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High-speed countercurrent chromatography
Nature, 1987Support-free high-speed countercurrent liquid chromatography provides a rich domain of applications, some beyond reach of conventional liquid chromatography.
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Mass transfer and separation criteria for high‐speed countercurrent chromatography
AIChE Journal, 2011AbstractHigh‐speed countercurrent chromatography (HSCCC) is a versatile technique for preparative separations of a wide variety of solutes. For optimization of operating conditions, prediction of separations, and scale‐up study, a model is needed to describe the effluent concentration profile, which determines the separation efficiency (mass transfer ...
Zhao, Chun-Xia, He, Chao-Hong
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Journal of Agricultural and Food Chemistry, 2008
Curcuminoids are substances of great interest because of their important pharmacological activities, particularly anti-inflammatory, anticarcinogenic, and anti-Alzheimer's activities. In this study, we report the first procedure and effect of processing for the high, efficient, and useful purification of curcumin, demethoxycurcumin, and ...
Koichi, Inoue +5 more
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Curcuminoids are substances of great interest because of their important pharmacological activities, particularly anti-inflammatory, anticarcinogenic, and anti-Alzheimer's activities. In this study, we report the first procedure and effect of processing for the high, efficient, and useful purification of curcumin, demethoxycurcumin, and ...
Koichi, Inoue +5 more
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Concentration of Heavy Metals by High-Speed Countercurrent Chromatography
Journal of Liquid Chromatography & Related Technologies, 1998Application of high-speed countercurrent chromatography (HSCCC) to the concentration of metal ions has been studied. The stationary phase, containing di(2-ethylhexyl) phosphoric acid (DEHPA) in n-heptane, was continuously fed into the column. The ppb level of metal ions in a 500 mL of the mobile phase was continuously concentrated into small volumes of
E. Kitazume, N. Sato, Y. Ito
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Separation of heavy metals by high-speed countercurrent chromatography
Analytical Chemistry, 1993Separation of heavy metal elements such as Co, Cu, Fe(II),Fe(III), Mg, and Ni was performed with a high-speed CCC coil planet centrifuge equipped with a multilayer coil. The two-phase solvent systems used for the separation were composed of n-heptane containing bis(2-ethylhexyl) phosphoric acid (stationary phase) and diluted citric acid (mobile phase),
Eiichi. Kitazume +3 more
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Preparative Separation of Polyphenols from Tea by High-Speed Countercurrent Chromatography
Journal of Agricultural and Food Chemistry, 2000High-speed countercurrent chromatography (HSCCC) was applied to the separation of polyphenols from tea leaves (Camellia sinensis L.). The capability of HSCCC to isolate pure tea polyphenols from complex mixtures on a preparative scale was demonstrated for catechins, flavonol glycosides, proanthocyanidins, and strictinin from green and black tea.
A, Degenhardt +3 more
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Current Pharmaceutical Biotechnology, 2015
Achyrobichalcone is a biflavonoid recently found in Achyrocline satureioides. This substance has unprecedented chemical structure and occurrence, but resembles other bioactive bichalcones, which have important pharmacological properties, such as anticancer activity.
Juliana P, Carini +7 more
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Achyrobichalcone is a biflavonoid recently found in Achyrocline satureioides. This substance has unprecedented chemical structure and occurrence, but resembles other bioactive bichalcones, which have important pharmacological properties, such as anticancer activity.
Juliana P, Carini +7 more
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Journal of Chromatography B, 2008
High-speed countercurrent chromatography (HSCCC) has been applied for the separation of theaflavins and catechins. The HSCCC run was carried out with a two-phase solvent system composed of hexane-ethyl acetate-methanol-water-acetic acid (1:5:1:5:0.25, v/v) by eluting the lower aqueous phase at 2 ml/min at 700 rpm.
Kunbo, Wang +6 more
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High-speed countercurrent chromatography (HSCCC) has been applied for the separation of theaflavins and catechins. The HSCCC run was carried out with a two-phase solvent system composed of hexane-ethyl acetate-methanol-water-acetic acid (1:5:1:5:0.25, v/v) by eluting the lower aqueous phase at 2 ml/min at 700 rpm.
Kunbo, Wang +6 more
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Characterization of Pigments from Different High Speed Countercurrent Chromatography Wine Fractions
Journal of Agricultural and Food Chemistry, 2005A red wine, made from Cabernet Sauvignon (60%) and Tannat (40%) cultivars, was fractionated by high speed countercurrent chromatography (HSCCC). The biphasic solvent system consisting of tert-butyl methyl ether/n-butanol/acetonitrile/water (2/2/1/5, acidified with 0.1% trifluoroacetic acid) was chosen for its demonstrated efficiency in separating ...
Salas, Erika +5 more
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HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY USING A SMALL COILED COLUMN
Journal of Liquid Chromatography & Related Technologies, 2000Separation of selected lanthanoid elements was performed by high-speed countercurrent chromatography (CCC) equipped with a small coiled column of a 10 cm3 capacity. A hexane solution of 2-ethylhexylphosphonic acid mono-2-ethylhexyl ester (EHPA) was retained in the tubing column as the stationary phase.
Akira Tsuyoshi +4 more
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