Results 141 to 150 of about 660,490 (319)

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Decreased cold‐sensing function of the transient receptor potential channel TRPM8 from tailed amphibians

FEBS Open Bio, EarlyView.
Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao, Daiki Kobayashi, Shogo Hori, Osamu Saitoh   +3 more
wiley   +1 more source

Studies on Regioselective Binding Mode of Steroid Molecules in Homology Modeled Cytochrome P450-2C11 [PDF]

, 2009
In this study, we investigated the regioselective binding mode of steroid molecules and structure requirements for steroid molecules for 16[alpha]-hydroxylation by Cytochrome P450-2C11.
Morio Yamada, Eiichi Akaho, Hamed I. Ali, Hukihisa Fujita   +3 more
core   +1 more source

Ecomorphology and history of a recurrent evolutionary adaptation to long-distance bird dispersal in Asteraceae

Boletín de la Sociedad Argentina de Botánica
Background and aims: Genera of Asteraceae, belonging to different tribes, present a repeated pattern of morphological adaptations to epiornithochory and have unique distributions (widely disjunct and/or inhabit remote islands). This pattern requires the
Liliana Katinas, Florencia D. Dosil Hiriart, Jorge V. Crisci   +2 more
doaj   +1 more source

Pharmacological inhibition of the PERK pathway modulates hepatocellular carcinoma growth and immune signaling

FEBS Open Bio, EarlyView.
Pharmacological inhibition of PERK in a DEN‐induced mouse model of liver cancer does not reduce tumor burden but alters cellular stress signaling. Despite blocking PERK activity, downstream stress responses, including CHOP expression, remain active, suggesting compensatory mechanisms within the unfolded protein response that may influence tumor ...
Ada Lerma‐Clavero, Maria Kopsida, Nathalie Arendt, Hans Lennernäs, Markus Sjöblom, Femke Heindryckx   +5 more
wiley   +1 more source

GeneWeld: a method for efficient targeted integration directed by short homology [PDF]

, 2018
Choices for genome engineering and integration involve high efficiency with little or no target specificity or high specificity with low activity. Here, we describe a targeted integration strategy, called GeneWeld, and a vector series for gene tagging ...
Carlson, Daniel, McGrail, Maura, Wierson, Wesley, McKeighan, Kenna, MIng, Zhitao, Haltom, Jeffrey, Welker, Jordan, Mann, Carla, Mikelson, Christopher, Almeida, Maira, Clark, Karl, Webber, Beau, Wehmeier, Alec, Chien, Chi-­Bin, Essner, Jeffrey, Webster, Dennis, Ekker, Stephen, Lan, Merrina, Weiss, Trevor, Kwan, Kristen, Moriarity, Branden, Torrie, Melanie, Solin, Staci, Vollbrecht, Macy, Balciunas, Darius, Dobbs, Drena   +25 more
core   +1 more source

A general theory of the complex pronotum morphology of treehoppers in Smiliinae and its relatives (Hemiptera: Membracidae) and its applicability to other subfamilies

European Journal of Entomology
Treehoppers are characterized by exaggerated and three-dimensionally modified pronotal structures, known as "helmets". The treehopper pronotum has been studied mainly in terms of taxonomic diagnosis for over a century.
Kanta SUGIURA, Tensho TERANO, Haruhiko ADACHI, Jin HAGIWARA, Keisuke MATSUDA, Kenji NISHIDA, Paul HANSON, Shigeru KONDO, Yasuhiko CHIKAMI, Hiroki GOTOH   +9 more
doaj   +1 more source

A yeast model of 5‐oxoproline accumulation reveals a general toleration to 5‐oxoproline

FEBS Open Bio, EarlyView.
Using a yeast model, we show that even high accumulation of 5‐oxoproline causes only mild cellular stress and does not trigger oxidative stress. Instead, cells adapt by activating efflux pumps and diverse protective pathways, suggesting that previously proposed harmful effects of 5‐oxoproline may arise from indirect metabolic imbalances rather than the
Pratiksha Dubey, Vaishnavi Sanjayan, Praveen Singh, Shantanu Sengupta, Anand Kumar Bachhawat   +4 more
wiley   +1 more source

Proteomic Profiling of Bacterial Extracellular Vesicles for Exploring Ovarian Cancer Biomarkers

Journal of Extracellular Biology
Extracellular vesicles (EVs) are present in body fluids and act as disease biomarkers. Emerging evidence has proven that EVs are released not only from mammalian cells but also from bacteria.
Eri Asano‐Inami, Akira Yokoi, Kosuke Yoshida, Kentaro Taki, Masami Kitagawa, Kazuhiro Suzuki, Ryosuke Uekusa, Yukari Nagao, Nobuhisa Yoshikawa, Kaoru Niimi, Yusuke Yamamoto, Hiroaki Kajiyama   +11 more
doaj   +1 more source

One size does not fit all: An in vitro evaluation of the effects of bezafibrate and medroxyprogesterone acetate on human SH‐SY5Y and U‐87 MG cancer cells

FEBS Open Bio, EarlyView.
Drugs previously repurposed to target blood cancers reduced neuroblastoma and glioblastoma cell growth and viability. However, their levels of anticancer activity were different and their clinical application may be problematic due to side effects at effective doses.
Abhishek Kharawatkar, Francesco Michelangeli, Farhat Latif Khanim, Christopher Martin Bunce, William Eustace Basil Johnson   +4 more
wiley   +1 more source