Results 51 to 60 of about 15,621,385 (284)

High‐throughput capture of nucleotide sequence polymorphisms in three Brassica species (Brassicaceae) using DNA microarrays [PDF]

open access: yesAmerican Journal of Botany, 2012
• Premise of the study: To capture molecular markers that are applicable to environmental risk assessment of genetically modified oilseed rape, and to streamline their development, we screened variations in nucleotide sequences of three Brassica species by DNA microarray analysis.• Methods and Results: Using the Affymetrix GeneChip Arabidopsis ATH1 ...
Toru, Nishizawa   +6 more
openaire   +2 more sources

Design of a high density SNP genotyping assay in the pig using SNPs identified and characterized by next generation sequencing technology [PDF]

open access: yes, 2009
A systematic review of the literature was carried out to determine the evidence for the reliability and validity of the assessment methods used in veterinary medical education. The review followed the approach used by the Best Evidence Medical Education (
Rothschild, M. F.   +133 more
core   +1 more source

Structural variation in the chicken genome identified by paired-end next-generation DNA sequencing of reduced representation libraries [PDF]

open access: yes, 2011
Background Variation within individual genomes ranges from single nucleotide polymorphisms (SNPs) to kilobase, and even megabase, sized structural variants (SVs), such as deletions, insertions, inversions, and more complex rearrangements.
Kerstens Hindrik HD   +17 more
core   +2 more sources

High-throughput SuperSAGE for digital gene expression analysis of multiple samples using next generation sequencing [PDF]

open access: yes, 2010
We established a protocol of the SuperSAGE technology combined with next-generation sequencing, coined "High- Throughput (HT-) SuperSAGE". SuperSAGE is a method of digital gene expression profiling that allows isolation of 26-bp tag fragments from ...
Schroth, G.P.   +68 more
core   +1 more source

Next-Generation Sequencing Reveals Differentially Expressed Genes and Pathways in Urethral Cancer: Exploring A Poorly Understood Malignancy [PDF]

open access: yesJournal of Urologic Oncology
Purpose Primary urethral cancer (PUC) is an uncommon malignancy with scarce diagnostic and treatment options, resulting in a limited understanding of its genetic foundation. This exploratory study compares gene expression profiles between urethral cancer
Nickolas Kinachtchouk   +7 more
doaj   +1 more source

Construction and analysis of tag single nucleotide polymorphism maps for six human-mouse orthologous candidate genes in type I diabetes. [PDF]

open access: yes, 2005
BACKGROUND: One strategy to help identify susceptibility genes for complex, multifactorial diseases is to map disease loci in a representative animal model of the disorder. The nonobese diabetic (NOD) mouse is a model for human type 1 diabetes.
Undlien, Dag E   +74 more
core   +11 more sources

Diversity and complexity in neural organoids

open access: yesFEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

Chromosomal analyses of human giant diploid oocytes by next‐generation sequencing

open access: yesReproductive Medicine and Biology, 2021
Purpose Although giant oocytes (GOs) having about twice cytoplasmic volume compared with general oocytes in mammals including the human are rarely recovered, it is thought that GOs have potentially chromosomal abnormalities.
Hiroomi Kawano   +3 more
doaj   +1 more source

Biochemical and structural characterization of mycobacterial aspartyl-tRNA synthetase AspS, a promising TB drug target [PDF]

open access: yes, 2014
The human pathogen Mycobacterium tuberculosis is the causative agent of pulmonary tuberculosis (TB), a disease with high worldwide mortality rates. Current treatment programs are under significant threat from multi-drug and extensively-drug resistant ...
VijayaShankar Nataraj   +75 more
core   +1 more source

An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes

open access: yesFEBS Letters, EarlyView.
GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg   +14 more
wiley   +1 more source

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