Results 31 to 40 of about 406,354 (305)

Electrically Guided DNA Immobilization and Multiplexed DNA Detection with Nanoporous Gold Electrodes. [PDF]

, 2018
Molecular diagnostics have significantly advanced the early detection of diseases, where the electrochemical sensing of biomarkers (e.g., DNA, RNA, proteins) using multiple electrode arrays (MEAs) has shown considerable promise.
Daggumati, Pallavi, Li, Zidong, Seker, Erkin, Veselinovic, Jovana   +3 more
core   +3 more sources

Modification of comet-FISH technique by using temperature instead of chemical denaturation

MethodsX, 2014
Comet-FISH technique is an extension of commonly used comet assay. Its purpose is to determine whether primary DNA damage which comet assay detects occurred within a sequence of interest that is visualized by hybridization of fluorescent probe.
Marin Mladinic, Davor Zeljezic
doaj   +1 more source

Development and comparison of cross-linking and non-crosslinking probe-gold nanoparticle hybridization assays for direct detection of unamplified bovine viral diarrhea virus-RNA

BMC Biotechnology, 2021
Background Bovine viral diarrhea virus (BVDV) is a major economic disease that has been spread in most countries. In addition to vaccination, one of the main ways to control the disease and prevent it from spreading is to detect and cull infected animals,
Zahra Heidari, Seyedeh Elham Rezatofighi, Saadat Rastegarzadeh   +2 more
doaj   +1 more source

Genosensing on a 3D-printed nanocarbon electrode

Electrochemistry Communications, 2023
In this paper we present the characterization of 3D-printed nanocarbon electrodes (3DnCes) and their application in electrochemical enzyme-linked detection of DNA hybridization.
Jyoti, Miroslav Fojta, Monika Hermanová, Hana Pivoňková, Osamah Alduhaish, Martin Pumera   +5 more
doaj   +1 more source

Physics-based analysis of Affymetrix microarray data

, 2006
We analyze publicly available data on Affymetrix microarrays spike-in experiments on the human HGU133 chipset in which sequences are added in solution at known concentrations. The spike-in set contains sequences of bacterial, human and artificial origin.
Barkema, G. T., Carlon, E., Heim, T., Tranchevent, L. -C.   +3 more
core   +1 more source

Characterization of two marker chromosomes in a patient with acute nonlymphocytic leukemia by two-color fluorescence in situ hybridization [PDF]

, 1993
A patient with acute nonlymphocytic leukemia (ANLL), M5b according to French-American-British (FAB) classification, showed monosomy 16, an extra 1p−, and a 21q+. These derivative chromosomes could not be defined by GTG-banding.
Anna Jauch, Brothman, Burke, Christoph Lengauer, Collins, Cooke, Cremer, Cremer, Jauch, Klever, Lengauer, Lengauer, Lengauer, Lichter, Lichter, Marosi, Masafumi Taniwaki, Michael R. Speicher, Moyzis, Pinkel, Pinkel, Rowley, Selleri, Smit, Speleman, Susanne Popp, Thomas Cremer   +26 more
core   +1 more source

Interplay between circadian and other transcription factors—Implications for cycling transcriptome reprogramming

FEBS Letters, EarlyView.
This perspective highlights emerging insights into how the circadian transcription factor CLOCK:BMAL1 regulates chromatin architecture, cooperates with other transcription factors, and coordinates enhancer dynamics. We propose an updated framework for how circadian transcription factors operate within dynamic and multifactorial chromatin landscapes ...
Xinyu Y. Nie, Jerome S. Menet
wiley   +1 more source

The role of histone modifications in transcription regulation upon DNA damage

FEBS Letters, EarlyView.
This review discusses the critical role of histone modifications in regulating gene expression during the DNA damage response (DDR). By modulating chromatin structure and recruiting repair factors, these post‐translational modifications fine‐tune transcriptional programmes to maintain genomic stability.
Angelina Job Kolady, Siyao Wang
wiley   +1 more source

Detection of monosomy 7 in interphase cells of patients with myeloid disorders [PDF]

, 1990
Six patients, five with acute myeloid leukemia (AML) and one with a myelodysplastic syndrome (MDS), were found to have monosomy 7 by conventional cytogenetics at diagnosis.
Cremer, Thomas, Gezer, Sefer, Kolluri, Rukmini V., Manuelidis, L., Raza, Azra, Sait, Sheila   +5 more
core   +1 more source

Comparative evaluation for the globin gene depletion methods for mRNA sequencing using the whole blood-derived total RNAs

BMC Genomics, 2020
Background There are challenges in generating mRNA-Seq data from whole-blood derived RNA as globin gene and rRNA are frequent contaminants. Given the abundance of erythrocytes in whole blood, globin genes comprise some 80% or more of the total RNA ...
Jin Sung Jang, Brianna Berg, Eileen Holicky, Bruce Eckloff, Mark Mutawe, Minerva M. Carrasquillo, Nilüfer Ertekin-Taner, Julie M. Cuninngham   +7 more
doaj   +1 more source