Results 61 to 70 of about 396,913 (336)

UPS 2.0: unique probe selector for probe design and oligonucleotide microarrays at the pangenomic/ genomic level

BMC Genomics, 2010
Background Nucleic acid hybridization is an extensively adopted principle in biomedical research, in which the performance of any hybridization-based method depends on the specificity of probes to their targets.
Hsiung Chao A, Kuo Bao-Han, Su Sheng-Yao, Lo Chen-Zen, Chen Shu-Hwa, Lin Chung-Yen   +5 more
doaj   +1 more source

Normalization and centering of array-based heterologous genome hybridization based on divergent control probes

BMC Bioinformatics, 2011
Background Hybridization of heterologous (non-specific) nucleic acids onto arrays designed for model-organisms has been proposed as a viable genomic resource for estimating sequence variation and gene expression in non-model organisms.
Wheeler David, Jones Kenneth L, Darby Brian J, Herman Michael A   +3 more
doaj   +1 more source

Response to neoadjuvant chemotherapy in early breast cancers is associated with epithelial–mesenchymal transition and tumor‐infiltrating lymphocytes

Molecular Oncology, EarlyView.
Epithelial–mesenchymal transition (EMT) and tumor‐infiltrating lymphocytes (TILs) are associated with early breast cancer response to neoadjuvant chemotherapy (NAC). This study evaluated EMT and TIL shifts, with immunofluorescence and RNA sequencing, at diagnosis and in residual tumors as potential biomarkers associated with treatment response.
Françoise Derouane, Jérôme Ambroise, Cédric van Marcke, Mieke Van Bockstal, Martine Berlière, Christine Galant, Hélène Dano, Médina Lougué, Elena Benidovskaya, Guy Jerusalem, Vincent Bours, Claire Josse, Jérôme Thiry, Aurélie Daumerie, Caroline Bouzin, Cyril Corbet, François P. Duhoux   +16 more
wiley   +1 more source

Probe signal correction for differential methylation hybridization experiments

BMC Bioinformatics, 2008
Background Non-biological signal (or noise) has been the bane of microarray analysis. Hybridization effects related to probe-sequence composition and DNA dye-probe interactions have been observed in differential methylation hybridization (DMH) microarray
Yan Pearlly, Potter Dustin P, Huang Tim HM, Lin Shili   +3 more
doaj   +1 more source

Detection of amplified DNA sequences by reverse chromosome painting using genomic tumor DNA as probe [PDF]

, 1993
A modification of reverse chromosome painting was carried out using genomic DNA from tumor cells as a complex probe for chromosomal in situ suppression hybridization to normal metaphase chromsome spreads.
Cremer, Thomas, Joos, Stefan, Lichter, Peter, Scherthan, Harry, Schlegel, Jürgen, Speicher, Michael R.   +5 more
core   +1 more source

Peripheral blood proteome biomarkers distinguish immunosuppressive features of cancer progression

Molecular Oncology, EarlyView.
Immune status significantly influences cancer progression. This study used plasma proteomics to analyze benign 67NR and malignant 4T1 breast tumor models at early and late tumor stages. Immune‐related proteins–osteopontin (Spp1), lactotransferrin (Ltf), calreticulin (Calr) and peroxiredoxin 2 (Prdx2)–were associated with systemic myeloid‐derived ...
Yeon Ji Park, Jae Won Oh, Hyewon Chung, Jung Won Kwon, Yi Rang Na, Kwang Pyo Kim, Seung Hyeok Seok   +6 more
wiley   +1 more source

32P Radioactive Probe Synthesis and Preparation

Bio-Protocol, 2011
To probe for a specific mRNA species by Northern blot, RNA from the agarose/formaldehyde gel needs to be transferred to a nylon membrane. RNA is detected by hybridization using a labeled probe.
Bio-protocol Editorial Office
doaj   +1 more source

Modeling and Estimation for Real-Time Microarrays [PDF]

, 2008
Microarrays are used for collecting information about a large number of different genomic particles simultaneously. Conventional fluorescent-based microarrays acquire data after the hybridization phase.
Hassibi, Arjang, Hassibi, Babak, Vikalo, Haris   +2 more
core   +2 more sources

Targeting the MDM2‐MDM4 interaction interface reveals an otherwise therapeutically active wild‐type p53 in colorectal cancer

Molecular Oncology, EarlyView.
This study investigates an alternative approach to reactivating the oncosuppressor p53 in cancer. A short peptide targeting the association of the two p53 inhibitors, MDM2 and MDM4, induces an otherwise therapeutically active p53 with unique features that promote cell death and potentially reduce toxicity towards proliferating nontumor cells.
Sonia Valentini, Giada Mele, Marika Attili, Maria Rita Assenza, Fulvio Saccoccia, Francesca Sardina, Cinzia Rinaldo, Roberto Massari, Nicola Tirelli, Alfredo Pontecorvi, Fabiola Moretti   +10 more
wiley   +1 more source

The effect of surface probe density on DNA hybridization [PDF]

Nucleic Acids Research, 2001
The hybridization of complementary strands of DNA is the underlying principle of all microarray-based techniques for the analysis of DNA variation. In this paper, we study how probe immobilization at surfaces, specifically probe density, influences the kinetics of target capture using surface plasmon resonance (SPR) spectroscopy, an in situ label-free ...
Richard J. Heaton, R. Georgiadis, Alexander W. Peterson   +2 more
openaire   +3 more sources