Results 1 to 10 of about 406,354 (305)

Evolution of satellite DNA sequences in two tribes of Bovidae: a cautionary tale [PDF]

Genetics and Molecular Biology, 2015
Two clones, Bt1 from Bos taurus and Om1 from Ovis orientalis musimon, were used as probes for hybridization on genomic DNA and on metaphase chromosomes in members of Bovini and Caprini tribes. Bt1 and Om1 are sequences respectively belonging to the 1.715
Coni, Pp, Dedola, Gl, Dettori, Ml, MEZZANOTTE, ROBERTO, NIEDDU, MARIELLA, Pazzola, M, PICHIRI, GIUSEPPINA, ROBLEDO, RENATO, Vacca, Gm   +8 more
core   +3 more sources

In vitro identification and in silico utilization of interspecies sequence similarities using GeneChip(® )technology [PDF]

BMC Genomics, 2005
BACKGROUND: Genomic approaches in large animal models (canine, ovine etc) are challenging due to insufficient genomic information for these species and the lack of availability of corresponding microarray platforms. To address this problem, we speculated
Garcia, Joe GN, Grigoryev, Dmitry N, Irizarry, Rafael A, Ma, Shwu-Fan, Simon, Brett A, Ye, Shui Q   +5 more
core   +5 more sources

Enhanced Hybridization Selectivity Using Structured GammaPNA Probes [PDF]

Molecules, 2020
High affinity nucleic acid analogues such as gammaPNA (γPNA) are capable of invading stable secondary and tertiary structures in DNA and RNA targets but are susceptible to off-target binding to mismatch-containing sequences.
Taylor D. Canady, April S. Berlyoung, Joe A. Martinez, Cole Emanuelson, Cheryl A. Telmer, Marcel P. Bruchez, Bruce A. Armitage   +6 more
doaj   +3 more sources

Protocol for multiplex whole-mount RNA fluorescence in situ hybridization combined with immunohistochemistry in the mosquito brain. [PDF]

STAR Protoc
Summary: Advances in sequencing technologies have enabled transcriptional profiling of previously understudied yet critical species, such as mosquitoes. Here, we present a protocol for multiplex whole-mount RNA fluorescence in situ hybridization combined
Suppermpool A, Trivedi C, Powell GT, Andrés M.   +3 more
europepmc   +2 more sources

Protocol to detect RNAs from tissue sections in mice using Y-branched probe in situ hybridization

STAR Protocols, 2022
Summary: Here, we describe a fluorescent in situ hybridization protocol named Yn-situ, standing for Y-branched probe in situ hybridization, to detect RNAs from mice tissue sections.
Yunming Wu, C. Ron Yu
doaj   +1 more source

A Novel Hybridization LC-MS/MS Methodology for Quantification of siRNA in Plasma, CSF and Tissue Samples

Molecules, 2023
Therapeutic oligonucleotides, such as antisense oligonucleotide (ASO) and small interfering RNA (siRNA), are a new class of therapeutics rapidly growing in drug discovery and development.
Long Yuan, Jean-François Dupuis, Kevork Mekhssian   +2 more
doaj   +1 more source

Detection of mutations in the rpoB gene of rifampicin-resistant Mycobacterium tuberculosis strains inhibiting wild type probe hybridization in the MTBDR plus assay by DNA sequencing directly from clinical specimens

BMC Microbiology, 2020
Background The potential of genetic testing for rapid and accurate diagnosis of drug-resistant Mycobacterium tuberculosis strains is vital for efficient treatment and reduction in dissemination.
Pallavi Sinha, G. N. Srivastava, Rajneesh Tripathi, Mukti Nath Mishra, Shampa Anupurba   +4 more
doaj   +1 more source

One Single Tube Reaction of Aptasensor-Based Magnetic Sensing System for Selective Fluorescent Detection of VEGF in Plasma

Biosensors, 2023
In this study, a simple, easy and convenient fluorescent sensing system for the detection of the vascular endothelial growth factor (VEGF) based on VEGF aptamers, aptamer-complementary fluorescence-labeled probe and streptavidin magnetic beads was ...
Hwang-Shang Kou, Shao-Tsung Lo, Chun-Chi Wang   +2 more
doaj   +1 more source

Low Sensitivity of Real Time PCRs Targeting Retrotransposon Sequences for the Detection of Schistosoma japonicum Complex DNA in Human Serum

Pathogens, 2021
While hybridization probe-based real-time PCR assays targeting highly repetitive multi-copy genome sequences for the diagnosis of S. mansoni complex or S.
Hagen Frickmann, Ulrike Loderstädt, Beatrice Nickel, Sven Poppert, Peter Odermatt, Somphou Sayasone, Marjan Van Esbroeck, Isabel Micalessi, Lieselotte Cnops, Poom Adisakwattana, Gérard Leboulle, Olfert Landt, Thorsten Thye, Egbert Tannich   +13 more
doaj   +1 more source

Design and Experimental Evaluation of a New RNA-FISH Probe to Detect and Identify Paenibacillus sp.

Applied Sciences, 2022
Paenibacillus, rod-saped gram-positive endospores forming aerobic or facultative anaerobic bacteria, colonize diverse ecosystems and are involved in the biodegradation of cultural heritage assets.
Sílvia Arantes, Patrícia Branco, Ana Teresa Caldeira   +2 more
doaj   +1 more source