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Homologous overexpression of genes in Cordyceps militaris improves the production of polysaccharides

Food Research International, 2021
The maximum yield of EPS produced by mutant CM-pgm-H was 4.63 ± 0.23 g/L, whereas the yield of wild-type strain was 3.43 ± 0.26 g/L. In addition, the data obtained in the present study indicated that the yield of EPS produced by the engineered strain treated with the co-overexpression of phosphoglucomutase and UDP-glucose 6-dehydrogenase genes achieved
Yifeng, Wang   +4 more
openaire   +2 more sources

Characterization and Homologous Overexpression of an N-Acetylglucosaminidase Nag1 From Trichoderma reesei

Biochemical and Biophysical Research Communications, 2015
Trichoderma reesei is thought to be a promising recombinant host for the production and secretion of complex proteins due to its ability to secrete large amounts of proteins. In this study we identified a functional N-acetyl-β-glucosaminidase (NAGase) gene Nag1 in T. reesei. Nag1, a putative gene encoding a GH 20 family NAGase in T.
Yong Tao
exaly   +3 more sources

Molecular Characterization and Homologous Overexpression of [FeFe]-Hydrogenase in Clostridium tyrobutyricum JM1

International Journal of Hydrogen Energy, 2010
Abstract The H2-evoving [FeFe]-hydrogenase in Clostridium tyrobutyricum JM1 was isolated to elucidate molecular characterization and modular structure of the hydrogenase. Then, homologous overexpression of the hydrogenase gene was for the first time performed to enhance hydrogen production.
Jong Moon Park   +2 more
exaly   +3 more sources

Homologous overexpression of a light‐driven anion pump in an archaebacterium

Molecular Microbiology, 1993
SummaryThe retinal protein halorhodopsin (HR), a light‐driven chloride pump from Halobacterium halobium, was homologously overexpressed in this archaebacterium. Two DNA expression systems differing in their promoter region were investigated. The halo‐opsin, hop, promoter coupled to the hop gene gave an increased level of HR synthesis.
J A, Heymann   +2 more
openaire   +2 more sources

Identification and characterization of FaSOC1, a homolog of SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 from strawberry

Gene, 2013
A MADS-box gene SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1) integrates multiple flowering signals to regulate floral transition in Arabidopsis. Strawberry (Fragaria spp.) is an economically important fruit crop, but its molecular control of flowering is largely unknown.
Heng-Jiu, Lei   +7 more
openaire   +2 more sources

Overexpression of OsRecQl4 and/or OsExo1 Enhances DSB-Induced Homologous Recombination in Rice

Plant and Cell Physiology, 2012
During homologous recombination (HR)-mediated DNA double-strand break (DSB) repair in eukaryotes, an initial step is the creation of a 3'-single-stranded DNA (ssDNA) overhang via resection of a 5' end. Rad51 polymerizes on this ssDNA to search for a homologous sequence, and the gapped sequence is then repaired using an undamaged homologous DNA strand ...
Yong-Ik, Kwon   +7 more
openaire   +2 more sources

Overexpression of Bacterial RecA Protein Stimulates Homologous Recombination in Somatic Mammalian Cells

Mutation Research DNA Repair, 2000
The pairing of homologous molecules and strand exchange is a key event in homologous recombination promoted by RecA protein in Escherichia coli. Structural homologs of RecA are widely distributed in eukaryotes including mouse and man. As has been shown, human HsRad51 protein is not only structural but also functional homolog of RecA.
Olga G Shcherbakova   +2 more
exaly   +3 more sources

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