Results 71 to 80 of about 15,621,385 (284)

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

High, clustered, nucleotide diversity in the genome of Anopheles gambiae revealed through pooled-template sequencing: implications for high-throughput genotyping protocols [PDF]

BMC Genomics, 2009
Association mapping approaches are dependent upon discovery and validation of single nucleotide polymorphisms (SNPs). To further association studies in Anopheles gambiae we conducted a major resequencing programme, primarily targeting regions within or close to candidate genes for insecticide resistance.Using two pools of mosquito template DNA we ...
Wilding, CS, Weetman, D, Steen, K, Donnelly, MJ   +3 more
openaire   +4 more sources

Experience of copy number variation sequencing applied in spontaneous abortion

BMC Medical Genomics
Purpose We evaluated the value of copy number variation sequencing (CNV-seq) and quantitative fluorescence (QF)-PCR for analyzing chromosomal abnormalities (CA) in spontaneous abortion specimens.
Yi-Fang Dai, Xiao-Qing Wu, Hai-Long Huang, Shu-Qiong He, Dan-Hua Guo, Ying Li, Na Lin, Liang-Pu Xu   +7 more
doaj   +1 more source

Uninformative polymorphisms bias genome scans for signatures of selection [PDF]

, 2012
With the establishment of high-throughput sequencing technologies and new methods for rapid and extensive single nucleotide (SNP) discovery, marker-based genome scans in search of signatures of divergent selection between populations occupying ...
Daniel Berner, Roesti Marius, Berner Daniel, Marius Roesti, Salzburger Walter, Walter Salzburger, Salzburger, Walter, Berner, Daniel, Roesti, Marius   +8 more
core   +1 more source

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Additional file 3 of Correcting nucleotide-specific biases in high-throughput sequencing data

, 2017
Figure S2. ChIP-seq and DNase-seq coverage in a super enhancer region (Hnisz D, Abraham BJ, Lee TI, et al. Transcriptional super-enhancers connected to cell identity and disease. Cell. 2013;155(4):10.1016/j.cell.2013.09.053). This region is also in a DNase hypersensitivity region.
Wang, Jeremy, Quach, Bryan, Furey, Terrence   +2 more
openaire   +1 more source

Single nucleotide variant detection in Jaffrabadi buffalo (Bubalus bubalis) using high-throughput targeted sequencing [PDF]

Frontiers in Life Science, 2015
The water buffalo is among the most important livestock species of southern Asia, contributing greatly to the ecosystem and rural livelihood of the region. The identification of large-scale single nucleotide polymorphisms in this species would greatly facilitate our understanding of the genetic basis of economically important traits such as milk ...
R. Upadhyay, Maulik, B. Patel, Anand, B. Subramanian, Ramalingam, M. Shah, Tejas, J. Jakhesara, Subhash, D. Bhatt, Vaibhav, G. Koringa, Prakash, N. Rank, Dharamshibhai, G. Joshi, Chaitanya   +8 more
openaire   +1 more source

A pipeline for high throughput detection and mapping of SNPs from EST databases [PDF]

, 2010
Single nucleotide polymorphisms (SNPs) represent the most abundant type of genetic variation that can be used as molecular markers. The SNPs that are hidden in sequence databases can be unlocked using bioinformatic tools.
C. Gerard van der Linden, Anithakumari, A.M., Jack A. M. Leunissen, Richard G. F. Visser, Eck, H.J., van, A. M. Anithakumari, van Eck, H.J., Vosman, B., Visser, R.G.F., Tang, Jifeng, Herman J. van Eck, Leunissen, J.A.M., van der Linden, C.G., Linden, C.G., van der, Jifeng Tang, Ben Vosman   +15 more
core   +1 more source

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

FEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta, Sebastian GB Furness, Tahereh Gharbi, Ric De Paoli‐Iseppi, Shweta S. Joshi, Michael Clark, David L. Hare, Peter Wookey   +7 more
wiley   +1 more source

Additional file 2 of Correcting nucleotide-specific biases in high-throughput sequencing data

, 2017
Table S1. Area under curve (AUC) values for the ROC curves representing sensitivity and specificity of footprint detection for several transcription factors. AUC values at increasing false positive rates (FPR) are computed independently for each motif before and after correction.
Wang, Jeremy, Quach, Bryan, Furey, Terrence   +2 more
openaire   +1 more source