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Size Exclusion-High-Performance Liquid Chromatography (SEC-HPLC)
2014The size exclusion-high-performance liquid chromatography is a high-throughput analytical method, through isocratic condition, that allows to determine and quantify the level of aggregates and fragments of purified antibodies. Here, we describe the preparation of the samples, chromatographic conditions to be used (buffer preparation, quantity injected,
Delphine, Schrag +2 more
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Ion Exchange-High-Performance Liquid Chromatography (IEX-HPLC)
2014The ion exchange-high-performance liquid chromatography is a high-throughput analytical method that allows to determine the charge profile of purified antibodies. Here, we describe the preparation of the samples, chromatographic conditions to be used (buffer preparation, salt gradient, quantity injected, flow rate, run time, column suitability, etc ...
Marie, Corbier +2 more
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Purification of Monoclonal Antibodies Using High Performance Liquid Chromatography (HPLC)
Preparative Biochemistry, 1984Recent increases in the ability to detect low levels of immunofluorescence have shown the need for highly purified primary immunoreagents. There are now reports of purification of monoclonal antibodies using HPLC with reverse phase columns. In this study we have utilized standard size exclusion HPLC to purify both biotinylated and non-biotinylated ...
G A, Perry +4 more
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Chiral High-Performance Liquid Chromatography (HPLC) in Targeted Metabolomics
Enzymatic reactions usually occur with a high substrate, product, or substrate-product stereoselectivity. In many instances, information on the stereochemical disposition of biologically active metabolites is indispensable for a complete understanding of biological processes and metabolic states.Xiaoqing, Fu, Michael, Lämmerhofer
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HPLC: high performance liquid chromatography
Environmental Science & Technology, 1979Michael Lynch, Ed Weiner
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[Determination of sarafloxacin by high performance liquid chromatography (HPLC)].
Se pu = Chinese journal of chromatography, 2003A method for the separation and determination of Sarafloxacin by HPLC was developed. The sample was dissolved in a mixture of V(acetonitrile):V(water) = 1:1. Operating conditions were as follows: mu-Bondapak C18 column (3.9 mm x 300 mm), V(acetonitrile):V(methanol):V(2 mmol/L H3PO4, adjust pH 3.5 with triethylamine) = 30:5:65 as mobile phase with a ...
J, He +5 more
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High-performance liquid chromatography (HPLC)
Proceedings of the Analytical Division of the Chemical Society, 1975R. E. Majors +2 more
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