Results 131 to 140 of about 156,550 (306)

Nonviral Gold Nanoparticle‐Mediated Delivery of CRISPR‐Cas9 Ribonucleoprotein and Long DNA Transgenes Into Primary Blood Cells

Advanced NanoBiomed Research, EarlyView.
CRISPR/Cas9 has revolutionized the field of gene therapy, but delivery remains an outstanding issue. We propose a nonviral gold‐nanoparticle platform for co‐delivery of CRISPR/Cas9 ribonucleoprotein and long 2.1 kilobase dsDNA transgene constructs. This CRISPR‐AuNP is inexpensive to produce and mediate gene editing and DNA delivery in T cells and CD34+
Rachel A. Cunningham, Karthikeya S. V. Gottimukkala, Daniel D. Lane, Katrina Poljakov, Patricia Lipson, Mark R. Enstrom, Alessandro Rizzi, Aude G. Chapuis, Jennifer E. Adair   +8 more
wiley   +1 more source

Combinatorial latency reversal activity of inhibitor of apoptosis antagonists with mechanistically distinct classes of HIV latency reversal agents

Journal of Virus Eradication, 2019
Shane D. Falcinelli, David M. Irlbeck, Anne-Marie Turner, Frances Potjewyd, Lindsey I. James, David M. Margolis, Nancie M. Archin, Richard M. Dunham   +7 more
doaj   +1 more source

Chicken Pulmonary MicroRNAs Targeting the PB2 (Segment 1) of Avian Influenza Virus

Animal Research and One Health, EarlyView.
The PB2 segment of H5N1 is essential for replication and host adaptation. We screened 200 miRNAs and identified five (gga‐miR‐17‐3p, gga‐miR‐29a‐5p, gga‐miR‐1718, gga‐miR‐16c‐5p, and gga‐miR‐1744‐5p) using thermodynamic stability of heteroduplex, seed sequence complementarity, conservation, and accessibility, offering insights into host antiviral ...
Akanksha Choudhary, Nidhishree N. S, Rakesh Kumar Pundir, Muhasin Asaf V. N, Anamika Mishra, Ashwin Ashok Raut, Megha Agarwal, Amod Kumar   +7 more
wiley   +1 more source

OP 1.4 – 00053 Definitive evidence of a persistent HIV reservoir in human brain myeloid cells despite ART

Journal of Virus Eradication, 2022
Y. Tang, G. Jiang, M. Porrachia, C. Ignacio, L.M. Wong, D. Zhong, J. Du, B. Cotsakis, S. Maske, E. de la Parra Polina, T.L. Simermeyer, D. Li, B. Woodworth, J. Kirchherr, B. Allard, M. Clohosey, G.D. Whitehill, J.J. Eron, N.M. Archin, S.B. Joseph, K. Bar, A. Chaillon, S. Gianella, D.M. Margolis, G. Jiang   +24 more
doaj   +1 more source

Development and Evaluation of a Novel Relatively Low-Cost Method to Derive HIV-1 Integration Sites and Proviral Sequences

Viruses
In people taking antiretroviral therapy (ART) for HIV infection, the methods to characterize latent and active HIV reservoirs remain costly and labor-intensive.
Samantha R. Hardy, Sheila Styrchak, Tim De Meyer, Laurens Lambrechts, Tine Struyve, Basiel Cole, Liesbet Termote, Sherry McLaughlin, James I. Mullins, Linos Vandekerckhove, Lisa M. Frenkel   +10 more
doaj   +1 more source

HIV “cure”: A shot in the arm? [PDF]

EBioMedicine, 2019
Peluso, Michael J., Deeks, Steven G., McCune, Joseph M.   +2 more
openaire   +2 more sources

Long‐Term Outcomes of Reduced‐Intensity Conditioning Hematopoietic Stem Cell Transplantation for Patients With Systemic Sclerosis With Impaired Cardiac Function

Arthritis &Rheumatology, EarlyView.
Objective High‐intensity conditioning autologous hematopoietic stem cell transplantation (AHSCT) is standard of care for patients with advanced systemic sclerosis (SSc). The role of reduced‐intensity conditioning (RIC) before AHSCT in this population remains unclear.
Yonatan Lean, Carrie Richardson, Anthony J. Esposito, Katy Bedjeti, George E. Georges   +4 more
wiley   +1 more source

Exploring sequence specific silencing of latent HIV using CRISPR interference

Journal of Virus Eradication, 2019
R. Schwarzer, M. Montano, W.C. Greene
doaj   +1 more source

Retinol Binding Protein 4 reactivates latent HIV-1 by triggering canonical NF-κB, JAK/STAT5 and JNK signalling

Signal Transduction and Targeted Therapy
Reactivation of the latent viral reservoirs is crucial for a cure of HIV/AIDS. However, current latency reversing agents are inefficient, and the endogenous factors that have the potential to reactivate HIV in vivo remain poorly understood.
Chiara Pastorio, Khumoekae Richard, Shariq Usmani, Ann-Kathrin Kissmann, Grigory Bolotnikov, Guillermo Gosálbez, Manuel Hayn, Lennart Koepke, Alina Sauertnik, Andrea Preising, Nico Preising, Ludger Ständker, Matthew Fair, Jessicamarie Morris, Emmanouil Papasavvas, Qin Liu, Honghong Sun, Armando Rodríguez, Karam Mounzer, Sebastian Wiese, Pablo Tebas, Yangzhu Du, Gregory M. Laird, Markus Jaritz, Frank Rosenau, Moritz M. Gaidt, Konstantin M. J. Sparrer, Luis J. Montaner, Frank Kirchhoff   +28 more
doaj   +1 more source

Machine Learning to Predict Remission Between 6 and 24 Months in Rheumatoid Arthritis: Insights From JAK, an International Registry Collaboration

Arthritis &Rheumatology, EarlyView.
Objective To develop, externally validate, and simplify a machine learning model to predict remission between 6 and 24 months in patients with rheumatoid arthritis (RA) initiating tumor necrosis factor inhibitors, JAK inhibitors, interleukin‐6 inhibitors, abatacept, or rituximab using data from 11 international registries in the JAK‐pot collaboration ...
Zubeyir Salis, Denis Mongin, Denis Choquette, Louis Coupal, Catalin Codreanu, Florenzo Iannone, Roberto Caporali, Tore K. Kvien, Sella Provan, Ruth Fritsch‐Stork, Dan Nordström, Nina Trokovic, Karel Pavelka, Jakub Závada, Ana Rodrigues, Ziga Rotar, Prodromos Sidiropoulos, Irini Flouri, Céline Lamacchia, Michele Iudici, Delphine Courvoisier, Kim Lauper, Axel Finckh   +22 more
wiley   +1 more source