Results 231 to 240 of about 269,645 (307)

Purification, Fluorescent Labeling, and Detyrosination of Mammalian Cell Tubulin for Biochemical Assays

open access: yesCytoskeleton, EarlyView.
ABSTRACT Microtubules play essential roles in numerous cellular processes. All microtubules are built from the protein tubulin, yet individual microtubules can differ spatially and temporally due to their tubulin isotype composition and post‐translational modifications (PTMs).
Ezekiel C. Thomas   +5 more
wiley   +1 more source

SABRE Ir-IMes Catalysis for the Masses. [PDF]

open access: yesMolecules
Smith I   +13 more
europepmc   +1 more source

Expression, Purification, and Microscopy‐Based Assays for Engineered Recombinant Tyrosinated, Detyrosinated, and Δ2 Human Tubulin

open access: yesCytoskeleton, EarlyView.
ABSTRACT Microtubules are noncovalent polymers assembled from α/β tubulin dimers. Their structure, dynamics and interaction with effectors are regulated through the expression of diverse tubulin isotypes and chemically diverse posttranslational modifications, also known as the “tubulin code.” Understanding the biophysical correlates between tubulin ...
Jiayi Chen   +2 more
wiley   +1 more source

A Facile and Reproducible Method for the Purification of Peptide- and Protein-Functionalized DNA Nanostructures. [PDF]

open access: yesJACS Au
Schärf I   +9 more
europepmc   +1 more source

A Facile Protocol for C(sp2)–C(sp3) Bond Formation Reactions Toward Functionalized E3 Ligase Ligands

open access: yesChemMedChem, EarlyView.
A robust C(sp2)–C(sp3) decarboxylative coupling strategy enables access to new CRBN ligands and degraders with improved physicochemical properties. This synthetic approach is expanding the chemical space beyond C(sp2)–N linkages, fine‐tuning proteolysis‐targeting chimera activities and unlocking previously inaccessible degrader chemotypes.
Anita Maksutova   +15 more
wiley   +1 more source

An Ultra‐Low‐Power and High‐Temperature‐Homogeneity Wafer‐Scale Infrared Source

open access: yesENERGY &ENVIRONMENTAL MATERIALS, EarlyView.
A wafer‐scale MEMS infrared source with an Al@nanoforest radiation layer achieves high emissivity and temperature homogeneity at low power, enabling efficient CO2 detection for smart agriculture. Non‐dispersive infrared gas sensors, renowned for their high selectivity and high reliability, are extensively employed in applications of smart agriculture ...
Qirui Zhang   +9 more
wiley   +1 more source

Titan's strong tidal dissipation precludes a subsurface ocean. [PDF]

open access: yesNature
Petricca F   +15 more
europepmc   +1 more source

Energy Storage System in Microgrids: Challenges and Opportunities

open access: yesEnergy Science &Engineering, EarlyView.
Hybrid integration of multiple Energy Storage Systems (ESSs) within renewable‐powered microgrids enhances reliability, flexibility, and economic sustainability. Lithium‐ion batteries, flow batteries, hydrogen storage, and thermal systems complement each other through coordinated control via Energy Management Systems.
Mohamed G. Moh Almihat, Josiah L. Munda
wiley   +1 more source

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