Emerging experimental and computational methods for studying redox‐regulated structural transitions
Redox reactions can reshape proteins and alter how they behave in cells, with important consequences for health and disease. This review explores emerging experimental and computational approaches for discovering these redox‐sensitive protein switches, revealing their structural effects, and predicting their behavior, opening new opportunities to ...
Tasneem Rass +2 more
wiley +1 more source
Interfacing complex laboratory instruments during a change to epic beaker
Background: Implementing a laboratory-developed test sometimes requires incorporating an unconventional device into the laboratory information system (LIS) and customizing an interface to reduce transcription error and improve turnaround time.
Gregory David Scott +5 more
doaj +1 more source
Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança +2 more
wiley +1 more source
The castings defects are affected by the melting volume change rate of material. The change rate has an important effect on running safety of the high temperature thermal storage chamber, too. But the characteristics of existing measuring installations are complex structure, troublesome operation and low precision.
Junwei Wang +3 more
openaire +1 more source
Elimination of Instrument-Driven Reflex Manual Differential Leukocyte Counts [PDF]
Automated peripheral blood leukocyte differential counts (LDCs) are widely accepted in routine practice. However, many laboratories still reflexively perform manual LDCs based solely on abnormal automated results or instrument "flags," before any manual triage step.
Kay L, Lantis +4 more
openaire +2 more sources
Structural and biochemical analysis of a B12 superbinder
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices +3 more
wiley +1 more source
CO measurements from the ACE-FTS satellite instrument: data analysis and validation using ground-based, airborne and spaceborne observations [PDF]
The Atmospheric Chemistry Experiment (ACE) mission was launched in August 2003 to sound the atmosphere by solar occultation. Carbon monoxide (CO), a good tracer of pollution plumes and atmospheric dynamics, is one of the key species provided by the ...
Emmons, Louisa +185 more
core +2 more sources
The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge +6 more
wiley +1 more source
In-flight Monitoring of TerraSAR-X Radar Instrument Stability [PDF]
For calibrating and monitoring the radiometric stability, the radar instrument of TerraSAR-X hosts a passive calibration network coupling into an additional port of the T/R modules. Three different types of calibration pulses are applied, whereby sets of
Schwerdt, Marco +2 more
core
Implementation of Vibrancy Awaking Pressure Measuring Instrument for Single Hydraulic Prop
An instrument awakened by means of vibration for single hydraulic prop pressure measuring is described in this paper.The principle and implementation of this method are introduced in detail.The instrument uses the high-performance single chip C8051F310 ...
Feng-long ZHENG +4 more
doaj

