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A Review on High Performance Liquid Chromatography (HPLC)

Asian Journal of Pharmaceutical Analysis, 2023
The estimate of pharmaceutical and biological materials typically uses the key qualitative and quantitative technique known as high performance liquid chromatography (HPLC). It is the most adaptable, secure, dependable, and quick chromatographic method for determining the quality of medicinal ingredients.
Sahil Kamble, Sahil Agrawal, Sagar Pagade, Rahul Patil, Nilesh Chaugule, Anuja Patil   +5 more
openaire   +1 more source

High Performance Liquid Chromatography (HPLC)

1985
Liquid chromatography (LC) is the generic name used to describe any chromatographic procedure in which the mobile phase is a liquid. Techniques already discussed which belong to this general classification are paper chromatography, thin-layer chromatography (TLC) and variants of traditional column chromatography.
A. Braithwaite, F. J. Smith
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Size Exclusion-High-Performance Liquid Chromatography (SEC-HPLC)

2014
The size exclusion-high-performance liquid chromatography is a high-throughput analytical method, through isocratic condition, that allows to determine and quantify the level of aggregates and fragments of purified antibodies. Here, we describe the preparation of the samples, chromatographic conditions to be used (buffer preparation, quantity injected,
Delphine, Schrag, Marie, Corbier, Sylvain, Raimondi   +2 more
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Ion Exchange-High-Performance Liquid Chromatography (IEX-HPLC)

2014
The ion exchange-high-performance liquid chromatography is a high-throughput analytical method that allows to determine the charge profile of purified antibodies. Here, we describe the preparation of the samples, chromatographic conditions to be used (buffer preparation, salt gradient, quantity injected, flow rate, run time, column suitability, etc ...
Marie, Corbier, Delphine, Schrag, Sylvain, Raimondi   +2 more
openaire   +2 more sources

High Performance Liquid Chromatography (HPLC)

1992
The general principles of liquid chromatography were first outlined in 1903 by the botanist Tswett in his description of adsorption chromatography for the separation of natural pigments, such as chlorophylls (Tswett 1903). Since then remarkable progress has been made, as evidenced by the multitude of techniques employed today in thin layer, low ...
openaire   +1 more source

Separation of Hemoglobin Peptides by high Performance Liquid Chromatography (HPLC)

Hemoglobin, 1980
(1980). Separation of Hemoglobin Peptides by high Performance Liquid Chromatography (HPLC) Hemoglobin: Vol. 4, No. 3-4, pp. 551-559.
W A, Xchroeder, J B, Shelton, J R, Shelton   +2 more
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Purification of Monoclonal Antibodies Using High Performance Liquid Chromatography (HPLC)

Preparative Biochemistry, 1984
Recent increases in the ability to detect low levels of immunofluorescence have shown the need for highly purified primary immunoreagents. There are now reports of purification of monoclonal antibodies using HPLC with reverse phase columns. In this study we have utilized standard size exclusion HPLC to purify both biotinylated and non-biotinylated ...
G A, Perry, J D, Jackson, T L, McDonald, D A, Crouse, J G, Sharp   +4 more
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Miniaturization Of High Performance Liquid Chromatography (Micro-Hplc)

1985
Publisher Summary This chapter discusses the miniaturization of high performance liquid chromatography. Microbore systems, Capillary LC, High Speed Liquid Chromatography (HSLC) , Low Dispersion Liquid Chromatography (LDLC) are new developments which have received much attention in the last years.
M. Verzele, C. Dewaele
openaire   +1 more source