Results 191 to 200 of about 145,825 (238)

Author Correction: Viral entry shapes HCMV latency establishment. [PDF]

open access: yesNat Commun
Kitsberg Y   +8 more
europepmc   +1 more source

Room‐temperature crystal structure of a metal‐dependent W‐formate dehydrogenase by serial synchrotron crystallography

open access: yesActa Crystallographica Section D, EarlyView.
We report a room‐temperature serial synchrotron X‐ray crystallographic structural analysis of Nitratidesulfovibrio vulgaris formate dehydrogenase AB, an enzyme that reduces CO2 to formate.Metal‐dependent formate dehydrogenases (Fdhs) are a promising research target in efforts to mitigate climate change by developing active, efficient, selective and ...
Guilherme Vilela-Alves   +7 more
wiley   +1 more source

Avoiding pitfalls when modelling ligands in macromolecular crystallography

open access: yesActa Crystallographica Section D, EarlyView.
Flawed protein–ligand X‐ray structures can misdirect drug discovery based on PDB‐deposited models. This review uses publicly available PDB examples to highlight potential pitfalls, from absent ligands to incorrect chemistry, and presents a practical validation approach to improve model reliability.The determination of protein–ligand complex structures ...
Oliver S. Smart   +4 more
wiley   +1 more source

Direct deposition on pre‐cooled grids: a new approach to sub‐millisecond cryoEM grid preparation

open access: yesActa Crystallographica Section D, EarlyView.
We describe a new method to prepare grids for cryoEM on the sub‐millisecond timescale.There has been a rapid expansion in new approaches to sample preparation for single‐particle cryoEM. One drive has been around speeding up the process both for the application of time‐resolved studies and in outrunning some of the negative effects which may come from ...
David P. Klebl   +5 more
wiley   +1 more source

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