hepatocytes
Transplantation Proceedings, 2005Because standardization of the cell microencapsulation procedure has not yet been achieved, we performed hepatocyte microencapsulation using alginate (ALG)-poly-l-lysine (PLL)-ALG (APA) polymer.Hepatocytes were microencapsulated using a binozzle air-jet droplet generator.
Y, Gao +4 more
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2009
Apoptosis has been documented as a frequent hurdle phenomenon that occurs in human hepatocytes during isolation, storage, infusion and after engraftment within the recipient liver parenchyma. Apoptosis is an active form of cell death that involves programmed cellular machineries leading to a progressive self-destruction of the cell.
Mustapha, Najimi +2 more
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Apoptosis has been documented as a frequent hurdle phenomenon that occurs in human hepatocytes during isolation, storage, infusion and after engraftment within the recipient liver parenchyma. Apoptosis is an active form of cell death that involves programmed cellular machineries leading to a progressive self-destruction of the cell.
Mustapha, Najimi +2 more
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Journal of Gastroenterology and Hepatology, 2002
Summary Repopulation of the liver with transplanted cells holds significant promise for developing novel therapies. The liver is a most suitable target for treating a variety of genetic, metabolic and acquired diseases. Liver disease, such as chronic viral hepatitis, constitutes an enormous burden worldwide.
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Summary Repopulation of the liver with transplanted cells holds significant promise for developing novel therapies. The liver is a most suitable target for treating a variety of genetic, metabolic and acquired diseases. Liver disease, such as chronic viral hepatitis, constitutes an enormous burden worldwide.
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2006
Isolated hepatocytes are a physiologically relevant in vitro model exhibiting intact subcellular organelles, xenobiotic transport, and integrated phase I and phase II biotransformation. They represent the "gold standard" for investigating xenobiotic biotransformation and metabolic bioactivation.
Elaut, Greetje +6 more
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Isolated hepatocytes are a physiologically relevant in vitro model exhibiting intact subcellular organelles, xenobiotic transport, and integrated phase I and phase II biotransformation. They represent the "gold standard" for investigating xenobiotic biotransformation and metabolic bioactivation.
Elaut, Greetje +6 more
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Artificial Cells, Blood Substitutes, and Biotechnology, 1999
Homogenized rat hepatocytes, whole hepatocytes and encapsulated hepatocytes were incubated with bilirubin and UDP-glucuronic acid to test their ability to form bilirubin conjugates. Bilirubin monoconjugated and diconjugated were detected in all the three preparations by HPLC analysis.
S, Bruni, T M, Chang
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Homogenized rat hepatocytes, whole hepatocytes and encapsulated hepatocytes were incubated with bilirubin and UDP-glucuronic acid to test their ability to form bilirubin conjugates. Bilirubin monoconjugated and diconjugated were detected in all the three preparations by HPLC analysis.
S, Bruni, T M, Chang
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Current Protocols in Toxicology, 2001
AbstractThis unit describes a process for isolation of intact, viable hepatocytes for use in integrated drug metabolism studies. Isolated hepatocytes are increasingly being used as a biological system for studying the in vitro metabolism of xenobiotics.
D R, Mudra, A, Parkinson
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AbstractThis unit describes a process for isolation of intact, viable hepatocytes for use in integrated drug metabolism studies. Isolated hepatocytes are increasingly being used as a biological system for studying the in vitro metabolism of xenobiotics.
D R, Mudra, A, Parkinson
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Hepatology, 1992
The two papers in this issue of Hepatology (1, 2) dealing with hepatocyte growth factor (HGF) underscore the increasing importance of this novel growth factor in relation to hepatic growth biology. The emerging literature has already established HGF as a growth factor with potential importance not only for the liver but for ...
G K, Michalopoulos, R, Zarnegav
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The two papers in this issue of Hepatology (1, 2) dealing with hepatocyte growth factor (HGF) underscore the increasing importance of this novel growth factor in relation to hepatic growth biology. The emerging literature has already established HGF as a growth factor with potential importance not only for the liver but for ...
G K, Michalopoulos, R, Zarnegav
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2006
Primary culture of human hepatocytes is an in vitro model widely used to investigate numerous aspects of liver physiology and pathology. The technique used to isolate human hepatocytes is based on two-step collagenase perfusion. Originally performed in situ for obtaining hepatocytes from the adult rat, this technique has been adapted to the ex vivo ...
Lydiane, Pichard +5 more
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Primary culture of human hepatocytes is an in vitro model widely used to investigate numerous aspects of liver physiology and pathology. The technique used to isolate human hepatocytes is based on two-step collagenase perfusion. Originally performed in situ for obtaining hepatocytes from the adult rat, this technique has been adapted to the ex vivo ...
Lydiane, Pichard +5 more
openaire +2 more sources
Human Hepatocyte Transplantation
2010Over the last decade the interest in hepatocyte transplantation has been growing continuously and this treatment may represent an alternative clinical approach for patients with acute liver failure and life-threatening liver-based metabolic disorders. The technology also serves as the proof of concept and reference for future development in stem cell ...
Anil, Dhawan +3 more
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1996
The use of in vitro and in vivo systems to evaluate hepatic drug uptake and metabolism, cytochrome P450 induction, drug interactions affecting hepatic metabolism, hepatotoxicity, and cholestasis is an essential part of the drug development process (Tavoloni and Boyer, 1980; Powis et al., 1989; Bertrams and Ziegler, 1991; Jurima-Romet and Huang, 1992 ...
E L, LeCluyse +3 more
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The use of in vitro and in vivo systems to evaluate hepatic drug uptake and metabolism, cytochrome P450 induction, drug interactions affecting hepatic metabolism, hepatotoxicity, and cholestasis is an essential part of the drug development process (Tavoloni and Boyer, 1980; Powis et al., 1989; Bertrams and Ziegler, 1991; Jurima-Romet and Huang, 1992 ...
E L, LeCluyse +3 more
openaire +2 more sources