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Study on LOD of Chinese Honey Adulterated with HFCS by HPAEC-PAD

Advanced Materials Research, 2013
In consideration of the adulterated situation and characteristics of Chinese honey market, high performance anion exchange chromatography-pulsed amperometric detection (HPAEC-PAD) used for detection of high fructose corn syrup (HFCS) in adulterated honey were further studied in this paper, Isocratic elution with optimize chromatographic conditions, the
Jin Zhe He, Ming Lei, Pei Long Sun
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Action Pattern of a Novel G-Specific Alginate Lyase: Determination of Subsite Specificity by HPAEC–PAD/MS

Journal of Agricultural and Food Chemistry, 2023
G-specific alginate lyases are important tools for alginate fragment biodegradation and oligosaccharide production, which have great potential in alginate refining research. In this research, a novel G-specific alginate lyase Aly7Ce was cloned, expressed, and characterized, with the optimal reaction conditions at 30 °C and pH 8.0.
Jiajing Li   +6 more
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Exploratory SAXS and HPAEC-PAD studies of starches from diverse plant genotypes

Carbohydrate Polymers, 2006
A substantial amount of effort has been expended on developing a suitable model to describe the small angle X-ray scattering (SAXS) profiles of starch. The modelling work now offers good descriptions of the fine structure of the starch granule, with recent advances [Daniels, D.R., & Donald, A.M. (2003).
Sanderson, James S.   +4 more
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HPAEC-PAD quantification of Haemophilus influenzae type b polysaccharide in upstream and downstream samples

Vaccine, 2015
Due to the rapidly increasing introduction of Haemophilus influenzae type b (Hib) and other conjugate vaccines worldwide during the last decade, reliable and robust analytical methods are needed for the quantitative monitoring of intermediate samples generated during fermentation (upstream processing, USP) and purification (downstream processing, DSP ...
Robert M.F. van der Put   +4 more
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Carbohydrate composition analysis of bacterial polysaccharides: Optimized acid hydrolysis conditions for HPAEC-PAD analysis

Analytical Biochemistry, 1992
The capsular polysaccharide from Haemophilus influenzae type b (polyribosyl ribitol-phosphate; PRP) and the capsular polysaccharides from Streptococcus pneumoniae types 6B, 14, 18C, and 23F (Pn6B, Pn14, Pn18C, and Pn23F) were subjected to acid hydrolysis using hydrofluoric (HF) and/or trifluoroacetic acid (TFA) and high-pH anion-exchange chromatography
C C, Ip   +3 more
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Validated HPAEC-PAD method for prebiotics determination in synbiotic fermented milks during shelf life

Analytical and Bioanalytical Chemistry, 2010
Interest concerning functional food has been growing in recent years, and much attention has been focused on the choice of prebiotic fibers and probiotic microorganisms added to food products with the aim of improving health, producing synbiotic products.
C. Borromei   +6 more
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Trehalose determination in linseed subjected to osmotic stress. HPAEC‐PAD analysis: an inappropriate method

Physiologia Plantarum, 2012
Trehalose is a non‐reducing disaccharide involved in stress tolerance in plants. To understand better the role of trehalose in the osmotic stress response in linseed (Linum usitatissimum), trehalose content in leaves was studied. First, the method commonly used for sugar determination, high performance anion exchange chromatography with pulsed ...
Quéro, Anthony   +8 more
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Quantitative Analysis of Debranched Amylopectin by HPAEC-PAD with a Postcolumn Enzyme Reactor

Journal of Liquid Chromatography & Related Technologies, 1997
Abstract The chain-length distribution of debranched amylopectin was analyzed by using high performance anion-exchange chromatography with an amyloglucosidase (AMG) reactor and a pulsed amperometric detector (HPAEC-ENZ-PAD). The effects of reactor length, reactor temperature, and additional a-amylase reactor were studied. Results showed that a 2 mm i.d.
K. S. Wong, J. Jane
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Neutral-sugar determination by HPAEC-PAD to characterise polysaccharide degradation by colonic bacteria

Zeitschrift f�r Lebensmitteluntersuchung und -Forschung A, 1997
In vitro methods are widely used to characterise dietary fibre fermentability in the human hind gut. The activity of faecal bacteria can be followed by measuring fermentation products and substrate degradation. This paper describes a method for establishing neutral-sugar profiles by HPAEC-PAD during in vitro fermentation studies.
Vincent Lebet   +2 more
openaire   +1 more source

Quantitative analysis of intracellular sugar phosphates and sugar nucleotides in encapsulated streptococci using HPAEC‐PAD

Biotechnology Journal, 2009
AbstractMetabolomics is a powerful tool for the study of biological systems. Besides analytical techniques, cell harvest and extraction are critical steps, especially when studying encapsulated streptococci. We have compared four different harvesting techniques for biomass from liquid culture of the hyaluronic acid (HA)‐producing bacterium ...
Marcellin, Esteban   +3 more
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