High-speed countercurrent chromatography (HSCCC) is a leading method for the fast separation of natural products from plants. It was used for the preparative isolation of two flavone monoglucosides present in the capitula of Eriocaulon ligulatum (Vell ...
L.C. Santos +6 more
doaj +2 more sources
Target-Guided Isolation and Purification of Antioxidants from Urtica laetevirens Maxim. by HSCCC Combined with Online DPPH-HPLC Analysis. [PDF]
Urtica laetevirens Maxim. is used extensively in traditional Chinese medicine (TCM) for its potent antioxidative properties. In this study, three antioxidants were purified from U. laetevirens. using HSCCC guided by online DPPH-HPLC analysis.
Li A +9 more
europepmc +2 more sources
Antimycobacterial and Nitric Oxide Production Inhibitory Activities of Goyazensolide and Centratherin Isolated From Eremanthus crotonoides by High-Speed Countercurrent Chromatography. [PDF]
ABSTRACT In this study, bioassay‐guided fractionation of the ethanolic extract from Eremanthus crotonoides leaves enabled the isolation of two bioactive sesquiterpene lactones, centratherin and goyazensolide, which were evaluated for their antimycobacterial and anti‐inflammatory properties.
da Rocha Ximenes NG +7 more
europepmc +2 more sources
High-speed countercurrent chromatography (HSCCC) is a liquid-liquid separation chromatographic technique which uses no solid supporting medium. During its rapid development in the last 30 years, great progress has been made in the instrumentation, the ...
Yao Shun +4 more
doaj +2 more sources
Advances in Eco-Friendly Extraction of Fruit Bioactive Compounds: Technologies, Challenges and Future Directions. [PDF]
Fruit bioactive compounds offer antioxidant, anti‐inflammatory, and preventive benefits, yet extraction is hindered by poor solubility and conventional methods using toxic solvents and high energy. This review evaluates traditional and advanced eco‐friendly technologies, highlighting efficiency, selectivity, and sustainability.
Hndeya AG, Sbhatu DB, Gebreyohannes G.
europepmc +2 more sources
Separation of Phenylpropanoid Glycosides from a Chinese Herb by HSCCC [PDF]
An effective high-speed counter-current chromatography method was established for the preparative isolation and purification of two phenylpropanoid glycosides from the Tibetan medicinal plant Pedicularis longiflora Rudolph. var. tubiformis (Klotz). Tsoong.
Chen, Chen +4 more
openaire +2 more sources
Separation of Four Phenylpropanoid Glycosides from a Chinese Herb by HSCCC [PDF]
Four phenylpropanoid glycosides (PPGs), such as echinacoside, wiedemannioside C, forsythoside B and verbascoside, were isolated and purified from the Tibetan medicinal herb Pedicularis longiflora Rudolph. var. tubiformis (Klotz) Tsoong by high-speed counter-current chromatography (HSCCC) combined with macroporous resin (MR) column separation for the ...
Lin, Zhang +4 more
openaire +2 more sources
Discrimination and Experimental Study of HSCCC Operational Modal based on LSCF
Abstract The dynamic characteristics of high-speed counter-current chromatography (HSCCC) have a significant impact on its separation efficiency and equipment stability. The accurate discrimination of the dynamic characteristic during the separation process constitutes the prerequisite for clarifying the separation mechanism and ...
Qin Yin +3 more
openaire +1 more source
HSCCC Separations of Rutin Esters Obtained by Enzymatic Reaction Catalyzed by Lipase
The flavonoid rutin presents several pharmacological effects, despite this, its application in the pharmaceutical industry can be significantly limited by its low bioavailability. The development of lipophilic derivatives by esterification of hydroxyl groups with fatty acid chains may be an effective strategy to change their physicochemical properties.
Jéssyca Couto +8 more
openaire +3 more sources
Separation of Cyclosporins andOther Antibiotics by HSCCC [PDF]
Applications of high-speed counter-current chromatography (HSCCC) for the separations of antibiotics, cyclosporins and isoflavones, by a marine Micromonospora and macrolides including tacrolimus, ascomycin, and dihydroFk-506 are described. The application of silver ion HSCCC technique in the separation of tacrolimus from its components is also ...
openaire +2 more sources

