Results 131 to 140 of about 348,200 (357)

Construction and Characterization of Novel Shuttle Expression Vectors for Actinobacillus succinogenes

open access: yesBiotechnology and Applied Biochemistry, EarlyView.
ABSTRACT Actinobacillus succinogenes is a ruminal microorganism of biotechnological importance due to its capacity to produce succinic acid at high yields. Despite the scientific interest in this organism, molecular vehicles for the transfer and expression of genetic material are limited compared to the existing demand.
Angélica Vallejo‐Giraldo   +7 more
wiley   +1 more source

Complex Interplay of Metabolic Substrates, Points of Entry into the Mitochondrial Electron Chain, and ROS Generation

open access: yesBioEssays, EarlyView.
Recently, Singh et al. studied the influence of reactive oxygen species (ROS) formation by the ETC on mitochondrial morphology in baker's yeast. Their findings highlight evolutionary constraints, important trade‐offs using different metabolic substrates, and connections with points of entry into the ETC, ROS production, the FADH2/NADH ratio concept ...
Dave Speijer
wiley   +1 more source

Scaling‐up the Bioconversion of Lignin to 2,4‐Pyridinedicarboxylic Acid With Engineered Pseudomonas putida for Bio‐Based Plastics Production

open access: yesBiotechnology and Bioengineering, EarlyView.
ABSTRACT 2,4‐pyridinedicarboxylic acid (PDCA) is a promising bio‐based compound to substitute petroleum‐derived terephthalic acid in plastics. It is produced through the microbial conversion of lignin substrates with engineered microorganisms like Pseudomonas putida.
Jan Seeger   +6 more
wiley   +1 more source

Engineering antibodies with cancer‐associated binding sites

open access: yesBMEMat, EarlyView.
This review describes antibodies that perform direct/indirect roles in cancer treatment by binding to effective targets and discusses how antibodies kill tumors and modulate tumor microenvironment through these targets. The classification of expression systems for antibody production is summarized to guide appropriate selection based on different ...
Yinqi Tian   +4 more
wiley   +1 more source

Enhancement of D‐mannitol production by fine‐tuned expression of mannitol‐1‐phosphate dehydrogenase in Synechocystis sp. PCC6803

open access: yesBiotechnology Progress, EarlyView.
Abstract D‐Mannitol production was achieved in freshwater Synechocystis sp. PCC6803 via the heterologous expression of mannitol‐1‐phosphate dehydrogenase (mtlD) and mannitol‐1‐phosphatase (m1p) under control of the strong promoter Ptrc1. However, only 5.54 mg L−1 of mannitol was found extracellularly after 7 days of cultivation, likely due to ...
Wenyang Wu   +3 more
wiley   +1 more source

Recombinant Expression and Bioprocess Optimization of Priestia megaterium α‐Amylase and Its Impact on Dough Fermentation Efficiency

open access: yesChemistry &Biodiversity, EarlyView.
This study presents a scalable strategy for producing Priestia megaterium α‐amylase (PmAmy) via fed‐batch fermentation, optimizing the biomass/IPTG ratio to enhance yield. With an activity of 67.7 U mL−1 at 22.3 g L−1 biomass, PmAmy demonstrated 99% efficiency in dough fermentation, highlighting its industrial potential as a sustainable baking enzyme .
Atacan Erdem   +3 more
wiley   +1 more source

Characterization of the Cubamyces Menziesii Terpenome

open access: yesChemBioChem, EarlyView.
The genome of Cubamyces menziesii reveals 18 putative sesquiterpene cyclase genes. These genes are cloned and expressed in Escherichia coli, yielding 10 active enzymes. Using farnesyl diphosphate as substrate, the enzymes are analyzed, and the products characterized after bioconversion, uncovering diverse sesquiterpene structures. This study highlights
Létitia Leydet   +10 more
wiley   +1 more source

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