Development of real-time PCR and hybridization methods for detection and identification of thermophilic Campylobacter spp. in pig faecal samples [PDF]
, 2005 Aims: To develop a real-time (rt) PCR for species differentiation of thermophilic Campylobacter and to develop a method for assessing co-colonization of pigs by Campylobacter spp. Methods and results: The specificity of a developed 5’nuclease rt-PCR for
Al Rashid S.T. +10 more
core +1 more source
The biophysics of DNA hybridization with immobilized oligonucleotide probes
Biophysical Journal, 1995 A mathematical model based on receptor-ligand interactions at a cell surface has been modified and further developed to represent heterogeneous DNA-DNA hybridization on a solid surface. The immobilized DNA molecules with known sequences are called probes, and the DNA molecules in solution with unknown sequences are called targets in this model. Capture
Steven E. McKenzie +2 more
openaire +3 more sources
Network topology drives population temporal variability in experimental habitat networks
Population Ecology, EarlyView.Habitat patches connected by dispersal pathways form habitat networks. We explored how network topology affects population outcomes in laboratory experiments using a model species (Daphnia carinata). Central habitat nodes in complex lattice networks exhibited lower temporal variability in population sizes, suggesting they support more stable ...
Yiwen Xu +3 more
wiley +1 more source
Thermodynamics of RNA/DNA hybridization in high density oligonucleotide microarrays
, 2006 We analyze a series of publicly available controlled experiments (Latin square) on Affymetrix high density oligonucleotide microarrays using a simple physical model of the hybridization process.
Bolstad +16 more
core +1 more source
Zip Nucleic Acid-Based Genomagnetic Assay for Electrochemical Detection of microRNA-34a
Biosensors, 2023 Zip nucleic acid (ZNA)-based genomagnetic assay was developed herein for the electrochemical detection of microRNA-34a (miR-34a), which is related to neurological disorders and cancer.
Arzum Erdem, Ece Eksin
doaj +1 more source
FEBS Letters, EarlyView.Interaction extracellular vesicles (iEVs) are hybrid vesicles formed through host‐pathogen communication. They facilitate immune evasion, transfer pathogens' molecules, increase host cell uptake, and enhance virulence. This Perspective article illustrates the multifunctional roles of iEVs and highlights their emerging relevance in infection dynamics ...
Bruna Sabatke +2 more
wiley +1 more source
Low density DNA microarray for detection of most frequent
BMC Biotechnology, 2005 Background We have developed an oligonucleotide microarray (genosensor) utilizing a double tandem hybridization technique to search for 9 point mutations located in the most frequently altered codons of the TP53 gene.
Espinosa-Lara Juana +5 more
doaj +1 more source
A stepwise emergence of evolution in the RNA world
FEBS Letters, EarlyView.How did biological evolution emerge from chemical reactions? This perspective proposes a gradual scenario of self‐organization among RNA molecules, where catalytic feedback on random mixtures plays the central role. Short oligomers cross‐ligate, and self‐assembly enables heritable variations. An event of template‐externalization marks the transition to
Philippe Nghe
wiley +1 more source
Effect of unlabeled helper probes on detection of an RNA target by bead-based sandwich hybridization
BioTechniques, 2004 Unlabeled helper oligonucleotides assisting a bead-based sandwich hybridization assay were tested for the optimal placement of the capture and detection probes. The target used was a full-length in vitro synthesized mRNA molecule.
Kim Bundvig Barken +3 more
doaj +1 more source
Physico-chemical foundations underpinning microarray and next-generation sequencing experiments [PDF]
, 2013 Hybridization of nucleic acids on solid surfaces is a key process involved in high-throughput technologies such as microarrays and, in some cases, next-generation sequencing (NGS).
A. Buhot +70 more
core +3 more sources