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The heterologous expression of H+ cotransport proteins in Xenopus oocytes
, 1993 Miller, A. J. +2 more
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Biochemical assessment of α-α-subunit interactions of Na v 1.5 in a heterologous expression system
Iamshanova O +7 more
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Errors of heterologous protein expression
Current Opinion in Biotechnology, 1996Missense substitutions and processivity errors in the translation of heterologous proteins are expected to occur at higher frequencies than the corresponding errors of normal translation. The resulting error-containing products may overload chaperone systems.
C, Kurland, J, Gallant
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Heterologous Expression and Purification of GPCRs
2022G protein-coupled receptors (GPCRs) are involved in a variety of human physiological processes and are attractive targets for treating various diseases. Yet, despite the importance as therapeutic targets, only 97 unique GPCR structures have been determined to date.
Xiaoting, Li +5 more
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Expression of heterologous proteins in Aspergillus
Journal of Biotechnology, 1991Filamentous fungi, in particular those of the genus Aspergillus have been well exploited for their ability to produce high levels of extracellular proteins in an inexpensive manner. Since many human proteins with the potential to be used therapeutically are secreted and require post-translational modification for biological activity, eukaryotic ...
M, Devchand, D I, Gwynne
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Heterologous Expression of Genes in Mycobacteria
2009Elucidating the function of mycobacterial proteins, determining their contribution to virulence, and developing new vaccine candidates has been facilitated by systems permitting the heterologous expression of genes in mycobacteria. Mycobacterium bovis bacille Calmette Guérin (BCG) and Mycobacterium smegmatis have commonly been employed as host systems ...
James A, Triccas, Anthony A, Ryan
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Glycoprotein Preparation by Heterologous Expression
The eukaryotic expression, typically using cultured mammalian cells, is used for stable isotope labeling of glycoproteins. More recently, innovations have been made to reduce the cost of cell culture, control glycoforms, and employ more convenient expression systems other than mammalian cells.Koichi, Kato +2 more
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Heterologous Expression of Peroxidases
2010The industrial importance of peroxidases has led to much research in the past two decades on the development of a cost effective and efficient production process for peroxidases. Unfortunately, even today, no clear answers can be given to questions such as (1) should the peroxidase be expressed in bacteria, yeast, or fungi?
Sandra de Weert, B. Christien Lokman
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Heterologous Protein Expression by Lactococcus lactis
2011This chapter describes the use of Lactococcus lactis as a safe and efficient cell factory to produce heterologous proteins of medical interest. The relevance of the use of this lactic acid bacterium (LAB) is that it is a noncolonizing, nonpathogenic microorganism that can be delivered in vivo at a mucosal level. The use of strains of L.
Villatoro-Hernández, J. +3 more
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Heterologous expression of calcium channels
The Journal of Membrane Biology, 1992J, Nargeot, N, Dascal, H A, Lester
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