Results 11 to 20 of about 396,913 (336)

Evolution of satellite DNA sequences in two tribes of Bovidae: A cautionary tale [PDF]

open access: yesGenetics and Molecular Biology, 2015
Two clones, Bt1 from Bos taurus and Om1 from Ovis orientalis musimon, were used as probes for hybridization on genomic DNA and on metaphase chromosomes in members of Bovini and Caprini tribes. Bt1 and Om1 are sequences respectively belonging to the 1.715
Mariella Nieddu   +8 more
doaj   +2 more sources

In vitro identification and in silico utilization of interspecies sequence similarities using GeneChip® technology [PDF]

open access: yesBMC Genomics, 2005
Background Genomic approaches in large animal models (canine, ovine etc) are challenging due to insufficient genomic information for these species and the lack of availability of corresponding microarray platforms.
Ye Shui Q   +5 more
doaj   +4 more sources

Compressive Sensing DNA Microarrays [PDF]

open access: yes, 2008
Compressive sensing microarrays (CSMs) are DNA-based sensors that operate using group testing and compressive sensing (CS) principles. In contrast to conventional DNA microarrays, in which each genetic sensor is designed to respond to a single target, in
Baraniuk, RG   +3 more
core   +1 more source

Rapid identification and differentiation of the vaccine strain Rac H from EHV 1 field isolates using a non-radioactive DNA probe [PDF]

open access: yes, 1992
A method for rapid differentiation between the EHV 1 live vaccine strain Rac H and field isolates is described. Total DNA was isolated from virus-infected small scale cell cultures.
Eichhorn, Werner   +3 more
core   +1 more source

Morphological detection of X- and Y-chromosomes in smears and paraffin-embedded tissues using a non-isotopic in situ hybridization technique (NISH) [PDF]

open access: yes, 1994
Pharyngeal smears and paraffin-embedded tissue specimens (skeletal muscle, kidney) obtained from 10 male and 10 female individuals were evaluated using non-isotopic in situ hybridization (NISH) with commercial X- and Y-specific biotinylated probes which ...
Baretton, G.   +4 more
core   +1 more source

γPNA FRET Pair Miniprobes for Quantitative Fluorescent In Situ Hybridization to Telomeric DNA in Cells and Tissue

open access: yesMolecules, 2017
Measurement of telomere length by fluorescent in situ hybridization is widely used for biomedical and epidemiological research, but there has been relatively little development of the technology in the 20 years since it was first reported.
Alexander Orenstein   +10 more
doaj   +1 more source

A model of binding on DNA microarrays: understanding the combined effect of probe synthesis failure, cross-hybridization, DNA fragmentation and other experimental details of affymetrix arrays

open access: yesBMC Genomics, 2012
Background DNA microarrays are used both for research and for diagnostics. In research, Affymetrix arrays are commonly used for genome wide association studies, resequencing, and for gene expression analysis.
Jakubek Yasminka A, Cutler David J
doaj   +1 more source

Hybridization of RNA Probes v1

open access: yes, 2020
Over the past decade a plethora of noncoding RNAs (ncRNAs) have been identified, initiating an explosion in RNA research. Although RNA sequencing methods provide unsurpassed insights into ncRNA distribution and expression, detailed information on structure and processing are harder to extract from sequence data.
Jonathan Houseley, Cristina Cruz
openaire   +4 more sources

Protocol to detect RNAs from tissue sections in mice using Y-branched probe in situ hybridization

open access: yesSTAR Protocols, 2022
Summary: Here, we describe a fluorescent in situ hybridization protocol named Yn-situ, standing for Y-branched probe in situ hybridization, to detect RNAs from mice tissue sections.
Yunming Wu, C. Ron Yu
doaj  

Continuously tunable nucleic acid hybridization probes [PDF]

open access: yesNature Methods, 2015
In silico-designed nucleic acid probes and primers often do not achieve favorable specificity and sensitivity tradeoffs on the first try, and iterative empirical sequence-based optimization is needed, particularly in multiplexed assays. We present a novel, on-the-fly method of tuning probe affinity and selectivity by adjusting the stoichiometry of ...
Lucia R Wu   +10 more
openaire   +4 more sources

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