Results 191 to 200 of about 261,803 (304)

Assessment of the phi6 lysis system using genetic complementation and heterologous expression in <i>Escherichia coli</i> and <i>Pseudomonas syringae</i>. [PDF]

Front Microbiol
Del Curto D, Zamora M, Lasley G, Khan R, Montalbano K, Ricken JB, Cahill J.   +6 more
europepmc   +1 more source

Microtubule Inner Protein CFAP77 Contributes to Sperm Motility and Male Fertility in Mice

Andrology, EarlyView.
ABSTRACT Background Sperm motility is essential for male fertility, and its regulation is dependent on the structural integrity of the axoneme. The axoneme consists of a conserved “9+2” microtubule arrangement and is supported by microtubule inner proteins. However, the functional significance of many microtubule inner proteins remains unclear.
Haoting Wang, Anh Hoang Pham, Mengjiao Luo, Masahito Ikawa, Haruhiko Miyata   +4 more
wiley   +1 more source

Heterologous expression of the M. abscessus CBASS system confers phage TM4 resistance in M. smegmatis. [PDF]

BMC Microbiol
Wang CL, Duan AF, Pan DM, Zhao YO, Yang J, Sun YC.   +5 more
europepmc   +1 more source

The Paediatric BCG Vaccine Century: From Historical Success to Future Innovations

Acta Paediatrica, EarlyView.
ABSTRACT Aim The tuberculosis (TB) vaccine, Bacille‐Calmette‐Guérin (BCG), protects infants against severe forms of primary TB. Yet, it does not protect against pulmonary reactivation TB in adults. Methods A literature review was conducted between 1st January and 28th February 2025 using PubMed, Google Scholar and reports of the World Health ...
Stefan H. E. Kaufmann
wiley   +1 more source

Heterologous expression of HCoV-HKU1 ORF 7b by mouse hepatitis virus protects against severe disease during murine infection. [PDF]

PLoS Pathog
Gunawardene CD, Pandey I, Chatterjee S, Peñaflor-Téllez Y, Odle A, Warren AN, Messyasz A, Rajsbaum R, Sariol A, Wong LR.   +9 more
europepmc   +1 more source

Tau‐targeting active immunotherapy slows progression and reduces pathology in mouse models of tauopathy

Brain Pathology, EarlyView.
The efficacy of the novel anti‐tau active immunotherapy, p5555kb, was tested using two mouse models of tau pathology. p5555kb inoculation increased the survival rate and reduced tau pathology in tau‐overexpressing P301L mice and decreased tau seeding in the brains of C57BL/6 mice injected with human‐purified Alzheimer's disease tau.
Christopher M. Brown, Jeanne K. Brooks, Louise Kelly, Madeline M. Vroom, Matthew Longo, Jean‐Cosme Dodart, Roxana Carare, Justin D. Boyd   +7 more
wiley   +1 more source

Mining of Novkitasetaline, a New Sulfur-Containing Antimalarial β-Carboline Alkaloid, from <i>Streptomyces</i> sp. PRh3 by Functional Ribosome Engineering Directed Heterologous Expression. [PDF]

Microorganisms
Chen X, He X, Wang Y, Feng Y, Wang Z, Song C, Yu X, Xie Y.   +7 more
europepmc   +1 more source

Rapid generation of prion disease models using AAV‐delivered PrP variants in knockout mice

Brain Pathology, EarlyView.
We developed a rapid AAV‐based system to generate prion disease models in weeks rather than months. Following systemic AAV9P31 delivery of modified PrP to knockout mice, we achieved brain‐wide expression and successful propagation of both classical (RML) and atypical (GSS‐A117V) prion strains.
Maitena San‐Juan‐Ansoleaga, Eva Fernández‐Muñoz, Jorge M. Charco, Enric Vidal, Diego Herrero‐Martínez, Josu Galarza‐Ahumada, Cristina Sampedro‐Torres‐Quevedo, Samanta Giler, Mariví Geijo, Gloria González‐Aseguinolaza, Hasier Eraña, Joaquín Castilla   +11 more
wiley   +1 more source

Identification of improved signal peptides for heterologous expression in Saccharomyces using a screen that exploits Gaussia luciferase. [PDF]

Sci Rep
Camboni G, Cartwright J, Grogan G.
europepmc   +1 more source

The L108I polymorphism in mouse prion protein drives spontaneous disease and enhances transmission of atypical and classical prion strains

Brain Pathology, EarlyView.
A single amino acid change (L108I) combined with PrP overexpression drives spontaneous atypical prion formation in mice, enabling also efficient propagation of diverse prion strains. This model allows studying how spontaneous prion diseases arise and provides powerful tools for investigating strain emergence, transmission barriers, and mechanisms ...
Hasier Eraña, Enric Vidal, Natalia Fernández‐Borges, Jorge M. Charco, Carlos M. Díaz‐Domínguez, Cristina Sampedro‐Torres‐Quevedo, Josu Galarza‐Ahumada, Eva Fernández‐Muñoz, Maitena San‐Juan‐Ansoleaga, Miguel Ángel Pérez‐Castro, Nuno Gonçalves‐Anjo, Patricia Piñeiro, Samanta Giler, Nora González‐Martín, Nuria L. Lorenzo, Africa Manero‐Azua, Guiomar Perez de Nanclares, Mariví Geijo, Manuel A. Sánchez‐Martín, Jesús R. Requena, Joaquín Castilla   +20 more
wiley   +1 more source