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Analytical Chemistry, 2013
For establishing cells that secrete antibodies most efficiently (e.g., hybridomas, CHO (Chinese hamster ovary) cells), the screening and subsequent breeding of promising cells have been performed at the single-colony level, which requires several weeks ...
Akiko Kida +5 more
semanticscholar +1 more source
For establishing cells that secrete antibodies most efficiently (e.g., hybridomas, CHO (Chinese hamster ovary) cells), the screening and subsequent breeding of promising cells have been performed at the single-colony level, which requires several weeks ...
Akiko Kida +5 more
semanticscholar +1 more source
Antibody production by hybridomas
Journal of Immunological Methods, 1980J. Goding
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Role for c-myc in activation-induced apoptotic cell death in T cell hybridomas.
Science, 1992Immature T cells and some T cell hybridomas undergo apoptotic cell death when activated through the T cell receptor complex, a phenomenon that is probably related to antigen induced negative selection of developing T cells.
Yufang Shi +5 more
semanticscholar +1 more source
Chapter 7 Cell Hybridization, Hybridomas, and Human Hybridomas
1998Cell hybridization is one of the most basic cytotechnologies. The hemagglutinating virus of Japan was first used to cause cell fusion; however, polyethylene glycol is widely used now because of simplicity of procedure. This chapter first explains the principles of cell hybridization methods and then describes the practical protocols for preparing mouse
Kiichiro Teruya +2 more
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International Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biology, 1986
The attraction of monoclonal antibodies lies in the potential availability of an unlimited supply of material, homogeneity, reproducibility, and the feasibility of manipulation for specific purposes. Their use, however, is limited because of structural and physicochemical idiosyncrasies, occasionally restrictive factors influencing affinity and avidity,
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The attraction of monoclonal antibodies lies in the potential availability of an unlimited supply of material, homogeneity, reproducibility, and the feasibility of manipulation for specific purposes. Their use, however, is limited because of structural and physicochemical idiosyncrasies, occasionally restrictive factors influencing affinity and avidity,
openaire +2 more sources
Production of Hybridomas [PDF]
, 1992 To be suitable for fusion, myeloma and other tumor cells must fulfil the following four conditions: 1. They may no longer synthesize intact antibody or immunoglobulin light or heavy chains themselves. 2. They must possess an enzyme defect, to allow their selective elimination after the fusion. 3.
D. Baron +7 more
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Generation and maintenance of hybridomas
Immunology Today, 1987The realization of the therapeutic potential of monoclonal antibodies will depend on the efficient generation and maintenance of hybridomas. At a recent workshop on the Stability and Viability of Antibody-Producing Cell Lines it was clear that there are still large gaps in the knowledge needed to achieve that aim.
M.D. Scharff, J.F. Albright
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Polyspecificity of monoclonal lupus autoantibodies produced by human-human hybridomas.
New England Journal of Medicine, 1983We studied the serologic properties of monoclonal autoantibodies that were produced by hybridomas derived from lymphocytes of patients with systemic lupus erythematosus.
Y. Shoenfeld +6 more
semanticscholar +1 more source
European Journal of Immunology, 1984
AbstractA hybridoma is described that exhibits all the characteristic features of Mott cells. It has spherules (Russell bodies) in the cytoplasm made up of dilated rough endoplasmatic reticulum and containing condensed immunoglobulin (λ1 light chains). Some of the cells appear to be very fragile, and free spherules are often found on cell smears. Cells
Peter D. Burrows +3 more
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AbstractA hybridoma is described that exhibits all the characteristic features of Mott cells. It has spherules (Russell bodies) in the cytoplasm made up of dilated rough endoplasmatic reticulum and containing condensed immunoglobulin (λ1 light chains). Some of the cells appear to be very fragile, and free spherules are often found on cell smears. Cells
Peter D. Burrows +3 more
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2015
The generation of hybridoma cell lines by the fusion of splenocytes from immunized mice with immortal myeloma cells is a well-established method for the production of monoclonal antibodies. Although other methods have emerged as an effective alternative for the generation of monoclonal antibodies, the use of hybridoma technology remains a viable ...
Robert M, Hnasko, Larry H, Stanker
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The generation of hybridoma cell lines by the fusion of splenocytes from immunized mice with immortal myeloma cells is a well-established method for the production of monoclonal antibodies. Although other methods have emerged as an effective alternative for the generation of monoclonal antibodies, the use of hybridoma technology remains a viable ...
Robert M, Hnasko, Larry H, Stanker
openaire +2 more sources