Results 71 to 80 of about 571,215 (288)

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Structure and evolution of protoplanetary disks [PDF]

, 2008
We present here a few thoughts on how high-angular resolution observations can give clues to some properties of protoplanetary disks that are fundamental to theories of planet formation.
Apai, Daniel, Dullemond, Cornelis, Pavlyuchenkov, Yaroslav, Pontoppidan, Klaus   +3 more
core   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Imaging exoplanets with coronagraphic instruments

Comptes Rendus. Physique, 2023
Exoplanetary science is a very active field of astronomy nowadays, with questions still opened such as how planetary systems form and evolve (occurrence, process), why such a diversity of exoplanets is observed (mass, radius, orbital parameters ...
Galicher, Raphaël, Mazoyer, Johan
doaj   +1 more source

High angular resolution measurements of Algol

The Astrophysical Journal, 1993
Algol (Beta Per) is an extensively studied triple system which includes an eclipsing pair. Using long baseline optical interferometry, the AB-C system of Algol has had its geometric and physical parameters determined with higher resolution and accuracy than obtained with other techniques.
Xiaopei Pan, Michael Shao, M. M. Colavita   +2 more
openaire   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Stellar Populations in the Central 0.5 pc of Our Galaxy. III. The Dynamical Substructures

The Astrophysical Journal, 2023
We measure the 3D kinematic structures of the young stars within the central 0.5 pc of our Galactic Center using the 10 m telescopes of the W. M. Keck Observatory over a time span of 25 yr.
Siyao Jia, Ningyuan Xu, Jessica R. Lu, D. S. Chu, K. Kosmo O’Neil, W. B. Drechsler, M. W. Hosek Jr., S. Sakai, T. Do, A. Ciurlo, A. K. Gautam, A. M. Ghez, E. Becklin, M. R. Morris, R. O. Bentley   +14 more
doaj   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Time Evolution Images of the Hypergiant RW Cephei during the Rebrightening Phase Following the Great Dimming

The Astrophysical Journal Letters
Stars with initial masses larger than 8 M _⊙ undergo substantial mass loss through mechanisms that remain elusive. Unraveling the origins of this mass loss is important for comprehending the evolutionary path of these stars, the type of supernova ...
Narsireddy Anugu, Douglas R. Gies, Rachael M. Roettenbacher, John D. Monnier, Miguel Montargés, Antoine Mérand, Fabien Baron, Gail H. Schaefer, Katherine A. Shepard, Stefan Kraus, Matthew D. Anderson, Isabelle Codron, Tyler Gardner, Mayra Gutierrez, Rainer Köhler, Karolina Kubiak, Cyprien Lanthermann, Olli Majoinen, Nicholas J. Scott, Wolfgang Vollmann   +19 more
doaj   +1 more source

Southern Massive Stars at High Angular Resolution: Observational Campaign and Companion Detection [PDF]

, 2014
Multiplicity is one of the most fundamental observable properties of massive O-type stars and offers a promising way to discriminate between massive star formation theories.
Absil, O., Berger, J. -P., Bouquin, J. -B. Le, de Koter, A., Duvert, G., Gauchet, L., Kratter, K., Lacour, S., Norris, B., Olofsson, J., Pickel, D., Sana, H., Schnurr, O., Zinnecker, H., Zins, G.   +14 more
core   +5 more sources