Results 271 to 280 of about 59,735 (325)
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2005
Hybridomas can be grown in a number of ways to produce stocks of monoclonal antibodies. Smaller volumes may be produced in static flask culture, but if larger amounts are required, a bioreactor may be used.
Gary, Entrican, Gareth, Young
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Hybridomas can be grown in a number of ways to produce stocks of monoclonal antibodies. Smaller volumes may be produced in static flask culture, but if larger amounts are required, a bioreactor may be used.
Gary, Entrican, Gareth, Young
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Monoclonal Antibodies of Hybridomas
Clinical Infectious Diseases, 1983Among the most prominent biological advances of this century, the hybridoma technology stands out as providing the best tool for the analysis of complex antigens of pathogens and tumor cells. This technology will play a major role in the immunodiagnosis and treatment of infectious and malignant diseases.
Joseph G. Sinkovics, Gordon R. Dreesman
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2005
Hybridomas are derived from the fusion of spleen and myeloma cells and produce monoclonal antibodies. Each hybridoma cell line produces an antibody with a unique specificity allowing the production of highly defined reagents that can be used in many branches of immunochemistry.
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Hybridomas are derived from the fusion of spleen and myeloma cells and produce monoclonal antibodies. Each hybridoma cell line produces an antibody with a unique specificity allowing the production of highly defined reagents that can be used in many branches of immunochemistry.
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Cryopreservation of Hybridomas
2003Hybridomas are exposed to many threats, such as contamination with bacteria and fungi, loss of chromosomes coding for antibody production, overgrowth by nonsecreting mutants, and cell death resulting from overgrowth. Therefore, newly established hybridomas should be frozen and stored in liquid nitrogen at -196°C as soon as possible. Alternatively, they
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Hybridomas As a Source of Antibodies
Hospital Practice, 1981Hybridomas, the progeny of fusion between antibody-secreting spleen cells and myeloma cells, produce large quantities of a single species of antibody. They are an ideal source of serologic reagents since they can be grown in tissue culture, frozen, stored, and recovered when needed.
Pallaiah Thammana +2 more
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Cryopreservation of hybridomas
Journal of Tissue Culture Methods, 1985A method for the long-term storage of hybridoma cells in liquid nitrogen is described. Hybridoma cells are harvested in logarithmic phase of growth and suspensed in freeze medium containing dimethylsulfoxide. Using a programmable freezer, the cells are frozen very slowly (1°C/min) and then transferred rapidly to liquid nitrogen refrigerators ...
M. Irene Cour, Yvonne Reid
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Journal of Immunology, 1989
By establishing hybridomas from two distinct surface IgM+ splenic B cell populations, Ly-1 B cells and "conventional" (Ly-1-) B cells, we found that the Ly-1 B population includes a 30 to 70 times higher frequency (1 to 2%) of cells with specificity for ...
Richard R. Hardy +4 more
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By establishing hybridomas from two distinct surface IgM+ splenic B cell populations, Ly-1 B cells and "conventional" (Ly-1-) B cells, we found that the Ly-1 B population includes a 30 to 70 times higher frequency (1 to 2%) of cells with specificity for ...
Richard R. Hardy +4 more
semanticscholar +1 more source
2003
Hybrid clones will appear within 2-3 wk after fusion (see Chapter 46). It is of utmost importance that a newly established hybridoma is cloned thoroughly to ensure that the cells growing in the tissue culture are of monoclonal origin and not a mixture of two or more hybridomas.
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Hybrid clones will appear within 2-3 wk after fusion (see Chapter 46). It is of utmost importance that a newly established hybridoma is cloned thoroughly to ensure that the cells growing in the tissue culture are of monoclonal origin and not a mixture of two or more hybridomas.
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In Vitro, 1981
The National Institute of Allergy and Infectious Diseases is developing, with the American Type Culture Collection, a central facility for the acquisition, maintenance, and distribution of hybridoma cell lines. This effort finds its principal justification in the great activity in this field and in the large variety of hybridoma lines that are produced
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The National Institute of Allergy and Infectious Diseases is developing, with the American Type Culture Collection, a central facility for the acquisition, maintenance, and distribution of hybridoma cell lines. This effort finds its principal justification in the great activity in this field and in the large variety of hybridoma lines that are produced
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Stable antibody-producing murine hybridomas.
Science, 1983A method is described for obtaining antibody-producing hybridomas that are preferentially retained in cultures of fused mouse spleen and myeloma cells.
R. Taggart, I. Samloff
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