Results 171 to 180 of about 783,358 (391)
Advanced Science, Volume 12, Issue 12, March 27, 2025.The non‐conventional oleaginous yeast Y. lipolytica has been genetically engineered to produce malonic acid via malonyl‐CoA pathway through introducing an efficient malonyl‐CoA hydrolase. Advanced metabolic engineering and optimization of fermentation conditions resulted in a malonic acid production yield of 19.1 g L−1 in shaking‐flasks.
Qun Yang +4 more
wiley +1 more source
hESCs‐derived Organoids Achieve Liver Zonation Features through LSEC Modulation
Advanced Science, EarlyView.This article addresses the absence of zonation features in current organoid models by co‐culturing human embryonic stem cell‐derived hepatocytes with liver sinusoidal endothelial cells exhibiting distinct zonal characteristics. It demonstrates that differential WNT2 signaling from endothelial cells modulates hepatocyte zonation and metabolic functions,
Yuying Zhang +13 more
wiley +1 more source
Progressive Mauve: Multiple alignment of genomes with gene flux and rearrangement [PDF]
arXiv, 2009 Multiple genome alignment remains a challenging problem. Effects of recombination including rearrangement, segmental duplication, gain, and loss can create a mosaic pattern of homology even among closely related organisms. We describe a method to align two or more genomes that have undergone large-scale recombination, particularly genomes that have ...
arxiv
Production of chimaeric mice containing embryonic stem (ES) cells carrying a homoeobox Hox 1.1 allele mutated by homologous recombination [PDF]
, 1989 Andreas Zimmer, Peter Gruss
openalex +1 more source
Advanced Science, Volume 12, Issue 14, April 10, 2025.An engineered Escherichia coli is developed to efficiently produce adipic acid (AA) from cyclohexanol, KA oil (a mixture of cyclohexanol and cyclohexanone), or cyclohexane through a combination of enzyme screening, protein fusion, and genome editing techniques. This approach has achieved the highest recorded productivity for biological AA production to
Fei Wang +6 more
wiley +1 more source
Optimizing Gateway™ technology (Invitrogen) to construct Rhizobium leguminosarum deletion mutants [PDF]
, 2010 The study of the role of different genes in Rhizobium leguminosarum requires the generation of mutants by homologous recombination. In this communication we describe a novel approach to obtain deletion mutants of genes in Rhizobium using Gateway TM ...
Alborno, Marcelo +3 more
core
Advanced Science, Volume 12, Issue 17, May 8, 2025.Ca2+/calmodulin‐dependent kinase II (CaMKII) activation is likely driven by oxidative stress, particularly excessive reactive oxygen species (ROS) production. This study identifies P21‐activated kinase 2 (Pak2) as a novel regulator of ROS‐induced CaMKII activation and abnormal Ca2⁺ dynamics in acute adrenergic and chronic pressure‐overload stressed ...
Tao Li +21 more
wiley +1 more source
The Impact of Homologous Recombination on Silent Chromatin in Saccharomyces cerevisiae [PDF]
, 2017 Specialized chromatin domains repress transcription of genes within them and present a barrier to many DNA-protein interactions. Silent chromatin in the budding yeast Saccharomyces cerevisiae, akin to heterochromatin of metazoans and plants, is imparted ...
Sieverman, Kathryn Jeanne
core
Homologous recombination of SV4O DNA in COS7 cells occurs with high frequency m a gene dose independent fashion [PDF]
, 1988 W Rommerskirch +3 more
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Advanced Science, Volume 12, Issue 16, April 24, 2025.KLF5 is highly expressed in BLBC patients and promotes Cyclin D1 and XPO1 transcription, ultimately leading to the inactivation of RB1. Both XPO1 inhibitor (KPT‐330) and CDK4/6 inhibitor (Palbociclib) can enhance RB1 activity and exert antitumor effects. It has been proved that the combination of KPT‐330 and Palbociclib can inhibit the proliferation of
Yu Tang +12 more
wiley +1 more source