Results 51 to 60 of about 233,309 (305)

Distinct roles for two RAD51-related genes in Trypanosoma brucei antigenic variation

, 2005
In Trypanosoma brucei, DNA recombination is crucial in antigenic variation, a strategy for evading the mammalian host immune system found in a wide variety of pathogens.
Proudfoot, C, McCulloch, Richard, Proudfoot, Christopher, McCulloch, R   +3 more
core   +1 more source

Homologous recombination is unlikely to play a major role in influenza B virus evolution

Virology Journal, 2008
Influenza B viruses cause a significant amount of morbidity and mortality. The occurrence of homologous recombination in influenza viruses is controversial.
Liu Xi-Ping, Han Guan-Zhu, Li Si-Shen
doaj   +1 more source

Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis

FEBS Letters, EarlyView.
Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee, Junho Jeong, Chae Yeong Lee, Hyunjoon Cho, Man Hwan Oh, Je Chul Lee, Jee‐Young Lee, Yangmee Kim   +7 more
wiley   +1 more source

The Influence of Homologous Arm Length on Homologous Recombination Gene Editing Efficiency Mediated by SSB/CRISPR-Cas9 in Escherichia coli

Microorganisms
The precise editing of genes mediated by CRISPR-Cas9 necessitates the application of donor DNA with appropriate lengths of homologous arms and fragment sizes.
Ran Chai, Jiaxiang Guo, Yue Geng, Shuai Huang, Haifeng Wang, Xinding Yao, Tao Li, Liyou Qiu   +7 more
doaj   +1 more source

KDM7A and KDM1A inhibition suppresses tumour promoting pathways in prostate cancer

Molecular Oncology, EarlyView.
Treatment resistance is a major challenge for patients with advanced prostate cancer. This study examined an alternative approach to target the major prostate cancer‐promoting pathway by targeting epigenetic factors, whose levels are higher in tumours.
Jennie N Jeyapalan, Veronika M Metzler, Simone de Brot, Corinne L Woodcock, Anna E Harris, Jennifer Lothion‐Roy, Emeli M Nilsson, Atara Ntekim, Michael S Toss, Jenny L Persson, Francesca Khani, Brian D Robinson, Lorraine J Gudas, Emad Rakha, David M Heery, Catrin S Rutland, Nigel P Mongan   +16 more
wiley   +1 more source

DNA break site at fragile subtelomeres determines probability and mechanism of antigenic variation in African trypanosomes. [PDF]

, 2013
Antigenic variation in African trypanosomes requires monoallelic transcription and switching of variant surface glycoprotein (VSG) genes. The transcribed VSG, always flanked by '70 bp'-repeats and telomeric-repeats, is either replaced through DNA double ...
Glover, Lucy, Horn, David, Horn, David; id_orcid, Lucy Glover (24966), Sam Alsford, David Horn (24969), Alsford, Sam, Lucy Glover, Sam Alsford (24967), David Horn   +9 more
core   +1 more source

RAPID SOUTHERN BLOT PROTOCOL FOR THE ANALYSIS OF TARGETED MUTAGENESIS IN THE MOUSE IGHG1 LOCUS

Scientific Papers Animal Science and Biotechnologies, 2023
The efficiency of homologous recombination relies on the lenght of the homology arms and the chromatin compactation of the locus. In ES cells the immuoglobulin locu is compacted. Thus, the homologous recombination events occur at a very low frequency.
ALENA A. BENCSIK, I. BENCSIK, JANA STĂNCULEȚ, M. BURA   +3 more
doaj  

Cell‐cycle‐specific lesion evolution rather than inhibition of double‐strand‐break repair underpins cisplatin radiosensitization

Molecular Oncology, EarlyView.
We analyze cisplatin–DNA adducts (CDAs) and double‐strand breaks (DSBs) in a cell‐cycle‐dependent manner. We find that CDAs form similarly across all cell cycle phases. DSBs arise only in S‐phase. CDAs might not directly impair DSB repair, but S‐phase DSB lesions evolve in the presence of CDAs and disrupt repair in G2, also causing radiosensitization ...
Ye Qiu, Xixi Lin, Emil Mladenov, Veronika Mladenova, Jürgen Thomale, Ali Sak, Yao Wang, Yunxuan Deng, Eleni Gkika, Martin Stuschke, George Iliakis   +10 more
wiley   +1 more source

ruvA Mutants that resolve Holliday junctions but do not reverse replication forks

, 2008
RuvAB and RuvABC complexes catalyze branch migration and resolution of Holliday junctions (HJs) respectively. In addition to their action in the last steps of homologous recombination, they process HJs made by replication fork reversal, a reaction which ...
Bradley Alison Sylvia, Tsaneva, I, Tsaneva Irina, Bénédicte Michel, Baharoglu Zeynep, Irina Tsaneva, Zeynep Baharoglu, Marie Le Masson, Michel, B, Bradley, AS, Baharoglu, Z, Le Masson Marie, Michel Bénédicte, Alison Sylvia Bradley, Le Masson, M   +14 more
core   +1 more source

Assembly and dynamics of the bacteriophage T4 homologous recombination machinery

Virology Journal, 2010
Homologous recombination (HR), a process involving the physical exchange of strands between homologous or nearly homologous DNA molecules, is critical for maintaining the genetic diversity and genome stability of species.
Morrical Scott W, Liu Jie
doaj   +1 more source