Results 61 to 70 of about 1,055,689 (337)

Method Validation and Characterization of Red Pigment in Beta vulgaris Peels and Pomaces by HPLC‐UV and UHPLC‐MS/MS [PDF]

, 2022
Vincent Rotich, Phanice Wangila, Jackson K. Cherutoi   +2 more
openalex   +1 more source

Hands‐on protocol for preparing water‐soluble fractions from agri‐food samples for NMR‐based metabolomics analysis

FEBS Open Bio, EarlyView.
This research protocol outlines a workflow for nuclear magnetic resonance (NMR)‐based metabolomics in the agri‐food sector. Using two case studies—strawberry leaves (solid matrix) and wine (liquid matrix)—it details the procedures for sample preparation, data acquisition, and processing.
Andrea Fernández‐Veloso, Jaime Hiniesta‐Valero, Alejandra Guerra‐Castellano, Miguel A. De la Rosa, Irene Díaz‐Moreno   +4 more
wiley   +1 more source

Development and Validation of a Bioanalytical Method for Estimating Ellagic Acid in Wistar Rat Plasma by RP-HPLC Using a Gradient Elution Technique

BIO Integration
Background: Ellagic acid (EA) is a natural polyphenolic compound recognized for bioactive and pharmacologic properties that is found abundantly in various plant groups, particularly eudicotyledons.
Varsha Mane, Nagesh Aloorkar, Vishwajeet Ghorpade, Taturang Shinde, Avinash Kolekar, Sandip Gore   +5 more
doaj   +1 more source

Development and Validation of an Improved HPLC-UV Method for the Determination of Tildipirosin in Horse Plasma

Acta Veterinaria, 2022
A simple, rapid, low-cost, and sensitive high-performance liquid chromatographic method was developed to determine tildipirosin in horse plasma. Plasma samples were extracted with diethyl ether, and after evaporation, tildipirosin was determined by ...
Galecio Juan Sebastián, Hernandis Verónica, Escudero Elisa, Marín Pedro   +3 more
doaj   +1 more source

Engineering tandem VHHs to target different epitopes to enhance antibody‐dependent cell‐mediated cytotoxicity

FEBS Open Bio, EarlyView.
Tandem VHH targeting distinct EGFR epitopes were engineered into a monovalent bispecific antibody (7D12‐EGA1‐Fc) with more potent ADCC without increasing affinity to EGFR. Structural modeling of 7D12‐EGA1‐Fc showed cross‐linking of separate EGFR domains to enhance CD16a engagement on NK cells.
Yuqiang Xu, Hao Jiang, Limin Chen, Fulai Zhou, Ying Jin, Mark L. Chiu   +5 more
wiley   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Unlocking the Power of Quercetin‐Encapsulated Mesoporous Bioactive Glass Nanoparticles: A Multifunctional Approach to Bone Regeneration

Advanced Engineering Materials, EarlyView.
Mesoporous bioactive glass nanoparticles (MBGNs) are investigated for bone regeneration given their remarkable structural and functional properties. MBGNs are functionalized with Mn and Cu and incorporated with quercetin, a natural flavonoid exhibiting antioxidant, anti‐inflammatory, and antimicrobial properties.
Giovanni Lo Bello, Qaisar Nawaz, Pier Francesco Ferrari, Laura Pastorino, Roberto Raiteri, Aldo R. Boccaccini   +5 more
wiley   +1 more source

Effects of TCMs and Lactobacillus strains on immunosuppressed mice and bacteriostatic effect on Escherichia coli K88 after fermentation

Biotechnology & Biotechnological Equipment, 2019
The aim of this study was to investigate a novel means of treating piglet diarrhoea caused by enterotoxigenic Escherichia coli (ETEC) strain K88 using traditional Chinese medicine (TCM) fermented by selected probiotic strains.
Wei Gu, Mingjie Sun, Wentao Yang, Lirong Wang, Yanlong Jiang, Chunfeng Wang, Guilian Yang   +6 more
doaj   +1 more source

Variable major proteins of Borrelia hermsii. Epitope mapping and partial sequence analysis of CNBr peptides. [PDF]

, 1985
The variable major proteins (VMP) of serotypes 7 and 21 of the relapsing fever agent Borrelia hermsii were isolated by detergent extraction and high performance liquid chromatography.
Barbour, AG, Barstad, PA, Coligan, JE, Raum, MG   +3 more
core  

Influence of the sebaceous gland density on the stratum corneum lipidome [PDF]

, 2018
The skin surface lipids (SSL) result from the blending of sebaceous and epidermal lipids, which derive from the sebaceous gland (SG) secretion and the permeability barrier of the stratum corneum (SC), respectively.
Camera, Emanuela, Kozul, Nina, Ludovici, Matteo, Materazzi, Stefano, Picardo, Mauro, Risoluti, Roberta   +5 more
core   +1 more source