Results 41 to 50 of about 15,621,385 (284)

PIMS sequencing extension:a laboratory information management system for DNA sequencing facilities [PDF]

open access: yes, 2011
Background: Facilities that provide a service for DNA sequencing typically support large numbers of users and experiment types. The cost of services is often reduced by the use of liquid handling robots but the efficiency of such facilities is hampered ...
McPherson, MJ   +18 more
core   +1 more source

DNA-based faecal dietary analysis: A comparison of qPCR and high throughput sequencing approaches [PDF]

open access: yes, 2011
The genetic analysis of faecal material represents a relatively non-invasive way to study animal diet and has been widely adopted in ecological research.
Michael Bunce   +38 more
core   +1 more source

High-throughput nucleotide sequence analysis of diverse bacterial communities in leachates of decomposing pig carcasses

open access: yesGenetics and Molecular Biology, 2015
The leachate generated by the decomposition of animal carcass has been implicated as an environmental contaminant surrounding the burial site. High-throughput nucleotide sequencing was conducted to investigate the bacterial communities in leachates from the decomposition of pig carcasses. We acquired 51,230 reads from six different samples (1, 2, 3, 4,
Yang, Seung Hak   +6 more
openaire   +6 more sources

DUDE-Seq: Fast, flexible, and robust denoising for targeted amplicon sequencing. [PDF]

open access: yesPLoS ONE, 2017
We consider the correction of errors from nucleotide sequences produced by next-generation targeted amplicon sequencing. The next-generation sequencing (NGS) platforms can provide a great deal of sequencing data thanks to their high throughput, but the ...
Byunghan Lee   +3 more
doaj   +1 more source

A bioinformatics approach to microRNA-sequencing analysis

open access: yesOsteoarthritis and Cartilage Open, 2021
The rapid expansion of Next Generation Sequencing (NGS) data availability has made exploration of appropriate bioinformatics analysis pipelines a timely issue.
Pratibha Potla   +2 more
doaj   +1 more source

Multi-Platform Next-Generation Sequencing of the Domestic Turkey (Meleagris gallopavo): Genome Assembly and Analysis [PDF]

open access: yes, 2010
A synergistic combination of two next-generation sequencing platforms with a detailed comparative BAC physical contig map provided a cost-effective assembly of the genome sequence of the domestic turkey (Meleagris gallopavo).
Kent M Reed   +405 more
core   +1 more source

High-throughput amplicon sequencing of the full-length 16S rRNA gene with single-nucleotide resolution

open access: yesNucleic Acids Research, 2018
Abstract Targeted PCR amplification and high-throughput sequencing (amplicon sequencing) of 16S rRNA gene fragments is widely used to profile microbial communities. New long-read sequencing technologies can sequence the entire 16S rRNA gene, but higher error rates have limited their attractiveness when accuracy is important.
Callahan, Benjamin J   +7 more
openaire   +2 more sources

Genetic, Clinicopathological, and Radiological Features of Intrahepatic Cholangiocarcinoma with Ductal Plate Malformation Pattern

open access: yesGut and Liver, 2022
Background/Aims: Intrahepatic cholangiocarcinoma (iCCA) with a ductal plate malformation (DPM) pattern is a recently recognized rare variant. The genomic profile of iCCA with DPM pattern needs to be elucidated.
Taek Chung   +6 more
doaj   +1 more source

Special features of RAD Sequencing data:implications for genotyping [PDF]

open access: yes, 2012
Restriction site-associated DNA Sequencing (RAD-Seq) is an economical and efficient method for SNP discovery and genotyping. As with other sequencing-by-synthesis methods, RAD-Seq produces stochastic count data and requires sensitive analysis to develop ...
Fuentes-Utrilla, Pablo   +12 more
core   +1 more source

Quantifying single nucleotide variant detection sensitivity in exome sequencing [PDF]

open access: yes, 2013
BACKGROUND: The targeted capture and sequencing of genomic regions has rapidlydemonstrated its utility in genetic studies. Inherent in this technology isconsiderable heterogeneity of target coverage and this is expected tosystematically impact our ...
Taylor, Martin S.; id_orcid   +8 more
core   +1 more source

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