Results 11 to 20 of about 406,354 (305)

Continuously tunable nucleic acid hybridization probes [PDF]

Nature Methods, 2015
In silico-designed nucleic acid probes and primers often do not achieve favorable specificity and sensitivity tradeoffs on the first try, and iterative empirical sequence-based optimization is needed, particularly in multiplexed assays. We present a novel, on-the-fly method of tuning probe affinity and selectivity by adjusting the stoichiometry of ...
Lucia R Wu, Juexiao Sherry Wang, John Z Fang, Emily R Evans, Alessandro Pinto, Irena Pekker, Richard Boykin, Celine Ngouenet, Philippa J Webster, Joseph Beechem, David Yu Zhang   +10 more
openaire   +3 more sources

Physico-chemical foundations underpinning microarray and next-generation sequencing experiments [PDF]

, 2013
Hybridization of nucleic acids on solid surfaces is a key process involved in high-throughput technologies such as microarrays and, in some cases, next-generation sequencing (NGS).
A. Buhot, A. E. Pozhitkov, A. Halperin, A. Harrison, A. Ott, Amend, B. M. Pettitt, Berger, Binder, Binder, Binder, Binder, Bolstad, Bullard, Burden, Burden, C. Gibas, C. J. Burden, Chou, Chou, Czypionka, D. P. Kreil, D. Tautz, E. Carlon, Fasold, Fasold, Fiche, Fuchs, H. Binder, Halperin, Harr, Harrison, He, Heim, Held, Hooyberghs, Huettel, Iltumur, Irizarry, Irizarry, Irizarry, Irving, J. Hooyberghs, Kane, L. J. Gamble, Lee, Lee, Letowski, Li, Liebich, Lockhart, Luebke, Marshall, Matveeva, Mueckstein, Mulders, Naiser, Naiser, P. A. Noble, Pingel, Pozhitkov, R. Levicky, Relogio, Rouillard, Tanaka, Trapp, Upton, Vainrub, Wodicka, Yu, Zhang   +70 more
core   +8 more sources

Compressive Sensing DNA Microarrays [PDF]

, 2008
Compressive sensing microarrays (CSMs) are DNA-based sensors that operate using group testing and compressive sensing (CS) principles. In contrast to conventional DNA microarrays, in which each genetic sensor is designed to respond to a single target, in
Baraniuk, RG, Dai, W, Milenkovic, O, Sheikh, MA   +3 more
core   +1 more source

Rapid identification and differentiation of the vaccine strain Rac H from EHV 1 field isolates using a non-radioactive DNA probe [PDF]

, 1992
A method for rapid differentiation between the EHV 1 live vaccine strain Rac H and field isolates is described. Total DNA was isolated from virus-infected small scale cell cultures.
Eichhorn, Werner, Hübert, P. H., Meyer, H., Schwend, C.   +3 more
core   +1 more source

Mutational Scanning of PCR Products by Subtractive Oligonucleotide Hybridization Analysis

BioTechniques, 1999
Here, we describe a new approach for mutational scanning of PCR products through hybridization analysis between complementary oligonucleotides. Sets of overlapping probe oligonucleotides complementary to wild-type (WT) sequence are hybridized to ...
P. Nilsson, A. Larsson, J. Lundeberg, M. Uhlén, P.-Å. Nygren   +4 more
doaj   +1 more source

Morphological detection of X- and Y-chromosomes in smears and paraffin-embedded tissues using a non-isotopic in situ hybridization technique (NISH) [PDF]

, 1994
Pharyngeal smears and paraffin-embedded tissue specimens (skeletal muscle, kidney) obtained from 10 male and 10 female individuals were evaluated using non-isotopic in situ hybridization (NISH) with commercial X- and Y-specific biotinylated probes which ...
Baretton, G., Betz, P., Eisenmenger, Wolfgang, Nerlich, A., Tübel, J.   +4 more
core   +1 more source

Hybridization thermodynamics of NimbleGen Microarrays

BMC Bioinformatics, 2010
Background While microarrays are the predominant method for gene expression profiling, probe signal variation is still an area of active research.
Posekany Alexandra, Leparc Germán G, Mueckstein Ulrike, Hofacker Ivo, Kreil David P   +4 more
doaj   +1 more source

Rapid metaphase and interphase detection of radiation-induced chromosome aberrations in human lymphocytes by chromosomal suppression in situ hybridization [PDF]

, 1990
Chromosomal in situ suppression (CISS)-hybridization of biotinylated phage DNA-library inserts from sorted human chromosomes was used to decorate chromosomes 1 and 7 specifically from pter to qter and to detect structural aberrations of these chromosomes
Cremer, Christoph, Cremer, Thomas, Emmerich, Patricia, Lichter, Peter, Popp, Susanne   +4 more
core   +1 more source

γPNA FRET Pair Miniprobes for Quantitative Fluorescent In Situ Hybridization to Telomeric DNA in Cells and Tissue

Molecules, 2017
Measurement of telomere length by fluorescent in situ hybridization is widely used for biomedical and epidemiological research, but there has been relatively little development of the technology in the 20 years since it was first reported.
Alexander Orenstein, April S. Berlyoung, Elizabeth E. Rastede, Ha H. Pham, Elise Fouquerel, Connor T. Murphy, Brian J. Leibowitz, Jian Yu, Tumul Srivastava, Bruce A. Armitage, Patricia L. Opresko   +10 more
doaj   +1 more source

A model of binding on DNA microarrays: understanding the combined effect of probe synthesis failure, cross-hybridization, DNA fragmentation and other experimental details of affymetrix arrays

BMC Genomics, 2012
Background DNA microarrays are used both for research and for diagnostics. In research, Affymetrix arrays are commonly used for genome wide association studies, resequencing, and for gene expression analysis.
Jakubek Yasminka A, Cutler David J
doaj   +1 more source