Results 81 to 90 of about 418,253 (268)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

A Hybrid Software Architecture Evaluation Method for Dynamic System Development Method

The Nucleus, 2016
 Quality is an important paradigm while delivering software. Past traditional models have many problems. Agile process models overcome these problems, but these models are facing many challenges. The main challenge is the absence of proper Software Architecture Evaluation Method (SAEM) for agile models.
R. Awan, S. S. Muhammad, M. A. Fahiem, S. Awan   +3 more
openaire   +1 more source

Self‐Regulated Learning Meets AI: Reinterpreting Self‐Regulation, Co‐Regulation, and Socially Shared Regulation in Human–AI Interaction

New Directions for Adult and Continuing Education, EarlyView.
ABSTRACT Advancing artificial intelligence (AI) has transformed learning and work, yet higher education and professional development programs have not systematically equipped learners for AI‐prevalent environments. This lack of preparation creates uncertainty regarding control, responsibility, trust, and accountability.
Moon‐Heum Cho, Jerusalem Merkebu
wiley   +1 more source

The Innovative Directions In Development And Implementations Of Hybrid Technologies In Surface Engineering

Archives of Metallurgy and Materials, 2015
The dynamics of the development of modern technology depends, to a great extent, on the possibilities of producing the innovative materials with high functionality parameters, which could be used in modern, highly advanced technological processes ...
Mazurkiewicz A., Smolik J.
doaj   +1 more source

Sex Representation in US Stroke Clinical Trials: A Decade of Trends and Challenges

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Stroke remains a major cause of disability and mortality in the US, with significant sex‐based disparities, and females remain underrepresented in stroke clinical trials. We aimed to examine sex representation in US‐based stroke clinical trials, identify trial characteristics associated with higher female enrollment (≥ 50%), and ...
Chaitali Dagli, Mudasir Andrabi, Tova Cohen, Pranali G. Patel, Tapasya Katta, Chen Lin   +5 more
wiley   +1 more source

Exploring the Strategies of Male Sterility for Hybrid Development in Hexaploid Wheat: Prevailing Methods and Potential Approaches

Rice
Hybrid breeding has emerged as a pivotal strategy to enhance wheat crop yield, a critical step to meet the escalating food demand for the growing global population.
Muhammad Abdullah, Usama Sheraz, Arisha Tul Ain, Bisma Nasir, Sardar Hammad, Sajid Shokat   +5 more
doaj   +1 more source

Design of Hybrid RNA Polymerase III Promoters for Efficient CRISPR-Cas9 Function

Bio-Protocol, 2018
The discovery of the CRISPR-Cas9 system from Streptococcus pyogenes has allowed the development of genome engineering tools in a variety of organisms. A frequent limitation in CRISPR-Cas9 function is adequate expression levels of sgRNA.
Joshua Misa, Cory Schwartz, Ian Wheeldon
doaj   +1 more source

Pultrusion manufacturing process development: Cure optimization by hybrid computational methods

Computers & Mathematics with Applications, 2007
AbstractThis paper develops a hybrid approach, based on genetic algorithms and simplex method, to optimize the accuracy of a manufacturing process by material pultrusion. The numerical model, proposed in a recent paper of the same authors, is solved by a finite difference scheme.
CARLONE, PIERPAOLO, PALAZZO, Gaetano Salvatore, PASQUINO, Raimondo   +2 more
openaire   +1 more source

Comparing the Effect of Semi‐Immersive Virtual Reality, Computerized Cognitive Training, and Traditional Rehabilitation on Cognitive Function in Multiple Sclerosis: A Randomized Clinical Trial

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Background Cognitive impairment is a common non‐motor symptom in Multiple Sclerosis (MS), negatively affecting autonomy and Quality of Life (QoL). Innovative rehabilitation strategies, such as semi‐immersive virtual reality (VR) and computerized cognitive training (CCT), may offer advantages over traditional cognitive rehabilitation (TCR ...
Maria Grazia Maggio, Desiree Latella, Lilla Bonanno, Paulo Cezar Rocha dos Santos, Marcos Maldonado‐Díaz, Meir Plotnik, Rosaria De Luca, Angelo Quartarone, Rocco Salvatore Calabrò   +8 more
wiley   +1 more source