Results 51 to 60 of about 406,354 (305)

Effect of unlabeled helper probes on detection of an RNA target by bead-based sandwich hybridization

BioTechniques, 2004
Unlabeled helper oligonucleotides assisting a bead-based sandwich hybridization assay were tested for the optimal placement of the capture and detection probes. The target used was a full-length in vitro synthesized mRNA molecule.
Kim Bundvig Barken, Magdalena Gabig-Ciminska, Anders Holmgren, Søren Molin   +3 more
doaj   +1 more source

Probe‐compensated fast hybrid antenna testing

IET Microwaves, Antennas & Propagation, 2017
Near‐field (NF) measurements with NF‐to‐far‐field (FF) transformation are a powerful and standard approach to modern antenna measurements. This approach implies a relatively close distance between device under test (DUT) and probe and, since the latter is rarely an ideal probe, it will introduce effects that have to be removed to obtain a FF which is ...
Giordanengo, Giorgio, Righero, Marco, Araque Quijano, Javier Leonardo, Vipiana, Francesca, Vecchi, Giuseppe   +4 more
openaire   +2 more sources

Modeling hepatic fibrosis in TP53 knockout iPSC‐derived human liver organoids

Molecular Oncology, EarlyView.
This study developed iPSC‐derived human liver organoids with TP53 gene knockout to model human liver fibrosis. These organoids showed elevated myofibroblast activation, early disease markers, and advanced fibrotic hallmarks. The use of profibrotic differentiation medium further amplified the fibrotic signature seen in the organoids.
Mustafa Karabicici, Soheil Akbari, Ceyda Caliskan, Canan Celiker, Ozden Oz, Leman Binokay, Gökhan Karakulah, Serif Senturk, Esra Erdal   +8 more
wiley   +1 more source

Cytological and molecular characterization of wheat lines with Thinopyrum intermedium chromosome additions, substitutions and translocations resistant to barley yellow dwarf virus [PDF]

, 1999
Barley yellow dwarf virus (BYDV) is the most serious viral disease affecting wheat and genes for BYDV resistance have not been found in wheat. BYDV-resistant alien addition and alien substitution lines produced from a wheat × Thinopyrum intermedium ...
Crasta, O., Francki, M., Gyulai, Gábor, Sharma, H.   +3 more
core   +1 more source

Fluorescent hybridization probes for nucleic acid detection [PDF]

Analytical and Bioanalytical Chemistry, 2011
Due to their high sensitivity and selectivity, minimum interference with living biological systems, and ease of design and synthesis, fluorescent hybridization probes have been widely used to detect nucleic acids both in vivo and in vitro. Molecular beacons (MBs) and binary probes (BPs) are two very important hybridization probes that are designed ...
Jia, Guo, Jingyue, Ju, Nicholas J, Turro
openaire   +2 more sources

Tumor and germline testing with next generation sequencing in epithelial ovarian cancer: a prospective paired comparison using an 18‐gene panel

Molecular Oncology, EarlyView.
Genetic testing in epithelial ovarian cancer includes both germline and tumor‐testing. This approach often duplicates resources. The current prospective study assessed the feasibility of tumor‐first multigene testing by comparing tumor tissue with germline testing of peripheral blood using an 18‐gene NGS panel in 106 patients.
Elisabeth Spenard, Cristina Mitric, Melanie Care, Tracy L. Stockley, Raymond H. Kim, Jeanna McCuaig, Blaise Clarke, Laura Ranich, Clare Sheen, Sarah E. Ferguson, Liat Hogen, Taymaa May, Marcus Q. Bernardini   +12 more
wiley   +1 more source

Chromosomal Localization of the Carcinoembryonic Antigen Gene Family and Differential Expression in Various Tumors [PDF]

, 1988
Carcinoembryonic antigen (CEA) is a glycoprotein which is important as a tumor marker for a number of human cancers. It is a member of a gene family comprising about 10 closely related genes.
Fiebig, Heinz-Herbert, Kleist, Sabine von, Ortlieb, Barbara, Rudert, Fritz, Schempp, Werner, Shively, John E., Thompson, John A., Weber, Bernhard, Zimmermann, Wolfgang   +8 more
core   +1 more source

Thermodynamic behavior of short oligonucleotides in microarray hybridizations can be described using Gibbs free energy in a nearest-neighbor model

, 2007
While designing oligonucleotide-based microarrays, cross-hybridization between surface-bound oligos and non-intended labeled targets is probably the most difficult parameter to predict. Although literature describes rules-of-thumb concerning oligo length,
Carlon, E., De Vuyst, L., Van Hummelen, P., Weckx, S.   +3 more
core   +1 more source

Investigation of marmoset hybrids (Cebuella pygmaea x Callithrix jacchus) and related Callitrichinae (Platyrrhini) by cross-species chromosome painting and comparative genomic hybridization [PDF]

, 2005
We report on the cytogenetics of twin offspring from an interspecies cross in marmosets (Callitrichinae, Platyrrhini), resulting from a pairing between a female Common marmoset (Callithrix jacchus, 2n = 46) and a male Pygmy marmoset (Cebuella pygmaea, 2n
Canavez FC, Dugoujon JM, G. Anzenberger, M. Münch, M. Neusser, Moore CM, Muller S, Muller S, Natori M, Pellicciari C, Ratomponirina C, Rosenberger AL, Rumpler Y, Rylands AB, S. Müller, Yunis E   +15 more
core   +1 more source

Modeling formamide denaturation of probe-target hybrids for improved microarray probe design in microbial diagnostics.

PLoS ONE, 2012
Application of high-density microarrays to the diagnostic analysis of microbial communities is challenged by the optimization of oligonucleotide probe sensitivity and specificity, as it is generally unfeasible to experimentally test thousands of probes ...
L Safak Yilmaz, Alexander Loy, Erik S Wright, Michael Wagner, Daniel R Noguera   +4 more
doaj   +1 more source