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Determination of hexosamines

Archives of Biochemistry and Biophysics, 1961
Abstract A simple method for the colorimetric determination of hexosamines is presented. After deamination of the hexosamine with nitrous acid, phenol-sulfuric acid reagents are added and the absorbancy is measured at 490 mμ. Interfering substances are removed previously by ion-exchange procedures.
Y C, LEE, R, MONTGOMERY
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Hexosamines and Insulin Resistance

Diabetes, 1996
Glucose is an important regulator of cell growth and metabolism. Thus, it is likely that some of the adverse effects of hyperglycemia are reflections of normal regulation by abnormal concentrations of glucose. How the cell senses glucose, however, is still incompletely understood.
D A, McClain, E D, Crook
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Urinary hexosamine and acetyl-hexosamine excretion in human acetylator phenotypes

Clinica Chimica Acta, 1967
Abstract Human subjects are divisible into two clearly defined phenotypes by their capacity to acetylate sulfamethazine. The biochemical basis of this polymorphism lies in the possession of high N-acetyltransferase activity in the liver and intestinal mucosa of rapid acetylators as compared with slow acetylators.
T A, White, D A, Evans
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Spectrophotometric assay for hexosamines

Analytical Biochemistry, 1966
Abstract A new, simple, and sensitive method was described for the determination of hexosamines based on color development with trinitrobenzene sulfonate (TNBS). The absorbancy of the trinitrophenyl (TNP)-hexosamines was proportional to concentration over a 200-fold range.
J T, Galambos, R, Shapira
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Colorimetric differentiation of hexosamines

Analytical Biochemistry, 1967
Abstract A colorimetric method is introduced to differentiate glucosamine, galactosamine, and mannosamine. Direct evaluation of galactosamine in mixture with glucosamine was possible since galactosamine was essentially the only component responsible for the color of the reaction; the influence of glucosamine on the intensity of the color was ...
J, Ludowieg, J D, Benmaman
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Hexosamine in Bombyx mori Silk

Nature, 1965
CONSTITUENTS other than protein in Bombyx mori silk have received little attention1. The significant amounts of hexosamine found in hair and wool2 prompted this study to quantitate and to identify the amino-sugars in B. mori silk.
J G, Smith, R D, Clark
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d-Hexosaminate production by oxidative fermentation

Applied Microbiology and Biotechnology, 2004
Microbial production of D-hexosaminate was examined by means of oxidative fermentation with acetic acid bacteria. In most strains of acetic acid bacteria, membrane-bound D-glucosamine dehydrogenase (synonymous with an alternative D-glucose dehydrogenase distinct from quinoprotein D-glucose dehydrogenase) oxidized D-hexosamines to the corresponding D ...
D, Moonmangmee   +3 more
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Determination of hexosamines by gas-liquid chromatography

Analytical Biochemistry, 1966
Abstract Gas-liquid chromatography (GLC) has provided a powerful technique for the analysis of several types of compounds, e.g., steroids, fatty acids, and carbohydrates. Since GLC of free carbohydrates is limited because of low volatility of the compounds, several volatile derivatives-methyl, ethyl, and trimethylsilyl (TMS)—have been used in GLC ...
B, Radhakrishnamurthy   +2 more
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