Results 31 to 40 of about 210,282 (254)

Mutational Effects of Mobile Introns on the Mitochondrial Genomes of Metschnikowia Yeasts

Frontiers in Genetics, 2021
It has been argued that DNA repair by homologous recombination in the context of endonuclease-mediated cleavage can cause mutations. To better understand this phenomenon, we examined homologous recombination following endonuclease cleavage in a native ...
Scout R. L. Thompson, Dong Kyung Lee, Marc-André Lachance, David Roy Smith   +3 more
doaj   +1 more source

Using single loxP sites to enhance homologous recombination: ts mutants in Sec1 of Dictyostelium discoideum.

PLoS ONE, 2007
BackgroundDictyostelium discoideum amoebae are haploid and, as they share many features with animal cells, should be an ideal creature for studying basic processes such as cell locomotion.
Mark S Bretscher, Margaret Clotworthy
doaj   +1 more source

The anaphase promoting complex impacts repair choice by protecting ubiquitin signalling at DNA damage sites

Nature Communications, 2017
The choice between homologous recombination and non-homologous end-joining is largely influenced by cell cycle. Here the authors show that APCCdh1 promotes homologous recombination by removing USP1, allowing polyubiquitinated histones to recruit BRCA1.
Kyungsoo Ha, Chengxian Ma, Han Lin, Lichun Tang, Zhusheng Lian, Fang Zhao, Ju-Mei Li, Bei Zhen, Huadong Pei, Suxia Han, Marcos Malumbres, Jianping Jin, Huan Chen, Yongxiang Zhao, Qing Zhu, Pumin Zhang   +15 more
doaj   +1 more source

Measuring Homologous Recombination Rates between Chromosomal Locations in Salmonella

Bio-Protocol, 2019
Homologous recombination between two similar DNA molecules, plays an important role in the repair of double-stranded DNA breaks. Recombination can occur between two sister chromosomes, or between two locations of similar sequence identity within the same
Gerrit Brandis, Sha Cao, Diarmaid Hughes   +2 more
doaj   +1 more source

Plant DNA Recombinases: A Long Way to Go

Journal of Nucleic Acids, 2010
DNA homologous recombination is fundamental process by which two homologous DNA molecules exchange the genetic information for the generation of genetic diversity and maintain the genomic integrity.
Rajani Kant Chittela, Jayashree K. Sainis   +1 more
doaj   +1 more source

Replication dynamics of recombination-dependent replication forks

Nature Communications, 2021
Replication forks that are stalled at obstacles on the DNA template can be restarted by homologous recombination. Here, the authors show replication dynamics during homologous recombination-dependent replication fork restart by combining polymerase usage
Karel Naiman, Eduard Campillo-Funollet, Adam T. Watson, Alice Budden, Izumi Miyabe, Antony M. Carr   +5 more
doaj   +1 more source

Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis

FEBS Letters, EarlyView.
Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee, Junho Jeong, Chae Yeong Lee, Hyunjoon Cho, Man Hwan Oh, Je Chul Lee, Jee‐Young Lee, Yangmee Kim   +7 more
wiley   +1 more source

Homologous recombination is unlikely to play a major role in influenza B virus evolution

Virology Journal, 2008
Influenza B viruses cause a significant amount of morbidity and mortality. The occurrence of homologous recombination in influenza viruses is controversial.
Liu Xi-Ping, Han Guan-Zhu, Li Si-Shen
doaj   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

The Influence of Homologous Arm Length on Homologous Recombination Gene Editing Efficiency Mediated by SSB/CRISPR-Cas9 in Escherichia coli

Microorganisms
The precise editing of genes mediated by CRISPR-Cas9 necessitates the application of donor DNA with appropriate lengths of homologous arms and fragment sizes.
Ran Chai, Jiaxiang Guo, Yue Geng, Shuai Huang, Haifeng Wang, Xinding Yao, Tao Li, Liyou Qiu   +7 more
doaj   +1 more source