Results 41 to 50 of about 131,708 (265)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Devising Strain Hardening Models Using Kocks–Mecking Plots—A Comparison of Model Development for Titanium Aluminides and Case Hardening Steel

Metals, 2016
The present study focuses on the development of strain hardening models taking into account the peculiarities of titanium aluminides. In comparison to steels, whose behavior has been studied extensively in the past, titanium aluminides possess a much ...
Markus Bambach, Irina Sizova, Sebastian Bolz, Sabine Weiß   +3 more
doaj   +1 more source

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

FEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source

Dissolution and Precipitation Behavior for Hot Forming of 7021 and 7075 Aluminum Alloys

Metals, 2018
Due to their high strength, 7xxx aluminum sheets are increasingly used for structural automobile components. One of the major challenges is the formability of these alloys during the production process while retaining high strengths in service.
Benjamin Milkereit, Martin Österreich, Philipp Schuster, Georg Kirov, Ermal Mukeli, Olaf Kessler   +5 more
doaj   +1 more source

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source

Natural Products as Geroprotective Modulators in Diabetic Nephropathy: A Mechanistic Framework Integrating Aging Hallmarks and the AMPK–SIRT1–Nrf2 Axis

Aging and Cancer, EarlyView.
Natural products target the aging kidney in diabetic nephropathy by restoring the AMPK–SIRT1–Nrf2 axis, reducing oxidative stress, inflammation, fibrosis, and cellular senescence while enhancing mitochondrial biogenesis and antioxidant defenses.
Sherif Hamidu, Latif Adams, Eunice Ampem‐Danso, Justice Kumi, Abigail Aning, Fatima Hamidu, Mustapha Abdul Mumin Mohammed, Ayuba Nii Darku Ankrah, Seth Kwabena Amponsah   +8 more
wiley   +1 more source

Development of a partition panel of an Al6061 sheet metal part for the improvement of formability and mechanical properties by hot forming quenching

Advances in Mechanical Engineering, 2017
In this study, the hot forming quenching process was investigated to improve the deficiencies that arise in materials subjected to conventional cold stamping, such as low formability and undesirable mechanical properties.
Dae-Cheol Ko, Dae-Hoon Ko, Jae-Hong Kim, Joon-Hong Park   +3 more
doaj   +1 more source

Epilepsy‐Associated Variants of a Single SCN1A Codon Exhibit Divergent Functional Properties

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Pathogenic variants in SCN1A, which encodes the voltage‐gated sodium channel NaV1.1, are associated with multiple epilepsy syndromes exhibiting a range of clinical severity. SCN1A variants are reported in different syndromes, including Dravet syndrome, which is associated with loss‐of‐function, whereas neonatal/infantile‐onset ...
Lanie N. Liebovitz, Christopher H. Thompson, Linda C. Laux, Alfred L. George Jr.   +3 more
wiley   +1 more source

Hot forming recrystallization kinetics in steel [PDF]

Metalurgija, 2010
The theory of kinetics of static recrystallization of steel during hot forming links the phenomenon to certain critical strain, grain size, strain rate, activation energy and temperature. The basic description is provided by the Avrami equation.
J. Kliber, R. Fabík, I. Vitez, K. Drozd
doaj  

Hot forming of large spur gears

Procedia Engineering, 2017
Abstract Forming processes are generally characterized by a high degree of material utilization as well as short process times and, consequently, a decent economic efficiency. Considering their application in the manufacturing of large spur gears, forming processes offer a significantly attractive characteristic for the production of essential ...
Dirk Landgrebe, Andreas Sterzing, Mike Lahl, Matthias Milbrandt, Anne Manuela Taubert   +4 more
openaire   +1 more source