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High-Performance Liquid Chromatography
2001Chromatography is a general term applied to a wide variety of separation techniques based on the sample partitioning between a moving phase, which can be a gas, liquid, or supercritical fluid, and a stationary phase, which may be either a liquid or a solid.
John G. Dorsey, Thomas H. Stout
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High-performance liquid chromatography of porphyrins
Journal of Chromatography B: Biomedical Sciences and Applications, 1988Techniques for the analysis of porphyrins in the biomedical fields are reviewed. The emphasis is on high-performance liquid chromatography and its aspplications in: (1) the quantitative analysis of porphyrins in blood, urine and faeces; (2) qualitative porphyrin profiles in normal subjects and in the porphyrias; (3) assay of haem biosynthetic enzyme ...
T.J. Peters, Famei Li, Chang Kee Lim
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High performance “ionic liquid” chromatography
Chemical Communications, 2011We firstly developed high performance liquid chromatography methods with "ionic liquids" as eluents (HPILC) and successfully demonstrated the analysis of various biopolymers including scarcely soluble highly polymerized cellulose by means of HPILC with highly polar ionic liquid.
Hiroyuki Ohno+3 more
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High-performance liquid chromatography of insulin
Journal of Chromatography A, 1984The elution behaviour of a selection of sequence variant and modified insulins has been compared on a C18 reversed-phase column. Observed elution times were compared with those expected from the nature of the differences from bovine insulin. In some cases prediction rules established for peptides are adequate to explain the observed elution and in ...
S.P. Wood, A. McLeod
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High-Performance Liquid Chromatography
1988Considerable attention has recently been focused on the biosynthesis and metabolism of bile acids in patients with hepatobiliary diseases, and the development of reliable methods is urgently required for the analysis of profiles of bile acids in biological materials.
Junichi Goto, Toshio Nambara
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High-performance liquid chromatography of cardiolipin
Journal of Chromatography B: Biomedical Sciences and Applications, 1985Resolution of freshly prepared and of commercially available (degraded) samples of cardiolipin into 15-30 components has been accomplished by reversed-phase high-performance liquid chromatography using a 3-micron particulate Microsorb C18 column irrigated with linear gradients of acetonitrile--methanol--10 mM phosphate buffer pH 7.4.
Leland L. Smith, Jon I. Teng
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High-performance liquid chromatography of isopeptides
Journal of Chromatography A, 1986High-performance liquid chromatographic (HPLC) methods are described for the structural analysis of clavicepamines, their analogues and branched-chain polypeptides, the analysis in synthetic stages of isopeptides (analysis of half-protected derivatives and purity control of active esters) and the differentiation between alpha- and iso-peptides (such as
Gabriella Kelemen+2 more
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High Performance Liquid Chromatography
2019High performance liquid chromatography (HPLC) is a form of liquid chromatography which is used to separate the individual components of interest present in mixture and/or dissolved in sample solution. It is based on pumping of mobile phase through the packed column under high pressure.
Muhammad Sajid Hamid Akash+1 more
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High-performance liquid chromatography of proteins
Journal of Chromatography A, 1976Proteins were separated on microparticulate bonded phase steric exclusion and anion-exchange chromatography supports. A post-column enzyme detector was developed which gives a specific and sensitive response for enzymes. The three iso-enzymes of creatine phosphokinase were separated and assayed in 4 min and the five isoenzymes of lactic dehydrogenase ...
Karen M. Gooding+2 more
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High performance liquid chromatography
1999The pioneering work of Martin and Synge [1] led to the development of gas-liquid chromatography, an elegant chromatographic method where, even with packed columns, the efficiencies achieved were high and the technique could be used with equal facility for both analytical and preparative studies.
F. J. Smith, A. Braithwaite
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