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Giant oversights in the human gut virome
Gut, 2019We read with interest the paper by Zuo et al ,1 which used deep sequencing to identify gut mucosal virome alterations in individuals with UC. One of many interesting findings reported by the authors was the detection of giant viruses infecting algae and amoeba ( Mimivirus and Chrysochromulina ericina virus).
Thomas D S Sutton +2 more
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Direct sequencing of human gut virome fractions obtained by flow cytometry [PDF]
The sequence assembly of the human gut virome encounters several difficulties. A high proportion of human and bacterial matches is detected in purified viral samples. Viral DNA extraction results in a low DNA concentration, which does not reach the minimal limit required for sequencing library preparation. Therefore, the viromes are usually enriched by
Maria Džunková +2 more
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Metagenome of human gut virome and human gut microbiota
2022Metagenome of human gut virome and human gut ...
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Authors response: giant oversights in the human gut virome
Gut, 2019We explored gut mucosal virome alterations in UC1 and appreciate the insightful comments from the authors who highlighted some of the important limitations of human gut virome studies.2 Unlike the bacteria microbiome, the gut virome remains largely underexplored.
Tao Zuo, Siew C Ng
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Another piece of puzzle for the human microbiome: the gut virome under dietary modulation
The virome is the most abundant and highly variable microbial consortium in the gut. Because of difficulties in isolating and culturing gut viruses and the lack of reference genomes, the virome has remained a relatively elusive aspect of the human microbiome.
Jinfeng Wang, Fengxiang Zhao
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A database to identify the human gut virome
Nature Microbiology, 2022Oluwasegun I. Daramola +1 more
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Metagenomic Analyses of the Human Gut Virome
2013The human gut harbors tens of trillions of microbes belonging to all three domains of life, Bacteria, Archaea, and Eukarya; most are members of Bacteria. These organisms collaborate and compete for functional niches and physical space: habitats), together forming a continuously functioning metabolic organ that influences many aspects of host biology ...
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On bioinformatics of the human gut virome
2022Shotgun metagenomics has revealed an unprecedented diversity of viruses maintaining interactions with organisms from all divisions of life. Thus, metagenomics changed the perspective on viruses: from mere pathogens to significant players within ecological systems.
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Viral Genome Isolation from Human Faeces for Succession Assessment of the Human Gut Virome
2018Despite the important role of the microbiota in the human gastrointestinal tract (GIT) and its impact on life-long health, the successional process through which this microbial community develops during infancy is still poorly understood. Specially, little is known about how the amount and type of viruses present in the GIT, i.e., the virome, varies ...
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