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Probe-Based (Hybridization) Methods
2000The conceptual basis of the probe is outlined on pages 19/20. Probes have a variety of uses, which include: Detection/identification of pathogens Detection of specific (e.g. toxin) genes Localization of an (intracellular) target sequence (in situ hybridization, ISH) Confirmation of correct sequence in the products from nucleic acid
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Molecular Beacons: Probes that Fluoresce upon Hybridization
Nature Biotechnology, 1996We have developed novel nucleic acid probes that recognize and report the presence of specific nucleic acids in homogeneous solutions. These probes undergo a spontaneous fluorogenic conformational change when they hybridize to their targets. Only perfectly complementary targets elicit this response, as hybridization does not occur when the target ...
S, Tyagi, F R, Kramer
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Photobiotin-Labeled DNA and RNA Hybridization Probes
2003Nucleic acid hybridization, the formation of a duplex between two complementary nucleotide sequences, is being increasingly utilized in the research laboratory and for the routine diagnosis of disease. Biotin-labeled nucleic acid hybridization probes have advantages over radioactively labeled probes in terms of stability, safety, and time of detection.
J L, McInnes, A C, Forster, R H, Symons
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Production and Use of Nonradioactive Hybridization Probes
2003Molecular hybridization is a useful technique for identifying specific target sequences even when they are present as a single copy in a complex population. It can be performed either on a solid matrix on which pure DNA (or RNA) is bound (blot hybridization) or on tissue sections (in situ hybridization). Until recently, the probes used in hybridization
V T, Chan, J O, McGee
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Preparation of Probes for Hybridization
2000Preparation of probes for hybridization involves in vitro incorporation of reporter molecules into nuclei acids. These reporters can be incorporated at one or both ends of nucleic acid molecules, giving specific, low density labeled probes. High density labeling is usually achieved by incorporating the reporters uniformly throughout entire length of ...
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Operando Scanning Electrochemical Probe Microscopy during Electrocatalysis
Chemical Reviews, 2023, Wolfgang Schuhmann, Corina Andronescu
exaly
In Situ Hybridization for RNA: Nonradioactive Probe: RNA Probe
2000Non-radioactive in situ hybridization method is now widely used to detect nucleic acid in tissue sections. However, the results for the detection of tissue mRNA are not always consistent. We have to consider several factors for this purpose. We describe our current methods for the most consistent detection.
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