Results 81 to 90 of about 15,621,385 (284)

Evaluation of a High-Throughput Dense Single-Nucleotide Polymorphism PCR Multiplex Next Generation Sequencing Application for Human Remains Identification

Forensic Genomics, 2023
The need to identify large numbers of missing people from mass disasters, armed conflicts, and human rights abuses has become increasingly prevalent. DNA methods for disaster victim identification (DVI) typically rely on the identification of postmortem (PM) samples through comparison to antemortem (AM) samples from close relatives
Sarah M. Radecke, Joana Antunes, June Snedecor, Clare Zlatkov, Laurence Devesse, Gothami Padmabandu, Kathryn M. Stephens   +6 more
openaire   +1 more source

Augmenting and Assaying Nav1.1 Protein Quantity for Dravet Syndrome Therapy

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Dravet Syndrome (DS) is a developmental and epileptic encephalopathy predominantly caused by heterozygous loss‐of‐function variants in SCN1A, which encodes Nav1.1. Conserved upstream open reading frames (uORFs) in SCN1A were validated to regulate translation in reporter assays, demonstrating the therapeutic viability of increasing Nav1.1 from ...
Aiswarya Saravanan, Jordan Safran, Anna Loughman, Grace Lin, Shivanshi Vaid, Dana Allababidi, Peter M. Tessier, Louis T. Dang   +7 more
wiley   +1 more source

An Overview of the Second and Third-Generation of DNA Sequencing Technologies [PDF]

Journal of Advanced Biomedical Sciences, 2017
DNA sequence determination is a tremendous human achievement. DNA sequencing includes several methods and technologies in use for determining the order of the nucleotide bases in a molecule of nucleic acid.
Fatemeh Ghorbani Parsa, Mohammad Ali Hossein Pour Feizi   +1 more
doaj  

LinkedSV for detection of mosaic structural variants from linked-read exome and genome sequencing data

Nature Communications, 2019
Compared to single nucleotide variants and short indels, structural variants (SVs) are often more challenging to detect using high-throughput sequencing based methods. Here, the authors develop LinkedSV, a computational tool for SV detection using linked-
Li Fang, Charlly Kao, Michael V. Gonzalez, Fernanda A. Mafra, Renata Pellegrino da Silva, Mingyao Li, Sören-Sebastian Wenzel, Katharina Wimmer, Hakon Hakonarson, Kai Wang   +9 more
doaj   +1 more source

A Complete Mitochondrial Genome Sequence from a Mesolithic Wild Aurochs (Bos primigenius) [PDF]

, 2010
Background The derivation of domestic cattle from the extinct wild aurochs (Bos primigenius) has been well-documented by archaeological and genetic studies.
Finlay Emma K., Park Stephen D. E., Daniel G Bradley, Shapiro Beth, Edwards Ceiridwen J., Ceiridwen J Edwards, Murphy, A., MacHugh David E., Beth Shapiro, Edwards, C.J., Bradley Daniel G., Park, S.D.E., MacHugh, D.E., McGettigan, Paul A., Shapiro, Beth, Alison Murphy, Martin B Richards, Chamberlain Andrew T., Loftus Brendan J., Murphy, Alison, Bradley, D.G., Knapp, Michael, David A Magee, Amanda J Lohan, Magee David A., Stephen D E Park, Lohan, A.J., Shapiro, B., Magee, David A., Bradley, Daniel G., Brendan J Loftus, Richards Martin B., Richards, M.B., Paul A McGettigan, Loftus, Brendan J., Park, Stephen D E, David E MacHugh, Chamberlain, Andrew T., Murphy Alison, MacHugh, David E., Andrew T Chamberlain, Park, Stephen D. E., Lohan, Amanda J., Lohan Amanda J., Loftus, B.J., Finlay, Emma K., McGettigan, P.A., Finlay, E.K., Richards, Martin B., Magee, D.A., Emma K Finlay, McGettigan Paul A., Chamberlain, A.T., Edwards, Ceiridwen J.   +53 more
core   +1 more source

Optical Detection of Cellular Signals at Material Interfaces

Advanced Healthcare Materials, EarlyView.
Emerging functional materials are transforming optical detection of cellular signals. This review highlights optical techniques that exploit the unique optical properties of diverse materials to detect and quantify cellular electrical, chemical, and mechanical signals, and discusses key opportunities and challenges.
Xuchen Ren, Jitong Ren, Zixin Zhang, Koney Eungkon Kim, Francis L. Hong, Yuecheng Peter Zhou   +5 more
wiley   +1 more source

High-throughput single nucleotide polymorphism (SNP) identification and mapping in the sesame (Sesamum indicum L.) genome with genotyping by sequencing (GBS) analysis [PDF]

Molecular Breeding, 2016
Sesame (Sesamum indicum L. syn. Sesamum orientale L.) is considered to be the first oil seed crop known to man. Despite its versatile use as an oil seed and a leafy vegetable, sesame is a neglected crop and has not been a subject of molecular genetic research until the last decade.
Uncu, Ayse Ozgur, Frary, Anne, Karlovsky, Petr, Doganlar, Sami   +3 more
openaire   +4 more sources

Genotyping by PCR and High-Throughput Sequencing of Commercial Probiotic Products Reveals Composition Biases.

Frontiers in Microbiology, 2016
Recent advances in microbiome research have brought renewed focus on beneficial bacteria, many of which are available in food and dietary supplements. Although probiotics have historically been defined as microorganisms that convey health benefits when ...
Wesley Morovic, Ashley A. Hibberd, Bryan Zabel, Rodolphe Barrangou, Buffy Stahl   +4 more
doaj   +1 more source

A Hybrid Bioprinting–Microfabrication Approach to Controllable Multitype Spheroid Arrays for Modeling Tumor Heterogeneity

Advanced Healthcare Materials, EarlyView.
This work introduces a “cancer‐in‐a‐well” technology that recreates tumor heterogeneity in vitro using multitype spheroid arrays. Combining bioprinting/microdispensing with microstructured culture devices, the system improves drug screening and modeling of tumor complexity.
Simone Bonetti, Eva Clemente, Emanuela Mensà, Marco de Maria, Fulvia Farabegoli, Emanuela Saracino, Barbara Bortolani, Emanuela Marcelli, Renato Brandimarti, Dario de Biase, Giampaolo Zuccheri   +10 more
wiley   +1 more source

Species-level core oral bacteriome identified by 16S rRNA pyrosequencing in a healthy young Arab population

Journal of Oral Microbiology, 2016
Background: Reports on the composition of oral bacteriome in Arabs are lacking. In addition, the majority of previous studies on other ethnic groups have been limited by low-resolution taxonomic assignment of next-generation sequencing reads. Furthermore,
Nezar Noor Al-hebshi, Ahmed Abdulhaq, Ahmed Albarrag, Vinod Kumar Basode, Tsute chen   +4 more
doaj   +1 more source