Results 211 to 220 of about 21,693,337 (313)

Versatile vector tools for efficient protein screening across multiple expression systems

open access: yesFEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu   +5 more
wiley   +1 more source

Broadband backscattering confocal microscopy enables label-free 3D live cell nanoscale sensitive imaging. [PDF]

open access: yesSci Adv
Coughlan MF   +8 more
europepmc   +1 more source

Development of bolometric tomography technique for high-contrast radiation distribution in toroidal devices

open access: yesDevelopment of bolometric tomography technique for high-contrast radiation distribution in toroidal devices
An extended Computed Tomography (CT) technique with a priori information based on Phillips-Tikhonov regularization has been developed to handle a high-contrast radiation distribution, which can result in large reconstruction errors in the region where radiation intensity is low, with few line of sight (LOS) data.
openaire   +1 more source

High contrast imaging techniques using light coherence for exoplanet direct detection.

open access: yes, 2009
Only 3% of known exoplanets have been directly detected up to now for several reasons. First, they are very close to their parent star, which sets a minimum telescope size and requires the use of an atmospheric turbulence compensator. Then, the star is so bright compared to its planets (one million to ten billions) that the reduction of its light ...
openaire   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

open access: yesFEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová   +2 more
wiley   +1 more source

Magnetic resonance imaging. [PDF]

open access: yesSci Adv
Blümich B   +6 more
europepmc   +1 more source

Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids

open access: yesFEBS Open Bio, EarlyView.
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão   +3 more
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

open access: yesFEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova   +2 more
wiley   +1 more source

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