Results 31 to 40 of about 396,913 (336)
A novel cassette method for probe evaluation in the designed biochips [PDF]
, 2014 A critical step in biochip design is the selection of probes with identical hybridisation characteristics. In this article we describe a novel method for evaluating DNA hybridisation probes, allowing the fine-tuning of biochips, that uses cassettes with ...
Al-humam, Abdulmohsen A. +7 more
core +9 more sources
Biosensors, 2012 Two quartz crystal microbalance oligonucleotide biosensors of a toxic microalga gene sequence (Alexandrium Minutum) have been designed. Grafting on a gold surface of 20-base thiol- or biotin-labeled probe, and selective hybridization with the ...
Mathieu Lazerges +3 more
doaj +1 more source
The Sensitivity of Sag 1 and Bag 1 Probes to Detect Toxoplasma Gondii in the Free-Rearing Chicken [PDF]
, 2012 This study was aimed to determine the sensitivity of Sag1 and Bag1 Probe to detect in free-rearing chicken using dot blot hybridization method. Thirty serologically free-rearing chicken toxoplasmosis DNA were used as samples in this study.
Apsari, I. A. (Ida)
core +2 more sources
Modification of comet-FISH technique by using temperature instead of chemical denaturation
MethodsX, 2014 Comet-FISH technique is an extension of commonly used comet assay. Its purpose is to determine whether primary DNA damage which comet assay detects occurred within a sequence of interest that is visualized by hybridization of fluorescent probe.
Marin Mladinic, Davor Zeljezic
doaj +1 more source
BMC Biotechnology, 2021 Background Bovine viral diarrhea virus (BVDV) is a major economic disease that has been spread in most countries. In addition to vaccination, one of the main ways to control the disease and prevent it from spreading is to detect and cull infected animals,
Zahra Heidari +2 more
doaj +1 more source
Painting of human chromosomes with probes generated from hybrid cell lines by PCR with Alu and L1 primers [PDF]
, 1990 Specific amplification of human sequences of up to several kb length has recently been accomplished in man-hamster and man-mouse somatic hybrid cell DNA by IRS-PCR (interspersed repetitive sequence — polymerase chain reaction).
Cremer, Thomas +2 more
core +1 more source
DNA hybridization probe for the Pseudomonas fluorescens group [PDF]
Applied and Environmental Microbiology, 1986 Plasmid pHF360 was constructed from cloned rRNA genes (rDNA) of Pseudomonas aeruginosa and used as hybridization probe for the Pseudomonas fluorescens group. The probe was tested by dot and in situ colony hybridizations to chromosomal DNAs from a wide variety of organisms. pHF360 DNA hybridized exclusively to chromosomal DNAs from bacteria representing
Wolfgang Ludwig +2 more
openaire +3 more sources
Fluorescence In Situ Hybridization Probe Preparation
, 2016 The public human genome sequencing project utilized a hierarchical approach. A large number of BAC/PAC clones, with an insert size approximate from 50 kb to 300 kb, were identified and finely mapped with respect to the Sequence Tagged Site (STS) physical map and with respect to each other.
TOLOMEO, DORON +2 more
openaire +4 more sources
The effects of mismatches on hybridization in DNA microarrays: determination of nearest neighbor parameters [PDF]
, 2010 Quantifying interactions in DNA microarrays is of central importance for a better understanding of their functioning. Hybridization thermodynamics for nucleic acid strands in aqueous solution can be described by the so-called nearest-neighbor model ...
Bloomfield +23 more
core +3 more sources
BMC Genomics, 2020 Background There are challenges in generating mRNA-Seq data from whole-blood derived RNA as globin gene and rRNA are frequent contaminants. Given the abundance of erythrocytes in whole blood, globin genes comprise some 80% or more of the total RNA ...
Jin Sung Jang +7 more
doaj +1 more source