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Production of Hybridomas [PDF]
, 1992 To be suitable for fusion, myeloma and other tumor cells must fulfil the following four conditions: 1. They may no longer synthesize intact antibody or immunoglobulin light or heavy chains themselves. 2. They must possess an enzyme defect, to allow their selective elimination after the fusion. 3.
D. Baron +7 more
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International Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biology, 1986
The attraction of monoclonal antibodies lies in the potential availability of an unlimited supply of material, homogeneity, reproducibility, and the feasibility of manipulation for specific purposes. Their use, however, is limited because of structural and physicochemical idiosyncrasies, occasionally restrictive factors influencing affinity and avidity,
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The attraction of monoclonal antibodies lies in the potential availability of an unlimited supply of material, homogeneity, reproducibility, and the feasibility of manipulation for specific purposes. Their use, however, is limited because of structural and physicochemical idiosyncrasies, occasionally restrictive factors influencing affinity and avidity,
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Polyspecificity of monoclonal lupus autoantibodies produced by human-human hybridomas.
New England Journal of Medicine, 1983We studied the serologic properties of monoclonal autoantibodies that were produced by hybridomas derived from lymphocytes of patients with systemic lupus erythematosus.
Y. Shoenfeld +6 more
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European Journal of Immunology, 1984
AbstractA hybridoma is described that exhibits all the characteristic features of Mott cells. It has spherules (Russell bodies) in the cytoplasm made up of dilated rough endoplasmatic reticulum and containing condensed immunoglobulin (λ1 light chains). Some of the cells appear to be very fragile, and free spherules are often found on cell smears. Cells
Peter D. Burrows +3 more
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AbstractA hybridoma is described that exhibits all the characteristic features of Mott cells. It has spherules (Russell bodies) in the cytoplasm made up of dilated rough endoplasmatic reticulum and containing condensed immunoglobulin (λ1 light chains). Some of the cells appear to be very fragile, and free spherules are often found on cell smears. Cells
Peter D. Burrows +3 more
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Generation and maintenance of hybridomas
Immunology Today, 1987The realization of the therapeutic potential of monoclonal antibodies will depend on the efficient generation and maintenance of hybridomas. At a recent workshop on the Stability and Viability of Antibody-Producing Cell Lines it was clear that there are still large gaps in the knowledge needed to achieve that aim.
M.D. Scharff, J.F. Albright
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2015
The generation of hybridoma cell lines by the fusion of splenocytes from immunized mice with immortal myeloma cells is a well-established method for the production of monoclonal antibodies. Although other methods have emerged as an effective alternative for the generation of monoclonal antibodies, the use of hybridoma technology remains a viable ...
Robert M, Hnasko, Larry H, Stanker
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The generation of hybridoma cell lines by the fusion of splenocytes from immunized mice with immortal myeloma cells is a well-established method for the production of monoclonal antibodies. Although other methods have emerged as an effective alternative for the generation of monoclonal antibodies, the use of hybridoma technology remains a viable ...
Robert M, Hnasko, Larry H, Stanker
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2005
Hybridomas can be grown in a number of ways to produce stocks of monoclonal antibodies. Smaller volumes may be produced in static flask culture, but if larger amounts are required, a bioreactor may be used.
Gary, Entrican, Gareth, Young
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Hybridomas can be grown in a number of ways to produce stocks of monoclonal antibodies. Smaller volumes may be produced in static flask culture, but if larger amounts are required, a bioreactor may be used.
Gary, Entrican, Gareth, Young
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Monoclonal Antibodies of Hybridomas
Clinical Infectious Diseases, 1983Among the most prominent biological advances of this century, the hybridoma technology stands out as providing the best tool for the analysis of complex antigens of pathogens and tumor cells. This technology will play a major role in the immunodiagnosis and treatment of infectious and malignant diseases.
Joseph G. Sinkovics, Gordon R. Dreesman
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Journal of Immunology, 1989
By establishing hybridomas from two distinct surface IgM+ splenic B cell populations, Ly-1 B cells and "conventional" (Ly-1-) B cells, we found that the Ly-1 B population includes a 30 to 70 times higher frequency (1 to 2%) of cells with specificity for ...
Richard R. Hardy +4 more
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By establishing hybridomas from two distinct surface IgM+ splenic B cell populations, Ly-1 B cells and "conventional" (Ly-1-) B cells, we found that the Ly-1 B population includes a 30 to 70 times higher frequency (1 to 2%) of cells with specificity for ...
Richard R. Hardy +4 more
semanticscholar +1 more source
2005
Hybridomas are derived from the fusion of spleen and myeloma cells and produce monoclonal antibodies. Each hybridoma cell line produces an antibody with a unique specificity allowing the production of highly defined reagents that can be used in many branches of immunochemistry.
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Hybridomas are derived from the fusion of spleen and myeloma cells and produce monoclonal antibodies. Each hybridoma cell line produces an antibody with a unique specificity allowing the production of highly defined reagents that can be used in many branches of immunochemistry.
openaire +2 more sources