Results 251 to 260 of about 261,803 (304)

Heterologous gene expression in Bacteroides fragilis

Plasmid, 1992
Bacteroides fragilis and other gastrointestinal tract Bacteroides are unusual gram-negative eubacteria in that genes from other gram-negative eubacteria are not expressed when introduced into these organisms. To analyze gene expression in Bacteroides, expression vector and promoter probe (detection) vector systems were developed.
C J, Smith, M B, Rogers, M L, McKee
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Heterologous Expression of Peroxidases

2010
The industrial importance of peroxidases has led to much research in the past two decades on the development of a cost effective and efficient production process for peroxidases. Unfortunately, even today, no clear answers can be given to questions such as (1) should the peroxidase be expressed in bacteria, yeast, or fungi?
Sandra de Weert, B. Christien Lokman
openaire   +1 more source

Heterologous Protein Expression by Lactococcus lactis

2011
This chapter describes the use of Lactococcus lactis as a safe and efficient cell factory to produce heterologous proteins of medical interest. The relevance of the use of this lactic acid bacterium (LAB) is that it is a noncolonizing, nonpathogenic microorganism that can be delivered in vivo at a mucosal level. The use of strains of L.
Villatoro-Hernández, J., Kuipers, O. P., Saucedo-Cárdenas, O., Montes-de-Oca-Luna, R.   +3 more
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Heterologous Expression of Genes in Mycobacteria

2009
Elucidating the function of mycobacterial proteins, determining their contribution to virulence, and developing new vaccine candidates has been facilitated by systems permitting the heterologous expression of genes in mycobacteria. Mycobacterium bovis bacille Calmette Guérin (BCG) and Mycobacterium smegmatis have commonly been employed as host systems ...
James A, Triccas, Anthony A, Ryan
openaire   +2 more sources

Heterologous Gene Expression in Filamentous Fungi

2012
Filamentous fungi are critical to production of many commercial enzymes and organic compounds. Fungal-based systems have several advantages over bacterial-based systems for protein production because high-level secretion of enzymes is a common trait of their decomposer lifestyle.
Xiaoyun, Su, George, Schmitz, Meiling, Zhang, Roderick I, Mackie, Isaac K O, Cann   +4 more
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Sarcoglycan heterologous expression.

2004
Mutations in sarcoglycans (SGs) cause autosomal-recessive limb-girdle muscular dystrophy. SGs exist as a complex of four transmembrane proteins (a, b, c, and d). We have recently shown that a-SG is an ecto-ATPase, i.e. an enzyme able to hydrolyze extracellular ATP.
GASTALDELLO S, SANDONA', DORIANNA, MADDALONI C, DANGELO S, FRASSON I, RUBERTO I, SANTAMARIA L, MARTINELLO, TIZIANA, CHAN Y, BETTO R.   +9 more
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Understanding bacteriocin heterologous expression: A review

International Journal of Biological Macromolecules
Bacteriocins are a diverse group of ribosomally synthesised antimicrobial peptides/proteins that play an important role in self-defence. They are widely used as bio-preservatives and effective substitutes for disease eradication. They can be used in conjunction with or as an alternative to antibiotics to minimize the risk of resistance development ...
Priyanshi M, Jain, Anagha, Nellikka, Rajagopal, Kammara   +2 more
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Glucose Uptake in Heterologous Expression Systems

2017
Understanding the physiological regulation of glucose transport requires the analysis of transporters, like GLUT1, in diverse tissue types. We document the utility of viral vectors for the stable expression of wild-type and modified GLUT1 transporter in different types of mammalian cells.
Eunice E, Lee, Richard C, Wang
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Expression of Heterologous Genes

1991
With the advent of recombinant DNA technology in the mid-1970s a number of shrewd academics quickly realized the commercial potential of these tools in producing proteins of high commercial value, which, until that time, had proven both difficult and expensive to isolate in quantities sufficient for clinical trials.
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Metabolic load and heterologous gene expression

Biotechnology Advances, 1995
The expression of a foreign protein(s) in a recombinant host cell or organism often utilizes a significant amount of the host cell's resources, removing those resources away from host cell metabolism and placing a metabolic load (metabolic drain, metabolic burden) on the host.
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