Results 171 to 180 of about 345,396 (332)
Molecular Insights into Fungal Glycosylphosphatidylinositol Transamidase Complex
Advanced Science, EarlyView.The glycosylphosphatidylinositol (GPI) biosynthesis pathway is critical for antifungal drug development. This study reports three cryo‐electron microscopy structures of fungal GPI transamidase (GPIT), captured in two substrate‐bound states and a novel dimeric assembly.
Zhengkang Hua +12 more
wiley +1 more source
Positive genetic selection for gene disruption in mammalian cells by homologous recombination.
, 1989 John M. Sedivy, Phillip A. Sharp
openalex +1 more source
Reversion of Q beta RNA phage mutants by homologous RNA recombination [PDF]
, 1992 Kampan Palasingam, Patrick N. Shaklee
openalex +1 more source
Advanced Science, EarlyView.A genome‐scale model guided the systematic metabolic engineering of Saccharopolyspora spinosa, enabling a 5.5‐fold boost in the green bioinsecticide spinosad to 1.8 g L−1 and establishing an actinomycete framework for complex natural products overproduction.
Shuliu Wang +9 more
wiley +1 more source
The Role and Fate of DNA Ends for Homologous Recombination in Embryonic Stem Cells [PDF]
, 1992 Paul Hasty +2 more
openalex +1 more source
CRISPR‐DNA Polymerase Assisted Targeted Mutagenesis for Regulable Laboratory Evolution
Advanced Science, EarlyView.This study develops an in vivo targeted mutagenesis system based on CRISPR‐guided bacteriophage T5 or T7 DNA polymerases. This system can enable all possible types of substitution mutations within an expanded mutagenesis window in Escherichia coli and exhibit application potential in different microorganisms, thus accelerating the evolution of ...
Shuaili Chen +8 more
wiley +1 more source
Adenovirus homologous recombination does not require expression of the immediate-early E1a gene [PDF]
, 1991 Lisa H. Epstein, C.S. Young
openalex +1 more source
Advanced Science, EarlyView.Green‐synthesized (ECMP)2MnBr4 achieves near‐unity photoluminescence quantum yield (98.97%) and triple‐mode emission (photo/X‐ray/mechano), enabling wearable anti‐counterfeiting textiles, ultrasensitive X‐ray imaging (15.62 nGyair s−1), and demonstrating potential for optical stress sensing.
Xiao Wang +11 more
wiley +1 more source
RPS27L Enhances Myogenesis and Muscle Mass by Targeting IGF1 Through Liquid‐Liquid Phase Separation
Advanced Science, EarlyView.Muscle‐specific RPS27L knock‐in mice exhibit increased body weight, muscle mass, and enhanced muscle regenerative capacity. RPS27L drives myogenesis by promoting myoblast proliferation, while inhibiting myogenic differentiation. Mechanistically, RPS27L targets IGF1 protein by liquid‐liquid phase separation through its N‐terminal intrinsically ...
Xiaoqin Liu +17 more
wiley +1 more source
RNA G‐Quadruplex RIBOTAC‐Mediated Targeted Degradation of lncRNA TERRA
Advanced Science, EarlyView.This study introduces a selective RNA degradation strategy targeting long noncoding RNA (lncRNA) TERRA, upregulated in ALT (alternative lengthening of telomeres) cancer cells. Using ribonuclease‐targeting chimeras (RIBOTACs), RNase L is recruited to catalytically degrade TERRA, uncovering a novel approach to affect a disease associated lncRNA in ALT ...
Elias Khaskia +2 more
wiley +1 more source