Results 171 to 180 of about 8,443 (253)

Coupling of both a transactivation module and a double‐stranded DNA‐binding domain boosts Cas12i3 variant‐based cytosine and adenine editing in plants

open access: yesJournal of Integrative Plant Biology, EarlyView.
Fusions of a transactivation module and a double‐stranded DNA‐binding domain produced effective Cas12i3 variant‐based cytosine and adenine base editors, with improved C‐to‐T and A‐to‐G base editing efficiency and expanded editing window. Generated herbicide‐resistant rice germplasm by using these base editors demonstrates their potential for precise ...
Chen Zhang   +7 more
wiley   +1 more source

Ubiquitous presence of pesticides in feathers of common UK birds. [PDF]

open access: yesEnviron Sci Pollut Res Int
Tank P   +4 more
europepmc   +1 more source

Synergistic engineering of Casδ nuclease for robust genome editing

open access: yesJournal of Integrative Plant Biology, EarlyView.
A hierarchical engineering strategy was used to enhance the editing activity of Casδ, yielding an optimized variant termed enCasδ. This variant enables robust genome editing in animal cells and plants, with overall editing performance comparable to that of SpCas9 and other Cas12 nucleases.
Fanghui Ge   +16 more
wiley   +1 more source

Enhancing CRISPR‐Cas12a base editing in plants with LbCas12a variants and introns

open access: yesJournal of Integrative Plant Biology, EarlyView.
Intron optimization of LbCas12a‐RRV improves cytosine and adenine base editing efficiency in plants, supports multiplexed and double‐strand break‐free genome modification, and expands precise genome engineering tools for crop breeding and plant functional research. ABSTRACT Cytosine base editors (CBEs) and adenine base editors (ABEs) are powerful tools
Yanhao Cheng   +5 more
wiley   +1 more source

Smart double‐screening system of propagating male‐sterile lines for maize hybrid seed production

open access: yesJournal of Integrative Plant Biology, EarlyView.
The Seed and Seedling Marker Method double‐screening system for maize hybrid seed production integrates Ms45‐mediated fertility restoration, Mn1/Sh1‐RNAi‐based seed selection, and Lc‐based seedling selection. It thus enables efficient, high‐purity propagation of male‐sterile lines by combining fertility maintenance with sequential visual selection at ...
Ruidan Liu   +10 more
wiley   +1 more source

Engineering glyphosate tolerance in sugarcane through overexpression of a mutated version of its native <i>EPSPS</i> gene. [PDF]

open access: yesGM Crops Food
Alves PI   +15 more
europepmc   +1 more source

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