Results 261 to 270 of about 59,780 (293)
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Coning Hybridoma cDNA by RACE

2001
V region primer PCR is usually successful in the amplification of hybridoma V genes, especially if using diverse primer sets (Krebber et al. 1997). However, there are a number of potential pitfalls in using V region PCR. Mutations within the 5′ or 3′ ends of the V genes may inhibit primer annealing and so prevent amplification.
Andrew Bradbury, Andrew Bradbury
openaire   +3 more sources

Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system.

JIM - Journal of Immunological Methods, 1997
A. Krebber   +6 more
semanticscholar   +1 more source

Rat × Rat Hybridomas

2003
There is an increasing interest in the preparation of rat × rat hybridomas, because they have been found to be more stable in culture than mouse hybridomas and they secrete consistently high levels (10 µg/mL and above) of monoclonal antibody. In addition, certain subclasses of rat IgG have been found to interact efficiently with human Fc receptors and ...
openaire   +3 more sources

Activation-induced cytidine deaminase turns on somatic hypermutation in hybridomas

Nature, 2002
Alberto Martin   +5 more
semanticscholar   +1 more source

Polyspecificity of monoclonal lupus autoantibodies produced by human-human hybridomas.

New England Journal of Medicine, 1983
Y. Shoenfeld   +6 more
semanticscholar   +1 more source

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