Results 71 to 80 of about 406,354 (305)

Stacking-induced fluorescence increase reveals allosteric interactions through DNA [PDF]

, 2018
From gene expression to nanotechnology, understanding and controlling DNA requires a detailed knowledge of its higher order structure and dynamics. Here we take advantage of the environment-sensitive photoisomerization of cyanine dyes to probe local and ...
Lopez, Sergio G., Magennis, Steven W., Morten, Michael J., Rafferty, Aidan, Steinmark, I. Emilie   +4 more
core   +1 more source

UPS 2.0: unique probe selector for probe design and oligonucleotide microarrays at the pangenomic/ genomic level

BMC Genomics, 2010
Background Nucleic acid hybridization is an extensively adopted principle in biomedical research, in which the performance of any hybridization-based method depends on the specificity of probes to their targets.
Hsiung Chao A, Kuo Bao-Han, Su Sheng-Yao, Lo Chen-Zen, Chen Shu-Hwa, Lin Chung-Yen   +5 more
doaj   +1 more source

Probe signal correction for differential methylation hybridization experiments

BMC Bioinformatics, 2008
Background Non-biological signal (or noise) has been the bane of microarray analysis. Hybridization effects related to probe-sequence composition and DNA dye-probe interactions have been observed in differential methylation hybridization (DMH) microarray
Yan Pearlly, Potter Dustin P, Huang Tim HM, Lin Shili   +3 more
doaj   +1 more source

Correlation of the differential expression of PIK3R1 and its spliced variant, p55α, in pan‐cancer

Molecular Oncology, EarlyView.
PIK3R1 undergoes alternative splicing to generate the isoforms, p85α and p55α. By combining large patient datasets with laboratory experiments, we show that PIK3R1 spliced variants shape cancer behavior. While tumors lose the protective p85α isoform, p55α is overexpressed, changes linked to poorer survival and more pronounced in African American ...
Ishita Gupta, Yang Song, Madeleine Ndahayo, Anirudh Saxena, Theresa Guo, Dylan Z. Kelley, Jessica Gore, Andrew Hennigan, Alexa Anderson, John C. Papadimitriou, Daria A. Gaykalova   +10 more
wiley   +1 more source

Application of COMPOCHIP Microarray to Investigate the Bacterial Communities of Different Composts [PDF]

, 2009
A microarray spotted with 369 different 16S rRNA gene probes specific to microorganisms involved in the degradation process of organic waste during composting was developed. The microarray was tested with pure cultures, and of the 30,258 individual probe-
A Alfreider, A Loy, A Loy, BEE Buchholz-Cleven, Brigitte A. Knapp, CJF Braak ter, D Guschin, D-Y Lee, DA Stahl, DM Barrett, EW Alm, F Schwieger, F Wintzingerode von, G Muyzer, G Taroncher-Oldenburg, G Varani, H Daims, H Sanguin, H Urakawa, HAJ Hoitink, HAJ Hoitink, HAJ Hoitink, Heribert Insam, HM Dionisi, IH Franke-Whittle, IH Franke-Whittle, Ingrid H. Franke-Whittle, J Peplies, J Ryckeboer, Jacques Fuchs, JG Fuchs, K Nakasaki, K-H Kim, KJ Breslauer, L Bodrossy, L Bodrossy, L Ludwig, M Blanc, M Danon, M Hogardt, MA Elorrieta, MT Suzuki, N Stralis-Pavese, P Legendre, P Schloss, PM Dees, R Amann, Ruediger Kaufmann, S Haruta, SF Altschul, SM Tiquia, SM Tiquia, T Beffa, W-T Liu, Y Koizumi   +54 more
core   +1 more source

32P Radioactive Probe Synthesis and Preparation

Bio-Protocol, 2011
To probe for a specific mRNA species by Northern blot, RNA from the agarose/formaldehyde gel needs to be transferred to a nylon membrane. RNA is detected by hybridization using a labeled probe.
Bio-protocol Editorial Office
doaj   +1 more source

Hybrid PET-optical imaging using targeted probes [PDF]

Proceedings of the National Academy of Sciences, 2010
Fusion imaging of radionuclide-based molecular (PET) and structural data [x-ray computed tomography (CT)] has been firmly established. Here we show that optical measurements [fluorescence-mediated tomography (FMT)] show exquisite congruence to radionuclide measurements and that information can be seamlessly integrated and visualized. Using
Nahrendorf, Matthias, Keliher, Edmund, Marinelli, Brett, Waterman, Peter, Feruglio, Paolo Fumene, Fexon, Lioubov, Pivovarov, Misha, Swirski, Filip K., Pittet, Mikael J., Vinegoni, Claudio, Weissleder, Ralph   +10 more
openaire   +3 more sources

Actin dynamics controlled by IqgC, a RasGAP at the crossroads between the IQGAP and fungal GAP1 families

FEBS Open Bio, EarlyView.
IqgC is a RasGAP from Dictyostelium discoideum. IqgC binds RasG via its RasGAP domain and deactivates it on macroendocytic cups, thereby suppressing the uptake of fluid and particles. IqgC has a positive effect on cell‐substratum adhesion, and its RGCt domain is required for recruitment to ventral foci.
Vedrana Filić, Darija Putar, Lucija Mijanović, Igor Weber   +3 more
wiley   +1 more source

Interplay between RNA‐protein interactions and RNA structures in gene regulation

FEBS Open Bio, EarlyView.
Methodological advances in mapping transcriptome‐wide RNA‐protein interactions and RNA structures have started to uncover the potential of RNP conformations in gene regulation. Competing RNA–RNA, RNA‐protein and protein–protein interactions shape the compaction and function of RNPs throughout their lifetime and may provide novel therapeutic targets in ...
Jenni Rapakko, Mauro Scaravilli, Minna‐Liisa Änkö   +2 more
wiley   +1 more source

Modeling and Estimation for Real-Time Microarrays [PDF]

, 2008
Microarrays are used for collecting information about a large number of different genomic particles simultaneously. Conventional fluorescent-based microarrays acquire data after the hybridization phase.
Hassibi, Arjang, Hassibi, Babak, Vikalo, Haris   +2 more
core   +2 more sources