NewN-Glycans in Horseradish Peroxidase
We describe here the structures of several new N-glycan oligosaccharides from horseradish peroxidase (HRP). Glycopeptides from HRP were digested with glycoamidase A (from sweet almond) to release the N-glycans. The oligosaccharides were reductively aminated with 2-aminopyridine, and separated by high-performance liquid chromatography on octadecylsilyl-
N, Takahashi +5 more
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Functionalized Palladium Nanoparticles for Hydrogen Peroxide Biosensor
We present a comparison between two biosensors for hydrogen peroxide (H2O2) detection. The first biosensor was developed by the immobilization of Horseradish Peroxidase (HRP) enzyme on thiol-modified gold electrode.
H. Baccar, T. Ktari, A. Abdelghani
doaj +1 more source
Cobalt-substituted horseradish peroxidase.
Horseradish peroxidase can be reconstituted with cobalt porphyrin to give a cobaltic holoenzyme having physicochemical properties quite similar to those of the native ferric protein. The cobaltic protein (Co3+HRP) can be reduced to the cobaltous form (CoHRP), the analogue of ferroperoxidase and the reduced cobalt protein can bind O2 to form an analogue
M Y, Wang, B M, Hoffman, P F, Hollenberg
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Immobilization of peroxidase from fresh horseradish extract for anthraquinone dye decolorization
Synthetic dyes are used in all sectors of industry and represent a serious ecological problem. By the presence, anthraquinone dyes are right after the azo dyes.
Nataša Ž. Šekuljica +6 more
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Effect of a Conical Cellulose Structure on Horseradish Peroxidase Biomacromolecules
The effect of a dielectric conical structure on the adsorption properties of an enzyme on mica was studied by atomic force microscopy (AFM) with the example of horseradish peroxidase (HRP). The cone used was a cellulose cone with a 60° apex angle. Namely,
Yuri D. Ivanov +10 more
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Electrochemical Detection of Prostate Carcinoma Biomarkers Using Nanotechnology [PDF]
The first chapter of this thesis speaks about prostate specific antigen, carbon nanotubes and horseradish peroxidase. The second chapter discusses the electrochemistry and catalysis of horseradish peroxidase (HRP) and myoglobin (Mb) covalently attached ...
Leonard, Kathryn
core +1 more source
Differential Input from the Amygdaloid Body to the Ventromedial Hypothalamic Nucleus in the Rat [PDF]
Differential amygdaloid afferents to anterior dorsal, anterior ventral, posterior dorsal and posterior ventral subdivisions of the ventromedial hypothalamic nucleus (VMH) were studied by means of retrograde transport of horseradish peroxidase (HRP ...
Fukuda, M., +3 more
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Conjugation of Antibodies to Horseradish Peroxidase [PDF]
Antibodies conjugated with horseradish peroxidase (HRP) are one of the most widely used bioreagents in the biological sciences. This protocol is a basic method for adding HRP to a thiolated antibody and can be adapted for use with different cross-linkers. Conjugation methods usually focus on linking through the lysines on HRP because there are only six
Eric A, Berg, Jordan B, Fishman
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Comparison of two peroxidases with high potential for biotechnology applications – HRP vs. APEX2
Peroxidases are essential elements in many biotechnological applications. An especially interesting concept involves split enzymes, where the enzyme is separated into two smaller and inactive proteins that can dimerize into a fully active enzyme.
Sanja Škulj +7 more
doaj +1 more source
WEAK-ASSOCIATED WITH CELL WALL PEROXIDASE DURING THE ROOT INFECTION [PDF]
The role of weak-associated potato cell wall peroxidase in potato resistance to ring rot pathogen is shown. The data obtained showed that an addition of anti-horseradish peroxidase antibodies to resistant to this pathogen potato cv Lugovskoy cell culture
Graskova I.A. +4 more
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