Results 1 to 10 of about 396,913 (336)

Colorimetric Detection of the Tuberculosis Complex Using Cycling Probe Technology and Hybridization in Microplates [PDF]

BioTechniques, 2000
Cycling probe technology (CPT) is a simple signal amplification method for the detection of specific target DNA sequences. CPT uses a chimeric DNA-RNA-DNA probe that is cut by RNase H when bound to its complementary target sequence.
S. Warnon, N. Zammatteo, I. Alexandre, C. Hans, J. Remacle   +4 more
doaj   +2 more sources

A Novel Hybridization LC-MS/MS Methodology for Quantification of siRNA in Plasma, CSF and Tissue Samples

Molecules, 2023
Therapeutic oligonucleotides, such as antisense oligonucleotide (ASO) and small interfering RNA (siRNA), are a new class of therapeutics rapidly growing in drug discovery and development.
Long Yuan, Jean-François Dupuis, Kevork Mekhssian   +2 more
doaj   +1 more source

Detection of mutations in the rpoB gene of rifampicin-resistant Mycobacterium tuberculosis strains inhibiting wild type probe hybridization in the MTBDR plus assay by DNA sequencing directly from clinical specimens

BMC Microbiology, 2020
Background The potential of genetic testing for rapid and accurate diagnosis of drug-resistant Mycobacterium tuberculosis strains is vital for efficient treatment and reduction in dissemination.
Pallavi Sinha, G. N. Srivastava, Rajneesh Tripathi, Mukti Nath Mishra, Shampa Anupurba   +4 more
doaj   +1 more source

Design and Experimental Evaluation of a New RNA-FISH Probe to Detect and Identify Paenibacillus sp.

Applied Sciences, 2022
Paenibacillus, rod-saped gram-positive endospores forming aerobic or facultative anaerobic bacteria, colonize diverse ecosystems and are involved in the biodegradation of cultural heritage assets.
Sílvia Arantes, Patrícia Branco, Ana Teresa Caldeira   +2 more
doaj   +1 more source

Low Sensitivity of Real Time PCRs Targeting Retrotransposon Sequences for the Detection of Schistosoma japonicum Complex DNA in Human Serum

Pathogens, 2021
While hybridization probe-based real-time PCR assays targeting highly repetitive multi-copy genome sequences for the diagnosis of S. mansoni complex or S.
Hagen Frickmann, Ulrike Loderstädt, Beatrice Nickel, Sven Poppert, Peter Odermatt, Somphou Sayasone, Marjan Van Esbroeck, Isabel Micalessi, Lieselotte Cnops, Poom Adisakwattana, Gérard Leboulle, Olfert Landt, Thorsten Thye, Egbert Tannich   +13 more
doaj   +1 more source

One Single Tube Reaction of Aptasensor-Based Magnetic Sensing System for Selective Fluorescent Detection of VEGF in Plasma

Biosensors, 2023
In this study, a simple, easy and convenient fluorescent sensing system for the detection of the vascular endothelial growth factor (VEGF) based on VEGF aptamers, aptamer-complementary fluorescence-labeled probe and streptavidin magnetic beads was ...
Hwang-Shang Kou, Shao-Tsung Lo, Chun-Chi Wang   +2 more
doaj   +1 more source

Hybridization thermodynamics of NimbleGen Microarrays

BMC Bioinformatics, 2010
Background While microarrays are the predominant method for gene expression profiling, probe signal variation is still an area of active research.
Posekany Alexandra, Leparc Germán G, Mueckstein Ulrike, Hofacker Ivo, Kreil David P   +4 more
doaj   +1 more source

Crustal Failure as a Tool to Probe Hybrid Stars

The Astrophysical Journal, 2023
Abstract It is currently unknown if neutron stars (NSs) are composed of nucleons only or are hybrid stars, i.e., in addition to nucleonic crusts and outer cores, they also possess quark cores. Quantum chromodynamics allows for such a possibility, but accurate calculations relevant for compact stars are still elusive.
Jonas P. Pereira, Michał Bejger, Paweł Haensel, Julian Leszek Zdunik   +3 more
openaire   +3 more sources

Mutational Scanning of PCR Products by Subtractive Oligonucleotide Hybridization Analysis

BioTechniques, 1999
Here, we describe a new approach for mutational scanning of PCR products through hybridization analysis between complementary oligonucleotides. Sets of overlapping probe oligonucleotides complementary to wild-type (WT) sequence are hybridized to ...
P. Nilsson, A. Larsson, J. Lundeberg, M. Uhlén, P.-Å. Nygren   +4 more
doaj   +1 more source

Specific and Nonspecific Hybridization of Oligonucleotide Probes on Microarrays [PDF]

Biophysical Journal, 2005
Gene expression analysis by means of microarrays is based on the sequence specific binding of mRNA to DNA oligonucleotide probes and its measurement using fluorescent labels. The binding of RNA fragments involving other sequences than the intended target is problematic because it adds a "chemical background" to the signal, which is not related to the ...
Stephan Preibisch, Hans Binder
openaire   +4 more sources