Results 81 to 90 of about 373,405 (315)

High-Throughput Sequencing Reveals Bacterial Diversity in Raw Milk Production Environment and Production Chain in Tangshan City of China

Food Science of Animal Resources, 2021
Raw milk is a nature media of microbiota that access milk from various sources, which constitutes a challenge in dairy production. This study characterizes the relationship between the raw milk quality and the bacteria diversity at different sampling ...
Huihui Cao, Yanhua Yan, Lei Wang, Lixue Dong, Xueliang Pang, Sining Tang, Aijun Li, Aili Xiang, Litian Zhang, Baiqin Zheng   +9 more
doaj   +1 more source

Hyb:A bioinformatics pipeline for the analysis of CLASH (crosslinking, ligation and sequencing of hybrids) data [PDF]

, 2013
Peer reviewedPublisher ...
Aiba, Aleksandra Helwak, Altschul, Anthony J. Travis, Aravin, Chi, Chi, David Tollervey, Dodt, Field, Filipowicz, Gong, Granneman, Grzegorz Kudla, Hafner, Hansen, Helwak, Jonathan Moody, Kent, Kim, Kudla, Langmead, Leung, Licatalosi, Lorenz, Markham, Memczak, Quinlan, Rost, Simpson, Ule, Wlotzka, Zhang   +32 more
core   +2 more sources

Upscaling the surveillance of tick-borne pathogens in the French Caribbean Islands [PDF]

, 2020
Despite the high burden of vector-borne disease in (sub)tropical areas, few information are available regarding the diversity of tick and tick-borne pathogens circulating in the Caribbean.
Albina, Emmanuel, Aprelon, Rosalie, Delannoy, Sabine, Devillers, Elodie, Galon, Clémence, Gondard, Mathilde, Moutailler, Sara, Pinarello, Valérie, Pradel, Jennifer, Vayssier-Taussat, Muriel   +9 more
core   +3 more sources

High-throughput targeted long-read single cell sequencing reveals the clonal and transcriptional landscape of lymphocytes

Nature Communications, 2018
High-throughput single-cell RNA sequencing is a powerful technique but only generates short reads from one end of a cDNA template, limiting the reconstruction of highly diverse sequences such as antigen receptors. To overcome this limitation, we combined
Mandeep Singh, G. Al-Eryani, Shaun Carswell, James M. Ferguson, J. Blackburn, K. Barton, D. Roden, F. Luciani, Tri Giang Phan, S. Junankar, Katherine J. L. Jackson, C. Goodnow, Martin A. Smith, A. Swarbrick   +13 more
semanticscholar   +1 more source

Transcriptional profiling of circulating extracellular vesicles from prebiopsy prostate cancer patients

Molecular Oncology, EarlyView.
RNA profiling of circulating extracellular vesicles (EVs) from blood samples of men undergoing prostate biopsy identifies transcripts associated with clinically significant prostate cancer. Integrative analysis with public tumor datasets links EV‐derived gene signatures to tumor stage and progression‐free survival, highlighting CASP3, XRCC2, and RIT1 ...
Stefan Werner, Pierre Tennstedt, Randi C. Pose, Christian Müller, Katharina Besler, Svenja Schneegans, Malik Alawi, Marie C. Roesch, Sven Peine, Desiree Bonci, Joanna Budna‐Tukan, Evi Lianidou, Catherine Alix‐Panabières, Derya Tilki, Klaus Pantel   +14 more
wiley   +1 more source

Joint Haplotype Assembly and Genotype Calling via Sequential Monte Carlo Algorithm [PDF]

, 2015
Genetic variations predispose individuals to hereditary diseases, play important role in the development of complex diseases, and impact drug metabolism. The full information about the DNA variations in the genome of an individual is given by haplotypes,
Ahn, Soyeon, Vikalo, Haris
core   +3 more sources

Longitudinal circulating tumor DNA profiling in patients with advanced endometrial cancer using an off‐the‐shelf targeted NGS panel

Molecular Oncology, EarlyView.
Intratumour heterogeneity complicates precision management of advanced endometrial cancer. Circulating tumor DNA (ctDNA) offers a minimally invasive strategy to capture tumor evolution and therapeutic resistance. Here, we compare tumor‐agnostic NGS with tumor‐informed ddPCR, outlining their relative sensitivity, concordance, and clinical implications ...
Carlos Casas‐Arozamena, Karin Teien Lande, Eva Diaz, Ana Vilar, Juan Cueva, Efigenia Arias, Victoria Sampayo, Alicia Abalo, Nerea González, Eva Colás, Antonio Gil‐Moreno, Miguel Abal, Gema Moreno‐Bueno, Therese Sørlie, Kristina Lindemann, Laura Muinelo‐Romay   +15 more
wiley   +1 more source

Additional file 2 of Correcting nucleotide-specific biases in high-throughput sequencing data

, 2017
Table S1. Area under curve (AUC) values for the ROC curves representing sensitivity and specificity of footprint detection for several transcription factors. AUC values at increasing false positive rates (FPR) are computed independently for each motif before and after correction.
Wang, Jeremy, Quach, Bryan, Furey, Terrence   +2 more
openaire   +1 more source

High, clustered, nucleotide diversity in the genome of Anopheles gambiae revealed through pooled-template sequencing: implications for high-throughput genotyping protocols [PDF]

BMC Genomics, 2009
Association mapping approaches are dependent upon discovery and validation of single nucleotide polymorphisms (SNPs). To further association studies in Anopheles gambiae we conducted a major resequencing programme, primarily targeting regions within or close to candidate genes for insecticide resistance.Using two pools of mosquito template DNA we ...
Steen Keith, Weetman David, Wilding Craig S, Donnelly Martin J   +3 more
openaire   +4 more sources

Experience of copy number variation sequencing applied in spontaneous abortion

BMC Medical Genomics
Purpose We evaluated the value of copy number variation sequencing (CNV-seq) and quantitative fluorescence (QF)-PCR for analyzing chromosomal abnormalities (CA) in spontaneous abortion specimens.
Yi-Fang Dai, Xiao-Qing Wu, Hai-Long Huang, Shu-Qiong He, Dan-Hua Guo, Ying Li, Na Lin, Liang-Pu Xu   +7 more
doaj   +1 more source