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High-performance liquid chromatography of proteins

Journal of Chromatography A, 1976
Proteins were separated on microparticulate bonded phase steric exclusion and anion-exchange chromatography supports. A post-column enzyme detector was developed which gives a specific and sensitive response for enzymes. The three iso-enzymes of creatine phosphokinase were separated and assayed in 4 min and the five isoenzymes of lactic dehydrogenase ...
Shung Ho Chang   +2 more
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High-performance liquid chromatography of porphyrins

Journal of Chromatography B: Biomedical Sciences and Applications, 1988
Techniques for the analysis of porphyrins in the biomedical fields are reviewed. The emphasis is on high-performance liquid chromatography and its aspplications in: (1) the quantitative analysis of porphyrins in blood, urine and faeces; (2) qualitative porphyrin profiles in normal subjects and in the porphyrias; (3) assay of haem biosynthetic enzyme ...
C K, Lim, F M, Li, T J, Peters
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High-Performance Liquid Chromatography of Cisplatin

Journal of Pharmaceutical Sciences, 1983
The retention behavior of cisplatin on a variety of stationary phases has been investigated using aqueous mobile phases modified by the addition of various electrolytes and methanol. Cisplatin is poorly retained on reverse-phase or silica columns but satisfactorily retained on chemically bonded or solvent-generated anion exchangers.
C M, Riley, L A, Sternson, A J, Repta
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High-performance liquid magneto-chromatography

Journal of Chromatography A, 2006
High-performance liquid magneto-chromatography (HPLMC) is a new chromatography technique with two distinctive features: (a) a high surface area stationary phase with paramagnetic properties composed of magnetite embedded in a silica gel, and (b) a magnetic field (variable intensity 0-5.5 mT) that selectively retains paramagnetic substances in the ...
E, Barrado, J A, Rodríguez
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High-performance liquid chromatography of tetracyclines

Journal of Chromatography A, 1986
The possibilities of two non-ionogenic resins (XAD-2 and PRP-1) in the chromatography of tetracyclines and their degradation products are described. It appears that, in contrast to silica-based materials, this type of column material produces linear calibration curves, even in the low nanogram range.
H J, Reeuwijk, U R, Tjaden
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High-performance liquid chromatography of antibiotics

Journal of Chromatography B: Biomedical Sciences and Applications, 1990
High-performance liquid chromatographic (HPLC) monitoring of antimicrobial agents has recently become more widely used, and represents an interesting alternative to other methods. The methodology is characterized by good specificity and accuracy, and it is applicable to almost all antibiotics.
F, Jehl, C, Gallion, H, Monteil
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High-performance liquid-liquid chromatography

Journal of Chromatography A, 1984
Abstract The applicability of liquid-liquid systems as phase systems in high-performance liquid chromatography (HPLC) was investigated. The more polar phase of the liquid-liquid system is generated in situ on pre-packed silica columns by pumping through the saturated mobile phase.
J.P. Crombeen, S. Heemstra, J.C. Kraak
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High-Performance Liquid Chromatography of Biopolymers

Science, 1983
The ability to separate biological macromolecules with good resolution on liquid chromatographic columns has depended on the development of suitable packing materials. In size exclusion chromatography, molecules are separated by size on the basis of differential permeation of the packing.
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Chromatography: High-Performance Liquid Chromatography

2016
High-performance liquid chromatography (HPLC) is a major analytic tool in contemporary science, with possibly the highest number of systems installed and running globally. Modern HPLC offers high resolutions allowing the quantitative determination of target analytes within complex matrices by its compatibility with a number of detectors.
Gika, Helen   +3 more
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High Performance Liquid Chromatography

2003
For those new to this technique, this guide provides basic tips, key skills, awareness and guidance on good practice of High Performance Liquid Chromatography (HPLC). It will help build understanding of the important issues to consider during analysis and how to develop further skills.
Brian Stuart, Elizabeth Prichard
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