Results 211 to 220 of about 149,241 (263)
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Glycoprotein Preparation by Heterologous Expression
The eukaryotic expression, typically using cultured mammalian cells, is used for stable isotope labeling of glycoproteins. More recently, innovations have been made to reduce the cost of cell culture, control glycoforms, and employ more convenient expression systems other than mammalian cells.Koichi, Kato +2 more
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Heterologous protein expression in filamentous fungi
Trends in Biotechnology, 2005Filamentous fungi are commonly used in the fermentation industry for the large-scale production of proteins--mainly industrial enzymes. Recent advances in fungal genomics and related experimental technologies such as gene arrays and proteomics are rapidly changing the approaches to the development and use of filamentous fungi as hosts for the ...
K M Helena, Nevalainen +2 more
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Comprehensive Derivatization of Thioviridamides by Heterologous Expression
ACS Chemical Biology, 2019New technology for the derivatization of peptide natural products is required for drug development. Despite the recent advances in the genome sequencing technique enabling us to search for the biosynthetic genes for wide variety of natural products, the technical methods to get access to them are limited.
Kei Kudo +6 more
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Heterologous Expression of Peroxidases
2010The industrial importance of peroxidases has led to much research in the past two decades on the development of a cost effective and efficient production process for peroxidases. Unfortunately, even today, no clear answers can be given to questions such as (1) should the peroxidase be expressed in bacteria, yeast, or fungi?
Sandra de Weert, B. Christien Lokman
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Heterologous Protein Expression by Lactococcus lactis
2011This chapter describes the use of Lactococcus lactis as a safe and efficient cell factory to produce heterologous proteins of medical interest. The relevance of the use of this lactic acid bacterium (LAB) is that it is a noncolonizing, nonpathogenic microorganism that can be delivered in vivo at a mucosal level. The use of strains of L.
Villatoro-Hernández, J. +3 more
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Codon bias and heterologous protein expression
Trends in Biotechnology, 2004The expression of functional proteins in heterologous hosts is a cornerstone of modern biotechnology. Unfortunately, proteins are often difficult to express outside their original context. They might contain codons that are rarely used in the desired host, come from organisms that use non-canonical code or contain expression-limiting regulatory ...
Claes, Gustafsson +2 more
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Heterologous gene expression in Bacteroides fragilis
Plasmid, 1992Bacteroides fragilis and other gastrointestinal tract Bacteroides are unusual gram-negative eubacteria in that genes from other gram-negative eubacteria are not expressed when introduced into these organisms. To analyze gene expression in Bacteroides, expression vector and promoter probe (detection) vector systems were developed.
C J, Smith, M B, Rogers, M L, McKee
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Plasmids for heterologous expression in Pasteurella haemolytica
Gene, 1997New cloning and expression vectors that replicate both in Pasteurella haemolytica and in Escherichia coli were constructed based on a native sulfonamide (SuR) and streptomycin (SmR) resistant plasmid of P. haemolytica called pYFC1. Each shuttle vector includes an MCS and a selectable antibiotic resistance marker that is expressed in both organisms ...
N D, Fedorova, S K, Highlander
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Xenopus Oocytes as a Heterologous Expression System
20031. Introduct ion Oocytes of the South African clawed toad, Xenopus laevis, have proved a versatile and powerful heterologous expression system for eukaryotic genes (1). Xenopus oocytes were first used to express plant genetic material in 1979 (2), and m the past decade it has become increasingly evident that they are an appropriate and useful system to
F L, Theodoulou, A J, Miller
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Yeasts as a Tool for Heterologous Gene Expression
2011The yeasts Saccharomyces cerevisiae and Pichia pastoris are attractive hosts for production of human proteins. The main advantages offered by these systems are the well-developed and easily accessible genetic tools, rapid growth, the simple and inexpensive culture media, and many of the cellular and metabolic processes found in higher eukaryotes are ...
Raja, Mokdad-Gargouri +5 more
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