Results 71 to 80 of about 261,803 (304)

Heterologous expression of heterotrophic nitrification genes [PDF]

Microbiology, 1997
Paracoccus denitrificans is a heterotrophic organism capable of oxidizing ammonia to nitrite during growth on an organic carbon and energy source. This pathway, termed heterotrophic nitrification, requires the concerted action of an ammonia monooxygenase (AMO) and hydroxylamine oxidase (HAO).
Crossman, Lisa C., Moir, James W.B., Enticknap, Julie J., Richardson, David J., Spiro, Stephen   +4 more
openaire   +3 more sources

Bacteria and Fungi Synergistically Reprogram Flavonoid Metabolites in the Pericarp of Citrus Reticulata 'Chachi' During Storage

Advanced Science, EarlyView.
The authors employed widely targeted metabolomics, microbial amplicon sequencing, and fermentation assays to investigate the microbiome 's influence on PCRC 's (pericarp of Citrus reticulata ' Chachi ') flavonoid profile over 0–19 years of storage. Based on the correlation analysis, solid‐state and liquid‐state fermentation, and catalytic activity ...
Jianmu Su, Sisheng Zhang, Lidi Liang, Haiyi Bai, Mei Bai, Hanjun He, Yayu Wang, Huan Liu, Xiangxiu Liang, Yu Sun, Hong Wu   +10 more
wiley   +1 more source

Heterologous protein expression is enhanced by harmonizing the codon usage frequencies of the target gene with those of the expression host. [PDF]

PLoS ONE, 2008
Synonymous codon replacement can change protein structure and function, indicating that protein structure depends on DNA sequence. During heterologous protein expression, low expression or formation of insoluble aggregates may be attributable to ...
Evelina Angov, Collette J Hillier, Randall L Kincaid, Jeffrey A Lyon   +3 more
doaj   +1 more source

Engineering of Burkholderia thailandensis strain E264 serves as a chassis for expression of complex specialized metabolites

Frontiers in Microbiology, 2022
Heterologous expression is an indispensable approach to exploiting natural products from phylogenetically diverse microbial communities. In this study, we constructed a heterologous expression system based on strain Burkholderia thailandensis E264 by ...
Zong-Jie Wang, Xiaotong Liu, Haibo Zhou, Yang Liu, Lin Zhong, Lin Zhong, Xue Wang, Qiang Tu, Qiang Tu, Liujie Huo, Fu Yan, Lichuan Gu, Rolf Müller, Youming Zhang, Youming Zhang, Xiaoying Bian, Xiaokun Xu   +16 more
doaj   +1 more source

Heterologous prime-boost vaccination with H3N2 influenza viruses of swine favors cross-clade antibody responses and protection [PDF]

, 2017
The emergence of multiple novel lineages of H1 and H3 influenza A viruses in swine has confounded control by inactivated vaccines. Because of substantial genetic and geographic heterogeneity among circulating swine influenza viruses, one vaccine strain ...
Claes, Gerwin, Cox, Eric, Krammer, Florian, Mancera Gracia, José Carlos, Qiu, Yu, Sys, Lieve, Trus, Ivan, Van Reeth, Kristien, Versnaeyen, Han   +8 more
core   +2 more sources

Expression of Lectins in Heterologous Systems [PDF]

International Journal of Molecular Sciences, 2018
Lectins are proteins that have the ability to recognize and bind in a reversible and specific way to free carbohydrates or glycoconjugates of cell membranes. For these reasons, they have been extensively used in a wide range of industrial and pharmacological applications.
Dania Martínez-Alarcón, Alejandro Blanco-Labra, Teresa García-Gasca   +2 more
openaire   +2 more sources

Pre‐Encoded IFN‐I Sensitivity Exacerbates Memory T Cell Senescence in Solid Tumors

Advanced Science, EarlyView.
Type I interferon (IFN‐I) signaling promotes p21‐dependent cell cycle arrest in senescent tumor‐specific memory T cells, resulting in poor proliferative responses and solid tumor regression during cancer vaccination. Conversely, IFNα/β receptor blockade reinvigorates T cell proliferation to regress solid tumors and is more effective with increasing ...
Andrew Nguyen, Scott R Walsh, Li Deng, Lan Chen, Yonghong Wan   +4 more
wiley   +1 more source

Chromatin Topology Reconfiguration Orchestrates Thermotolerant Male Fertility via GhAL5 in Cotton

Advanced Science, EarlyView.
This study investigates cotton’s high‐temperature (HT) response using multi‑omics. Dynamic 3D genome changes drive expression bias affecting male fertility. The tolerant line shows controlled chromatin dynamics, while the sensitive line exhibits overactivation.
Yanlong Li, Jing Yang, Weiran Wang, Chunyang Zuo, Liuling Pei, Yizan Ma, Rui Zhang, Yaru Fan, Huanhuan Ma, Yawei Li, Ruizhen Liu, Shuangxia Jin, Longfu Zhu, Jie Kong, Xianlong Zhang, Ling Min   +15 more
wiley   +1 more source

Novel Nonreplicating Vaccinia Virus Vector Enhances Expression of Heterologous Genes and Suppresses Synthesis of Endogenous Viral Proteins

mBio, 2017
Viruses are used as expression vectors for protein synthesis, immunology research, vaccines, and therapeutics. Advantages of poxvirus vectors include the accommodation of large amounts of heterologous DNA, the presence of a cytoplasmic site of ...
Linda S. Wyatt, Wei Xiao, Jeffrey L. Americo, Patricia L. Earl, Bernard Moss   +4 more
doaj   +1 more source

Heterologous Expression of the Class IIa Bacteriocins, Plantaricin 423 and Mundticin ST4SA, in Escherichia coli Using Green Fluorescent Protein as a Fusion Partner

Frontiers in Microbiology, 2020
The antilisterial class IIa bacteriocins, plantaricin 423 and mundticin ST4SA, have previously been purified from the cell-free supernatants of Lactobacillus plantarum 423 and Enterococcus mundtii ST4SA, respectively.
Ross Rayne Vermeulen, Anton Du Preez Van Staden, Anton Du Preez Van Staden, Leon Dicks   +3 more
doaj   +1 more source