Results 71 to 80 of about 233,309 (305)

Expression of EhRAD54, EhRAD51, and EhBLM proteins during DNA repair by homologous recombination in Entamoeba histolytica

Parasite, 2014
Entamoeba histolytica, the protozoan responsible for human amoebiasis, exhibits a great genome plasticity that is probably related to homologous recombination events.
del Socorro Charcas-Lopez Ma., Garcia-Morales Lorena, Pezet-Valdez Marisol, Lopez-Camarillo Cesar, Zamorano-Carrillo Absalom, Marchat Laurence A.   +5 more
doaj   +1 more source

Variation in RAD51 details a hub of functions: opportunities to advance cancer diagnosis and therapy [version 2; referees: 4 approved]

F1000Research, 2018
Loss of genome stability is one of the hallmarks of the enabling characteristics of cancer development. Homologous recombination is a DNA repair process that often breaks down as a prelude to developing cancer. Conversely, homologous recombination can be
Nick LL van der Zon, Roland Kanaar, Claire Wyman   +2 more
doaj   +1 more source

PCR artifact in testing for homologous recombination in genomic editing in zebrafish. [PDF]

PLoS ONE, 2017
We report a PCR-induced artifact in testing for homologous recombination in zebrafish. We attempted to replace the lnx2a gene with a donor cassette, mediated by a TALEN induced double stranded cut.
Minho Won, Igor B Dawid
doaj   +1 more source

Finding novel vulnerabilities of hypomorphic BRCA1 alleles

Molecular Oncology, EarlyView.
Synthetic lethality screens performed to identify novel vulnerabilities often model complete gene loss, thereby overlooking patient‐derived hypomorphic mutations. In this study, we have performed genome‐wide CRISPR screens on BRCA1 hypomorphic mutations, showing BRCA1I26A behaves like wild‐type, while BRCA1R1699Q mimics deficiency. Furthermore, we have
Anne Schreuder, Klaas de Lint, Hồ Mỹ Phúc Võ, Mariana M. Góis, Rosalie A. Kampen, Marta San Martin Alonso, Ilse Nootenboom, Veronica Garzero, Tiemen J. Wendel, Rob M. F. Wolthuis, Sylvie M. Noordermeer   +10 more
wiley   +1 more source

Variation in RAD51 details a hub of functions: opportunities to advance cancer diagnosis and therapy [version 1; referees: 4 approved]

F1000Research, 2018
Loss of genome stability is one of the hallmarks of the enabling characteristics of cancer development. Homologous recombination is a DNA repair process that often breaks down as a prelude to developing cancer. Conversely, homologous recombination can be
Nick LL van der Zon, Roland Kanaar, Claire Wyman   +2 more
doaj   +1 more source

Rad54, the motor of homologous recombination [PDF]

DNA Repair, 2010
Homologous recombination (HR) performs crucial functions including DNA repair, segregation of homologous chromosomes, propagation of genetic diversity, and maintenance of telomeres. HR is responsible for the repair of DNA double-strand breaks and DNA interstrand cross-links. The process of HR is initiated at the site of DNA breaks and gaps and involves
Alexander V, Mazin, Olga M, Mazina, Dmitry V, Bugreev, Matthew J, Rossi   +3 more
openaire   +2 more sources

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

FEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada, Mina Iwaki, Kyosuke Hagiri, Rei Izumi, Masahiko Harata, Chihiro Horigome   +5 more
wiley   +1 more source

High Rates of Homologous Recombination in the Mite Endosymbiont and Opportunistic Human Pathogen Orientia tsutsugamushi

, 2010
The study was funded by the Wellcome Trust. PS was supported by grants from the Wellcome Trust (073135) and British Infection Society.Orientia tsutsugamushi is an intracellular alpha-proteobacterium which resides in trombiculid mites, and is the ...
Feil, EJ, Sonthayanon Piengchan, Wuthiekanun Vanaporn, Stuart D Blacksell, Sharon J Peacock, Blacksell, SD, Bentley Stephen D., Sharon J. Peacock, Bentley, SD, Day, Nicholas P. J., Feil Edward J., Day Nicholas P. J., Stephen D. Bentley, Sonthayanon, P, Chierakul, Wirongrong, Stephen D Bentley, Blacksell, Stuart D., Chierakul Wirongrong, Piengchan Sonthayanon, Stuart D. Blacksell, Day, NP, Bentley, Stephen D., Nicholas P J Day, Holden, MT, Wirongrong Chierakul, Mathew T G Holden, Peacock, Sharon J., Feil, Edward J., Wuthiekanun, Vanaporn, Peacock, SJ, Edward J Feil, Wuthiekanun, V, Nicholas P. J. Day, Sonthayanon, Piengchan, Blacksell, Stuart, Vanaporn Wuthiekanun, Edward J. Feil, Chierakul, W, Peacock Sharon J., Day, Nicholas, Holden, Mathew T. G., Holden, Matthew, Blacksell Stuart D., Holden Mathew T. G., Mathew T. G. Holden   +44 more
core   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Guidelines for identifying homologous recombination events in influenza A virus. [PDF]

PLoS ONE, 2010
The rapid evolution of influenza viruses occurs both clonally and non-clonally through a variety of genetic mechanisms and selection pressures. The non-clonal evolution of influenza viruses comprises relatively frequent reassortment among gene segments ...
Maciej F Boni, Menno D de Jong, H Rogier van Doorn, Edward C Holmes   +3 more
doaj   +1 more source